Cacna1e (NM_019294) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CACHA1E; Cav2.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213444 representing NM_019294 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGACCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA GGAACCGACAAGGAACCCCCGTACCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG GGCGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTGTTC ATCTTCGGAGAAGATAACATTGTCAGGAAATATGCCAAGAAACTCATCGATTGGCCGCCATTTGAGTACA TGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGACAA GACCCCAATGTCCCGAAGACTGGAGAAGACAGAACCATATTTTATTGGAATCTTCTGCTTTGAAGCTGGG ATCAAAATCGTGGCTCTAGGATTCATCTTCCATAAGGGTTCGTACCTCCGTAACGGCTGGAATGTCATGG ACTTCATTGTGGTCCTCAGTGGCATCCTGGCCACTGCAGGAACCCATTTCAACACCCACGTGGACCTGCG GACCCTCCGGGCTGTGCGTGTCCTGAGGCCCTTAAAGCTCGTGTCAGGAATACCTAGCCTGCAGATCGTG CTGAAGTCCATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATTCTCA TGTTTGCCATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTCAGG TATTCTAGAGGGATTTGATCCTCCTCACCCGTGTGGTGTACAGGGCTGCCCGGCTGGTTATGAATGTAAG GACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCTGTGCTGACTGTCTTCC AATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTGGAA CTGGCTGTACTTCATCCCCCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTTGTCCTGGGCGTGCTT TCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCATTCATGAAGCTCCGGCGTCAGC AACAGATTGAGCGCGAGTTGAATGGCTACCGCGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGCTGA AGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGGACG GAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTGCCA GAGCCAGTATCAAGAGCACAAAAGTAGACGGAGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCGTAT CTCTATCCGACACATGGTCAAGTCGCAAGTGTTCTACTGGATCGTCCTGAGTGTTGTGGCTCTCAATACT GCCTGTGTGGCCATCGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAATTTT TGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTTTATTTTCA CTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTCGTTTGGGCAATCTTC CGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAACCA AGTATTGGGCATCCCTCCGGAATCTGGTTGTTTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTTACT CTTCCTCCTCTTCCTCTTCATTGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTTAAC TTTAATGATGGGACTCCTTCGGCTAATTTTGATACCTTCCCTGCAGCTATCATGACAGTGTTCCAGATCC TGACGGGGGAAGACTGGAATGAAGTGCTGTATAACGGCATCCGCTCCCAGGGTGGGGTGAGCTCAGGCAT GTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACACTGCTGAATGTATTCTTGGCT ATCGCTGTGGACAATCTCGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCTTCA ACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCATTGA GAGGGAGAGGAGACGCCGACACCACATGTCTGTGTGGGAACAGCGCACCAGCCAGCTGAGGAGGCACATG CAAATGTCTAGCCAAGAAGCCCTCAACAAAGAGGAGGCCCCACCAATGAACCCCCTCAATCCACTCAACC CACTGAGTCCTCTCAACCCACTCAATGCTCATCCTAGCCTCTATCGGAGGCCCAGGCCCATTGAGGGCCT ACCCCTGGGCTTGGGTCTTGAAAAATGTGAAGAGGAGCGTATCAGCCGTGGAGGTTCTCTCAAGGGGGAC ATAGGTGGCCTGACTAGCGTCCTGGACAACCAGAGGAGCCCTTTGTCCCTGGGCAAACGGGAGCCACCGT GGCTGCCCAGATCCTGTCATGGAAACTGTGACCCAACCCAGCAGGAGACTGGGGGAGGAGAGACTGTGGT GACTTTTGAGGACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAG GGCAAGGAGTCTGCCTCTGCCTCCAGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGT CCATCGATGGGGAGAAGGAACACGAGCCTCAGAGCAGCCACAGGAGCAAGGAGCCAACCATCCATGAAGA AGAGAGAACTCAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCAGGACTGGTAGGAGCT CTTGATGAGGCAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGA CAGAGCAGGAGGCTGAAGGCAGCAGCGAGCAAGCCCTGCTTGCGGATGTACAGCTGGATGTGGGCCGGGG CATTAGCCAGAGTGAACCTGACCTCTCCTGCATGACAACCAACATGGACAAGGCTACCACTGAGAGCACC AGTGTCACAGTCGCCATCCCTGACGTGGACCCCTTGGTGGACTCAACAGTGGTGAACATTAGCAACAAGA CGGATGGAGAAGCCAGTCCCTTGAAGGAAGCAGAGACCAAAGAGGAAGAGGAAGAGGTGGAAAAGAAGAA GCAGAAGAAGGAGAAGCGGGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACC ACTAACCCGATCCGCAAGGCTTGCCACTACATTGTGAATCTACGCTACTTTGAGATGTGCATTCTCTTGG TGATTGCGGCCAGCAGCATTGCCCTGGCAGCTGAGGACCCTGTACTAACTAACTCAGAGCGTAACAAAGT CCTGAGATATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTGATAAAGATGATAGACCAA GGCTTGATCCTGCAGGATGGATCGTACTTCCGAGACCTCTGGAATATCCTGGACTTTGTGGTAGTGGTTG GTGCATTGGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGCGGGATATCAAGACGATCAA GTCTCTGAGGGTACTTCGAGTTCTGCGACCACTGAAAACCATCAAGCGCTTGCCCAAACTGAAGGCTGTG TTTGACTGCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTTATGTTCA TCTTTGCCGTCATTGCTGTTCAGTTATTCAAGGGAAAGTTCTTTTATTGCACCGACAGTTCCAAGGACAC AGAGAAGGAATGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAGTGG AAGCGTCATGAATTCCACTATGACAACATCATCTGGGCCCTGCTGACCCTCTTCACCGTCTCCACGGGGG AAGGATGGCCTCAGGTTCTGCAGCACTCGGTAGATGTGACAGAGGAGGACAGAGGCCCAAGCCGCAGCAA CCGCATGGAGATGTCCATCTTCTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTC GTGGCCCTCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTAGAAAAGA ATGAGCGGGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCA CACCTTCCAGTACCGCGTGTGGCACTTCGTAGTCTCGCCATCCTTTGAGTATACCATCATGGCCATGATT GCCTTGAACACCGTTGTGCTGATGATGAAGTACTATTCTGCTCCTTGGACCTATGAGCTCGCCCTAAAGT ACCTGAACATCGCCTTCACTATGGTGTTTTCCCTAGAATGTGTCCTAAAGGTCATCGCTTTCGGCTTCTT GAACTATTTCCGAGATACCTGGAACATCTTTGACTTCATTACCGTGATCGGCAGTATCACAGAAATTATC CTGACAGACAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGGAGCTCTTCCGAGCTGCAC GGCTCATAAAGCTTCTGCGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAA GGCTCTCCCCTATGTCTGCCTTCTGATTGCCATGCTTTTCTTCATCTACGCTATCATTGGGATGCAGGTG TTTGGAAACATAAAGTTAGATGAGGAGAGTCATATCAACCGGCACAACAACTTTCGGAGTTTCTTCGGGT CTCTCATGCTGCTTTTCAGGAGCGCCACAGGGGAGGCTTGGCAGGAGATTATGTTGTCATGTCTGGGCGA GAAGGGCTGTGAGCCTGACACCACTGCACCCTCAGGGCAGAACGAGAGTGAGCGCTGTGGCACTGACCTG GCCTACGTTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTTGTGGCTGTTA TCATGGACAACTTTGAGTACCTGACTCGAGATTCGTCCATCCTGGGGCCTCACCACTTGGATGAATTTGT CCGTGTCTGGGCAGAGTATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTG ACTCTCATGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGTTGGTCC TGATGAATATGCCAGTGGCTGAGGACATGACAGTCCATTTCACCTCCACACTTATGGCTCTGATCCGGAC AGCTCTGGACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCAGAGCTACAAAAAGAG ACCCTGGCCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTAGTGCCCATGCCCAAAGCCTCTG ACCTGACTGTGGGCAAGATCTATGCAGCAATGATGATTATGGACTACTATAAACAGAGTAAGGTAAAGAA ACAGAGACAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCCTCACTGCCT CAGGAGATCATTTCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTC GGAGTGGATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGC GGATGATGGACAATTCCAGGAACAGCAATCGCTGGAGCCAGAGGTTAGTGAATTAAAAAGTGTGCAGTCC TCTAACCATGGCATCTACCTTCCTCCGGACACCCAGGAGCATGCGGGATCTGGGAGGGCATCCTCTATGC CACGTCTGACTATGGATCCCCAGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAATTCG GGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGTAGCAGTGAAAACACCTACAAG TCACGACGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCCCACCGCCTGAATTCTGACTCTGGTCACA AGTCTGACACTCACCGCTCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCATCTCCTCTCTCC GGATGTCTCCCGCTGCAATTCTGAGGAGCGAGGAACCCAGGCTGACTGGGAGTCCCCAGAACGTCGCCAG TCCAGGTCACCCAGTGAGGGAAGGTCACAAACCCCCAACAGACAGGGTACAGGTTCACTGAGTGAGAGCT CCATTCCCTCTATCTCCGACACCAGCACCCCAAGACGAAGTCGTCGCCAGCTCCCACCTGTGCCACCAAA GCCTCGGCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGATGGA AGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAAAGCAACAGCGCTTGCCTAACCGAGTCTTCCA ACTCCTTGCACCCCCAGCAGGGCCAGCACCCTTCCCCACAGCACTACATCTCTGAGCCCTATCTGGCCCT CCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCTGTAGCTACT AGCTTGGGCCGGTCCAATACCATTGGCTCTGCCCCACCCCTACGGCACAGCTGGCAGATGCCTAATGGGC ACTATCGGCGGCGGAGGCTGGGGGGCCTGGGCCTGGCCATGATGTGTGGAGCTGTCAGTGACCTCCTGAG TGACACGGAAGAAGACGATAAGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019294
Insert Size	6888 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019294.2, NP_062167.2
RefSeq Size	6973 bp
RefSeq ORF	6888 bp
Locus ID	54234
Gene Summary	T-type low voltage-activated calcium channel that may be involved in neuronal firing [RGD, Feb 2006]

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