Sphkap (NM_001127492) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1311951; Skip
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213119 representing NM_001127492 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAACTCCCGGCTTTCTGTGCAAAGCCATGTGGGTAGTTGAACCAAACTCTGCTACCTGCCTTTCTG CTGCCCCGCCGGTCAAGAGACACTGCCAAGAATCCAGCAACGTGGAATCACCACTGATGCCCGAGGACTC GGAGCCACAGCAGATCACCAGCAGTGCAGCAGGCAGCCTGGCTGGCTCCATCACGGCCTGTAAGAAGGTT CTTCGAAGCAATAGCTTGCTGGAGTCCACAGACTACTGGTTGCAGAACCAGAGGACGCCTTGCCAGATTG GGTTTGTGGAAGACGAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAACCTGGATGTCAACAAGGATGG CTGCAGCACAGAGAACCTGCAGCAGAAACTGGTCAACGTTTCACCAGACCTTCCAAAACTCATCAACTCC ATGAATGTCCAGAAGCCAAAAGAAAATGAAATTGTCCTCCTAAGTGGGCTAGCCTCTGGAAACCGCCAGG CAGATTTTGATGTTTCACAGTGCCCTTGGCTACCAGATATCTGCTTGGTCCAGTGCGCAAGAGGGAACAG ACCAAACAGTACCAACTGCATCATCTTTGAGATCAACAAGTTCTTAATTGGTCTGGAAGTGGTGCAGGAG CGACAGCTCCACCTGGAGACCAGCGTGTTGAAGCTGGAGGATGATACAAACTGTTCCCTGTCTTCGATTG AAGAAGATTTCCTTACAGCCTCAGAGCATTTGGAGGAAGAAATCGAGGTGGAAGACTGCAAAAGTGGTTT GGAAACTATAAATGTGTCAGCTAACGTTCTGGAAAGCAAAAGGCCAAAGGAAGCTACCCAGGAAGGATGG GATTACCACAAGGAAATATTGCATTGTGCTTTAGGTGAAAAACACATCAGAAAACACCGTATGCCCTCAA TGAAAACAGAAAGGTCAAAGGAAAACACAGCAGAGAACACAGCCCTGCAAAGTCTCAATCCTTCAGCCAG ACCATCACGCTTGAAAAGTGAAGTGGCGGGGAGCAAGCAGCCAGCCACGAACTACTCTTACCCCGAAAAT ATCAGAGGAGAAATAGAAACATCTCAGATGCTGTTCATCCCCAGGGATGCCTATCTCTCCATGGTTTCTT CATGTGGTGTACTCACTGAGCAGGGAAGCAACCACAGAGACCATGACGCCACTCCAAACTCCCTTCCTCC CGTTCAAAATGGAGAAGCCACCGCGGGCGAGTACGCTACCAACTTAGCAGAATCTGTAATGCAAGATGCC TTCATCCGATTGTCTCAGTCGCAACCTACACTCCCCCAGGAATCTGCAGTCAGTTTCTCTGTGGGAAGCG CTCTGCTCCCCAGTGGTTGTTGCACCAAAGATATGGTGGTCCCTCGTTCATGGAATGAGCTTCCTAAAAT TGTCATCGTGCAGAGCCCAGAGGGCAGCGACACAGCCCCTGAGCCAAGTGTCTCCTCCTGGCCCGACCTG GAAGTGTCTGTCGAGACCCCGGGCATCTTTTCTGAAGAGAGCTCCAGCAGACCCACTCAGAGTGCCCTAG AAGTTGCTTTGGCTTGTGCAGCCACTGTGATTGGAACCACTTCCAGTCCACAGGCCACAGAAAGATTCAC CATGGAGCAAGAATCTCTGGTATCTACCTACACCCAGAGAGGCAATGGGATACAGCAAACTCCAGTGCCC AGAGTATTCATGGGACCTTCTACAACTGAATACTCCTTCCCATCAGCTCTGTGTGGCATGACCCAAGTGG CAAGCGCTGTTGCTGTCTGTGGCCTAGGGGAAAGAGAAGAGGTGACATATTCTGTAGCTCCAAGTGACCT CCTGCCCACACCTGGGGCTTCTGAAGAAAGATCCTCCATTGGAAGTTTAGTGACAGAGGAAAGCACAGAA CTGGGGAAGGAAGCCATTGCAGAGGCTCTGCTCAGGGAGGCTACTCTGGTTTTAGCGAGGCCGAATGCAT ACAGCAGCGTCGGAGAGCTCTTGGAATCTGTGAACCGGAGGATCATAGAAACCACTTCTAAAACCCAGAT GCTGTGCACAGAAAATGTTCGAAGGAATGAACTGGCACACACCTTGTCCAATGTCATCCTCAAGCATTCC ATTGATGAGCTGCACCAGAAAAATATCATGGCTCACCCCACCGAGGAAAGGCATCCCGGTGGGACTCTGA ACACCTTAATGGAAAGTGTGAACCAGTTGCTGCACAACGTGATGTGCTTCACCTTCAGAAAGATGAATCA CATCGTAACACTGGGTGAGCATCCCTCATTTGATAAGGCTGCTGGTCATGCATGGGTTAAAGCCTCGGCT TGCTCCAGCAGCCATCCCTCTAGCAATGCACACGGTACTGGCCTTGTCATCAGAGATCTTGTAGAAGATG CATCTCCTAAGCCAGACAAGGGAGGAGCAAGGCCAGAACTGGTCAACAACCCCAGGCTGCAATCTGAATT CTCATGCAGTCACAGAATGCTTGATTCAACTGCTAGGACATTCCCCAAGGAAATGTATCCAAAAGGGATT GTGGGAGAGGATACAAGAAACCCTCTTCATACACTAAGTTATGACAGCAGTGAACAAAGAACTTCTACAG ATATAGGAAAATTAACAACAGTAGGAGAGGTCCGTAGTACCTTCCAGGAATCTGAAGACAGCATTGTTCC AAAAGCCCAAGAGAAACACACTTGTGCCACAACTCTAAACAACGAAGCTCAAATTAACCTATCCTTGTTA GGGGATGACCTAGTGGTTCCTGATCAATCTACACTAGAGGCAAAGCAGTCCGAGGTCTATGGCATCACAA ACTTTGCGGAAGAATTGGCAGAGACTGTTGTCTCCATGGCAACTGAAATCGCAGCAATCTGCCTTGACAA CTCCCATGGAAAACAGCCGTGGTTTTGTGCTTGGAAGAGAGGGAACGAATTTCTGATGACGCCTAACGCA TCCTGCCGATCCTTGAAGAGGAAGAAGGAAAACTCGGGCACGGGAAGCACTGTGAGGAAACACAAGCCAC CTCGCCTCAGTGAGATCAAGAGAAAAGCAGATGAGCACCCAGAGCTGAAAGAGAAGCTGATGAACAGAGT CATGGATGAGTCCATGAACCTCGAAGACATTCCCGATTCCGTCAGTACTTTTGCAAATGAAGTGGCAGCC AAGATCATGAACCTAACAGAATTCTCCACGGTGGATGGAGTGTGGCAAACCCAGAGTTGTTCCCGGAATC GGCTTCTGGGTGGTGATAGGTGGAACCGGCTGAAGGCCTCGAGCTGTGAAAGTATTCCGGAGGAGGACTC TGAAGCCAGGGTCTTTGTAAACAGCCTGGGTTTAATGAGTACCTTAAGCCAACCCGTTAGCAGGGCCAGC TCAGTCTCCAAACAGTCCAGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAAACAGATGG AAAACGAAGGGAGGGGGTTCGAGTTGCTGCTGGATTACTACGCTGGCAAAAATGCCAGCAGTATCCTGAA CTCAGCAATCCAGCAGGCATGCCAGAAAAACGACCACCTCAACGTAAGACCAAGCTGCCCCTCTAAGCAG TCCAGCACAGAAAGCATAACAGAGGAGTTCTATAAGTACATGCTGAGAGACATTGCCAAAGAAAACAAAG ATGGCGCCCTGTCCAGACGAAGCAGCCATGATTGGAGCACGGGCTTGCTGTCTCCTTCCGCGCGATCCCC ACTGTGTTACAGGCAGTCATCTATGCCTGACAGCAGGTCCCCGTGCTCCAGGTTAACAGTAAATGCACCT ATCAAAGCCAACTCCTTAGATGGTTTTGCCCAAAACTGCCCTCAAGATTCTATAAATGTACAGCCAGTCA GTAGGGCTTCCTCGTCTGGCCTCTGCAAATCAGACTCCTGTTTATATCGCCGAAGTGGGACTGACCAGAT CACAAACATGCTAATTCATGAAACATGGGCCAGCTCCATTGAGGCTCTCATGAGAAAGAACAAAATCATT GCTGATGACGGCGAGGCAGCTAACGCTAGCCCTGGTCCTGTTTCTGGCGGCTCGCCTTCGCAAGTAGAGA AGTGTGCCAACAGACTAGTCACCGGCACAGGACACAAGGGACCAGCTCTACTTGTACAGGAGTCTGTTGA TTACCAGAGAAAGGATGCTGTCACTGAAGGCAATTGTTCCCCAGTGTCATCTCCAAGCAAAATGGCTCCT GTTAAAAAGCCCAGTGGTTTTGATCCTACAAGAGAAACCTCTGCATGCCACAATGCTGTTGCTCTCAAAA GCCCCAGGCGGTCTCTTTGCTCAAGGGAGGTGCCTTTGATTCAGATAGAGACCGATCAGAAAGAAGAATG CGTTGGAGAATCTGAAACCTTACTTCCCCAAAGTGGCTCCCTAGAGGAAGCAGAAGGGCCCCAGCCTGAG GAAACCATCCCAGATGTGGCCAGAAGCGAAGACACAGCCCTGAGCGCCTGTCAAAGCTCTCATGACAGCC TTGAGACCAGAGAAGAATTAGAAGTGGATGTCTTGAAAGAAGACATAACCCTAGATGAGTCCCGGAACCC TCCTAGCAGCAGCGAAGAGAGTACAGGCAGCTGGTCCCAGCTGGCCAATGAGGAGGACAACCCAGATGAC ACAAGTAGCTTTCTGCAGCTCAGTGAGCGGTCCATGAGCAATGGCAACACTAGTGGCACTAGCAGTCTTG GCATTATGGACCTGGACATTTATCAGGAAAGCATACCGTCTTCTCCCATGATTAATGAATTAGTAGAAGA AAAGGAGATTCTTAAAGAACAATCAGAAAGCGTAAAGGAACGTGCCTCTGGACTGCCAGGGAGAGCAGCC AGCCCCCAGAGGAGTCTACTGGTGATCAACTTTGACCTGGAGCCAGAGTGTCCTGATGCTGAGCTGCGAG CCACTCTGCAGTGGATAGCTGCCTCCGAGCTGGGCATCCCAACAATCTACTTTAAGAAATCTCAGGAAAG CAGAATTGAAAAGTTTCTAGATGTTGTGAAGCTTGTTCATCAGAAGTCCTGGAAGGTGGGAGATATATTT CATGCCGTTGTCCAGTACTGCAAAATGCATGCAGAGCAGAAAGAGGGAACTCCGAGTCTCTTTGACTGGC TCCTGGAACTAGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001127492
Insert Size	5127 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001127492.1, NP_001120964.1
RefSeq Size	5423 bp
RefSeq ORF	5127 bp
Locus ID	316561

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