Ppl (NM_001106976) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213102 representing NM_001106976 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCGCTCTTCAGGAAGAAAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCGGACCAGGTGGAAAGGAACATTGTGGA CACCGAGGCCAAGATGCAGAGTGACTTGGCCCGGATGCAAGACGGGCAGCTCCCTGAGCACCGGGACGCG ACCCTGCAGAATGTGTCTGACTCAGAAAAGCTGCTGTACGTACTGGAGGCAGACTCAGCCATCGCCAAGC ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGTGTGACCAACCT GCGGGGCAAACACAAGCAGATGTACAGCCTGGCAGTGAGAGAGGCTGACCCGAAGGTCAACTGGGATGCA CTGGTGGATGAGAAACTGGACAAGCTGAGCAGCCAGAGTTTTGGGACTGACCTTCCTCTGGTGGACAGCC AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA GCAGAACAGCGAACTCCAAGCCAAGTATCAGAAGTTGCTGGCAGCATCACAGGCTCGGCAGCAGCACCTG AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC GCATACAGTATGACTGGAGTGACCGCAACCTCGACTACCCCAGCCGCCGGCGCCAGTACGAGAATTTCAT CAACCGGAACCTGGAGGCCAAAGAAGAGAGAATCAACAAACTGCACACTGAAGGCGACCAGCTGCTGACA GCCGAGCACCCGGGGAGGAACTCCATTGAGGCACACATGGAGGCGGTGCACGCGGAGTGGAAGGAGTACC TGAACCTGCTCATCTGTGAGGAGAGCCACCTGAAATACATGGAAGACTATCACCAATTTCACAAAGATAT GAAGGATGCTCAGGAACTGCTGCGCAAGGTGGACTCAGACTTGAACCAGAAATACAGCCCTGACTTCAAA GACCGATATCAGATAGAGCTGTTGCTTCGGGAGCTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG ATGTGGTGCGGGGGCTGCAGAAGCGAGGGCAGCAAGTGCTGCCACTCAAGTACCGGCGTGAGACGCCACT CAAGCCCATCCCTGTGGAGGCCCTCTGTGACTTTGAGGGTGACCAGGGCCTAATCTCCCGGGGCTACAGC TACACGCTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGATGGCCC CAGCTGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTCTCGGACAGCCTGGGCAGCCA GTACCGGAGTGTGCGGCAGAAGGCGGCCGGGAGCAAACACGCGCTCCAGCAGCGGCATGAGGTGCTAAGG ACAGAGAACCCTGGAGATGCCTCCGATCTTCAGGGGCGACAGCTGCTGGCTGGCTTGGACCAAGTGGCCA GTGACCTGGACCGGCAGGAGAAGGCTATCACGGGGATCCTACGGCCACCCCTTGAGCAGGGACGGGCAAT AGAAGATAGTGCTGAGCGGGCCAAGCACCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG AAGATACAGCGCACAGCTGAGTGTGAGGCCTTCGTGCAGGCCCTCCCGGGCAGTGGCACCACACCCTTGC TGAAGACCCGGGTGGAAGATACCAACCAGAAATATAAGCGACTGGTACAGCTGCTAGAAGCAGCCCAGGA GAAGGTGGATGTTGCCAACCGCCTGGAGAACAGTCTGCAACGGGGCAGGGAGCTGCTGGCCTCCCACGAG AACAGGCTGATGCAGGATGACACAATACCTGAAAGTGACTACATGTTGGACAGCAAGAGGCAGGAGCTAG AGGCCATGGCTTCTGAGCTGCAGGCCCAGAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTGCAGGTGGC CAAGCAGTGCTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGCCAGGAGGCT GAGGTACACAAGCTCAACCAGCGTTTCAACAACCTCAATCAGCAGGTGGAACGCAGGGCCCAGAGCCTGC AGAGTGCCAGGGATGCCTACAGTGAATACTGCCGAGGTTATGACCATGTGCTTCAGTTCTTGGTCAAGAC TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCAGCCAGATGGAGACCAAACTGAAGAACCAGAAGAAC TTGCTTGATGAGATAGCAAGCATGGAACAAGGGGCGCAGAAGGTCTACGCAGACTCCCAACAGTACCAGC AAGCAGTGAAGGACTATGAGCTTGAAGCAGAGAAGCTAAGGTCCCTGCTGGACCTGGAGAACGGACGGAA CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT GCTGCCAAGTTCACAGAAGTTAATGCCATCAACAGACAGAGGCTACAAAATTTGGAGTTTGCACTGAATC TCTTGAGACAGCAACCAGAGGCAGGAGTGACCCATGAGACTCTGCAAGGGAGAGAGCAAGGCTCTGGCAT GGAGGAAACATGGAAGATTCAGAAGGAACTGGAGGAGGAGATGGAGCGAAGGCAACAGTTGGAAAATGAG GTCAAGAGTGCCCAGGAAGAAATCCAGACCCTGAAGGATCAGGGTCCTCAGGAATCATTGGTGAGGAAGG AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACCCTGGCTGA GGAACAGCACAAGAACCAGCTGTTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCCCTAGAA CAGGAGACCAGGGATGGGGGACAAGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC AGGAGGATGAGGTTTTACAGCTTCGGGAGGAGCTGGAGGGACTACGGCGCCAGAAAGGTGCCCGAGAGGC TGAGGTACTACTCTTACAGCAGCGCGTAGCAGCCCTAGCTGCTGAGAAGAGCAGGGTACAGGAAAAGGTC ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCTCAGCTGGAGGCAGAATACCGGAGGCTACAGGAGG AACACCAACGGGAAGGTACACTCAGGGAGAAGCAGGAAGAGGAGCTGAGTTTCCTACAGGCCAAGCTCAG GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAAGTAGAGAAA GATACAGCTGCAGAGAGGGAAGTCAATGACCTCAGCCGCCAGTATGAGGATGAGGCTTCCAAGGCTCGCA CTAGCCAGCGGGAGAAGACAGAGCTTCTCAGAAAGATATGGGCCCTGGAAGAAGAAAATGCCAAGGTGGT GGTACAGGAGAAGGTCCGAGAGATTGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGAGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC TGGAGGCCCTACGGAACCGCGGGCCACAAGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATACAC AACTGACCCAGAGACGGAGCAGGAACTCCAGCGACTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA GAAAGGTGTGACTTAGAGATTTATCAGCTGAAGCAGGAGATCCAAATCCTGAAAGACACCAAGCCACAGG TGCAGACTAGAGAGGTGGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA GTCTCTGCGTATACAGCTGTCAGAAGAAAAGAAGAAACTGGTGGACCTGGAAGGGGAGCAGGCATCCCAG GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCCCAGGGTAAGGAAAGGATTGTACGCCAGGAGGTGG TACAGTATGAAGATGAGCCAGATTTGCGGGCTGAGGTGACTGCCTTCACAGACAGCATTGATGTTGAGCT GAGGCAGATTGATAAACTGTGTGTGGAGCTACGGCGGCTACAGCATCGCAGAGCAGAGCTGGAGAGGCAG CTGGAGGAGCTGGAGCGTGAGAGGCAGGCACGTAGGGCAGCTGAGCTGGAGGTGCAGAGGCTGCAACAGC GGCTGGCTGCACTGGAGCAAGAGGAGGCCAAGACTGGTGAGAAAGTGACCCACACACAAAAGGTGGTGCT GCAGCAGGACCCACAGCAAACCAGGGAACATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGACACCGG AGGCAGCTACTGGAGGGTGAGCTTGAGCCCCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGACAGAGATCA AAGAAAAGGTGGTCTTCTCCGAGAGTGTCCAGGTAGAAAAGGGTGACACTGAGCAAGAGATCCAGCGGCT CAAGAAGAGCCTGGAAGAGGAGAGCCGGAGCAAGAGGGAGCTAGATTCAGAGGTGACCCGGCTGGAAGCC AAGTTATCTGAGCTAGAATTCTACAATTCCAAGTCATCCAAGGAACTGGATTTCCTGAGAGAAGAAAACC ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC CGCAACAGAAACTCGAGATCTGAAGAACATAACCATGGTAGACTCTGGAACCCACCTAAACTCTAGGCTG TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC TGCAGAGGCGTCTGGGCTCTGTAGCCGTCAAGAGGGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT GGTCATTGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCCCACCGGGCTGGGCTCATCGACTGG AAAATGTTCGTGAAGCTCAGAAGCCAGGAGTGTGACTGGGAAGAGATATCAGTGAAAGGTCCTAATGGGG AGTCATCAGTAATCCATGACAGGAAGTCTGGCAAGAAGTTTTCCATTGAGGATGCTCTGCAGAGTGGCAG GCTAACCCCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG TCTGGGCAGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106976
Insert Size	5265 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106976.1, NP_001100446.1
RefSeq Size	6281 bp
RefSeq ORF	5265 bp
Locus ID	302934

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.