Col7a1 (NM_001106858) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213650 representing NM_001106858 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCGGCTCCTGGTCGCTGCGCTCTGCACTGCCGAGATCCTGGTGGGGGCGCCCGGAGTGTGGG CCCAGCCCAGAGATAGAGTGACCTGCACCCGCCTTTATGCTGCTGACATTGTGTTCTTGATTGACGGCTC CTCGTCCATTGGCCGCAGCAACTTTCGAGAAGTGCGGGGATTCCTGGAGGGGCTGGTGCTGCCTTTCTCA GGAGCAGCCAATGCACAGGGCGTCCGATTTGCAACAGTGCAGTACAGTGATGATCCACAGACAGAATTTG GTCTGGATACACTTGGCTCTGGGGGTGACACCATTCGTGCCATCCGGGAGCTCAGCTATAAGGGTGGTAA CACTCGAACGGGGGCTGCTCTTCTCCACGTTTCCGACCGGGTCTTCCTGCCTCATCTAACACGTCCTGGC ATCCCCAAGGTCTGTATCCTGATCACAGATGGGAAATCCCAGGACTTAGTGGACACAGCTGCCCAAAAAC TGAAGAGGCAGGGGGTCAAGCTGTTTGCCGTGGGAATCAAGAACGCCGATCCTGAGGAACTGAAGCGCAT CGCCTCACAGCCAACCAGCGATTTCTTCTTCTTTGTCAATGACTTCAGCATCTTGAGGACCCTGTTGCCC CTCATTTCCCGGAGGGTGTGCACCACGGCTGGTGGTGTGCCTGTGACCCTGCCTTCCGATGACACTCCGT CTGGGCCCCGGGACCTGGTGCTGTCTGAGCCAAGTAGCCAATCCTTACGAGTACAGTGGACAGCAGCCAG TGGCCCTGTGACTGGCTACAAAATTCAGTACACGCCCCTGACGGGGCTAGGACAGCCACTGCCAAGTGAG CGAAAGGAGGTGAACGTCCCGGCTGGTGAGACCAGCATGCGACTACAGGGTCTCCGGCCACTAACTGACT ACCAAGTGACTGTGGTGGCCCTCTATGCTAACAGCATCGGAGAGGCTGTGAGTGGGACAGCTCGGACCAC TGCCAAGGAAGGGCTAGAGCTGAGCGTCCAGAACATCACATCCCACAGCCTCCTGGTGGCCTGGAGGAGG GTACCAGGTGCTAATGGCTACCGCGTAACATGGCGGGACCTCAGTGGTGGGGGGGCACAGCAGCAGGACC TGAGCCCTGGGCAGGGGTCAGTGTTTTTAGATCACTTGAAGCCTGGCACGGACTATGAGGTGACTGTGAG CGCCCTATTTGGCCGCAGCGTGGGGCCTGCTGCTTCTCTGACTACCCGCACTGCCTCTTCTGTTGAGCAG ACACTGCGCCCCATCATCCTGAGCCCCACATCTATTCTCCTTTCCTGGAACTTGGTGCCTGAGGCCCGCG GCTACCGCCTGGAGTGGCATCGTGAGAGTGGCTTGGAACCACCACAGAAAGTGGAACTGCTCCCTGATGT GACCCGCTACCAGTTGGATGGGTTGCAGCCAGGCACTGAGTATCGCCTCACGCTCTACACTCTGCTGGAG GGCCGTGAGGTGGCCACTCCTGCGACTGTGGTTCCTACTGGGCTAGAGCAGCTGGTGGGCCCGGTGATGA ATTTACAAGCCACAGAACTACCTGGGCAGCGGCTGCGAGTGTCTTGGAACCCAGTCCTCGGTGCCACCGA GTACCGTTTCACTGTACGCACCCCCCAGGGGGTCGAGCGGACTTTGTTGCTTCCTGGGAGTCAGACAACC TTTGACTTAGATGATGTTCGAGCCGGGATTAGCTACACAGTGCGAGTGTCTGCTCGAGTGGGCACTCATG AGGGTGATGCCAGCACCCTCACCATCCACAGAGATCAAGAAGCCCCACTTATTATCCCAGGACTGCAGGT TGTGGCATCAGATGCAACAAGAATTAGAGTGGCCTGGGGACTTGTCCCTGGAGCCAGTGGATTTCGGATT AGCTGGAGGACAGGCAGTGGTCCAGAGTCCAGTCAGACGCTGGCCCCAGACTCTACTGTCACAGACATCC TGGGGCTGCAGCCTGGAACCTCTTATCAAGTGGCTGTGTCAGCCCTTCGAGGGAGAGAAGAGGGGCCCCC TGTGGTCATCGTGGCTCGAACTGATCCACTGGGCCCAGTGAGGAGAGTCCACTTGACTCAGGCTGGTAGC TCATCTGTCAGCATTACCTGGACTGGGGTTCCTGGCGCCACCGGATACAGGGTTTCCTGGCACTCAGGCC ATGGTCCAGAGAAATCTCAATTGGTTTCTGGGGACGCCACGGTGGCTGAAATAGATGGACTGGAGCCAGA CACAGAGTATATAGTGCGTGTGAGAACCCATGTAGCCGGTGTGGATGGAGCCCCGGCCTCTGTGGTTGTG AGGACTGCTCCTGAGCCTGTAGGCAGTGTGTCAAAACTGCAAATCCTTAATGCTTCCAGTGATGTTCTCC GCGTCACTTGGGTGGGAGTCCCTGGAGCCACATCCTATAGACTGGCTTGGGGACGTAGTGAAGGTGGACC TATGAGACACCAGATAGTCCCAGGAAACAAAGACTCTGCAGAGATAACAGGTCTGGAAGGCGGAGTCAGC TACTCCGTGAGAGTGACGGCGCTTGTTGGAGACCGAGAGGGTGCACCCGTGTCCATTGTCGTCACCACAC CGCCTGAGGTCCCAGCACCTCTGGAGACCCTTCAGGTAGTGCAGAGTGGGGAGCACTCCTTGAGGCTGCA CTGGAACCGAGTCCCCGGAGCACAGGGCTTCCGTCTGCATTGGCAACCTGAGGGTGGCCAGGAACAGTCC CTGACCTTGGGACCTGAGTCTAACAGCTACAACCTGGTCGGACTGGAGCCAGCAACGAAGTACCATGTAT GGCTGAGTGTCCTGGGCCAGCCTGGAGAAGCTGCCCCTAGGAAGGTGACTGCTTACACTGAGACTCCTCA CATCCCGAGCACTGGGCTACGGGTGGTGGACACCTCTGTCAACTCAGTGACTTTGACCTGGACCCCAGTG TCTGGGGCATCCAGCTATATCTTGTCCTGGCGGCCACTCAGAGGAACTGGCCAGGAAGTGCCGGGGGGCC CGCAGACACTGCCAGGGAACTCAAGCTCCCACAGAGTGACAGGCCTGGAACCTGGGGTTCCCTATGTTTT CTCTCTGACACCTATCCAGAGCGGTGTACGAGGTTCTGAGATCACTGTCACACAAAAGCCAGCGTGTCCC CATGGCCAGGTGGACGTGGTGATCCTGTTACATGCCACTCGAGACAACGCTCACAATGCAGAAGCTGTAA AGAGGTTCCTGGAAAGGCTGGTGTCTGCACTCGGGCCTCTTGGTCCACAGGCTGCTCAGGTTGGCCTGCT GTCCTACAGCCACCGGCCCTCCCCACTGTTCCCGCTGAATAGTTCTCACGATCTTGGTGTCATCTTGCAA AAGATCCGTGACATCCCCTATGTGGACCCAAGTGGTAACAACCTAGGCACAGCCGTGACCACAGCTCACA GATACCTCTTAGCACCCAATGCTCCCGGTCGCCGTCGGCAAGTGCCAGGCGTGATGGTACTGTTAGTGGA TGAACCTCTGAGAGAAGACATATTGAGCCCCATTCGTGAGGCCCAGGCTTCTGGGCTCAAGGTGATGATG CTGGGTCTGGTGGGAGCTGACCCAGAGCAGCTGCGCCTCTTGGCACCAGGCATGGACCCCATCCAGACCT TCTTTGCTGTGGATAATGGCTTAGACTTGGACCGAGCAGGCAGTGATCTGGCTGTAGCCCTATGTCAGGC AGCTGTGGCCATACAGCCACAACTAGAGCCTTGTGCTGTGCCCTGTCCAAAGGGCCAGAAGGGGGAGCCT GGAGTGACGGGACCGCAAGGACAGGCTGGACCTCCTGGTCCCCCCGGTCTCCCGGGCAGGACCGGTGCTC CAGGCCCACAAGGTGCTCCCGGAAGTACCCAAGCAAAAGGCGAGAGGGGCTTTCCTGGCCCAGAAGGCCC TCCAGGCAGTCCTGGCCTACCTGGTGTTCCTGGGTCTCCTGGCGTTAAGGGCTCCCCAGGGTGGTCTGGC CCTCGTGGGGATCGGGGAGAACGAGGGCCTCAAGGCCCAAAAGGGGAGCCGGGGGAGCCTGGGCAAGTCA TTGGAGGCGGAAGGCCAGGGCTTCCTGGAAAGAAAGGGGACCCTGGACCTTCGGGACCCCCTGGACCTCA TGGCCCTTTGGGGGACCCAGGACCTCGTGGCCCCCCTGGGCTTCCTGGAACATCAGTGAAGGGTGACAAA GGTGACCGTGGGGAACGGGGTCCCCCAGGACCAGGCACTGGTGCCAGTGAGCAAGGCAGCCCTGGACTTC CGGGGCTTCCTGGAAGCCCTGGACCCCAAGGCCCACCTGGTCGAACTGGAGAGAAGGGAGAAAAGGGTGA CTGTGAGGATGGAGGCCCAGGCCTTCCAGGACAGCCTGGGGTTCCAGGGGAACCGGGCCTGCGGGGAGCT CCTGGAGTTACTGGGCCCAAAGGTGATCGGGGACTGACAGGGACCCCGGGTGAGCCTGGAGAGAAGGGTG AACGGGGACCACCTGGTCCCGTGGGACCCCAGGGGCTTCCCGGAGCTGCTGGACGTCCTGGAGTGGAGGG TCCTGAGGGGCCACCAGGACCCCCTGGCCGCCGGGGAGAAAAGGGAGAGCCTGGTCGCCCTGGGGATCCT GCATTGGGCCCTGGTGGTGCTGGAGCCAAGGGAGAAAAGGGAGATGCTGGGCTCCCTGGGCCCAGAGGAG CTTCTGGAATCAAAGGAGAGCAGGGTGCACCTGGGTTGGCTCTTCCTGGAGACCCTGGCCCCAAAGGAGA CCCTGGAGACAGAGGTCCCATTGGCCTCACTGGCAGGGCAGGACCCACAGGTGACTCAGGGCCTCCTGGA GAGAAGGGAGAACCTGGGCGACCTGGTTCCCCAGGACCTGTTGGCCCTCGAGGAAGAGATGGTGAAGCTG GAGAGAAAGGTGACGAGGGTGCCCCGGGTGAGCCAGGTTTGCCTGGAAAAGCAGGTGAACGCGGCCTCCG GGGGGCACCTGGGCCCCGGGGGCCTGTGGGTGAGAAGGGGAATGAGGGGGATCCTGGAGAAGATGGCCGG AATGGCACCCCTGGACCATCTGGACCCAAGGGTGACCGTGGGGAGCCGGGCCCCCCGGGTCTCCCTGGAC GGCTGGTGGATGCCGCCCTTGAATCCAGAGACAAGGGAGAGCCTGGACAGGAAGGTCCCCGAGGACCCAA GGGTGACCCTGGCCCCCCTGGAGCCTCTGGAGAAAGGGGCATTGATGGGCTTCGGGGACCCCCAGGCCCA CAGGGAGACCCAGGTGTTCGAGGTCCTGCAGGAGACAAGGGTGATCGGGGATCCCCAGGGCTGGATGGCC GAAATGGGCTAGATGGGAAACCAGGAGCCCCTGGCCCTCCAGGGCTACACGGTGCTTCAGGGAAAGCTGG GGACCCTGGGAGAGATGGACTTCCAGGCCTTCGAGGAGAACATGGCCCACCTGGTCCCCCTGGCCCTCCT GGAGTACCGGGAAAGCCAGGCGATGACGGCAAACCCGGCCTGAATGGGAAAAACGGAGAACCTGGGGATC CCGGAGAAGATGGAAGGAAGGGAGAAAAGGGAGATTCGGGTGTCCCTGGAAGGGAAGGTCCTGACGGTCC CAAGGGTGAACGCGGAGCTCCTGGTAATCCCGGACTCCAGGGTCCTCCGGGTCTCCCAGGGCAGGTCGGT CCTCCCGGCCAGGGTTTCCCTGGTGTCCCAGGAGTTACAGGTCCTAAGGGTGACCGTGGAGAGACTGGAT CCAAAGGGGAACAGGGCCTTCCTGGAGAACGTGGCCTACGAGGAGAACCGGGGAGCTTGCCGAATGCGGA GCGGTTCCTGGAAACTGCTGGCATCAAGGTGTCAGCCCTGCGGGAGATTGTGGACACCTGGGGTGAGAGT TCTGGCAGCTTCCTGCTGGTGCCTGAGCGGAGACAAGGTCCCAAGGGGGACCCTGGTGACCCAGGCCCTC CAGGCAAGGAGGGTTCCATTGGTCTTCCTGGAGAACGTGGGCTAAAGGGAGAACGAGGAGACCCTGGCCC TCAGGGGCCTCCTGGTCTCGCCCTTGGTGAGAGGGGCCCACCTGGTCCATCTGGCCTTGCCGGGGAACCA GGAAAGCCTGGAATTCCTGGGCTCCCAGGTCGGGCTGGGGCTGCAGGGGAAGCAGGAAGGCCAGGAGAGA GGGGAGAAAGGGGAGAGAAAGGAGAACGCGGGGAACAGGGCAGAGATGGCCATCCCGGCCTTCCTGGACC ACCTGGCCCTCCTGGCCCCAAGGTGGCCATTGAAGAGCTAGGTCCTGGACCCGCTAGAGAACAAGGACCT CCTGGACTCAAGGGTGCTAAGGGGGAGCCAGGCAGTGATGGTGTCCCAGGACCCAAAGGAGACAGGGGTG TACCCGGCATCAAAGGAGATGCAGGAGAGCCAGGAAAGAGGGGTCCTGATGGTAACCCAGGTCTGCCAGG AGAGCGCGGTGTGTCTGGACCTGAAGGGAAGCCGGGTCTGCAGGGCCCGAGGGGGACCCCTGGCCCAGTG GGTAGCCATGGAGACCCAGGACCACCTGGTGCCCCGGGTCTTGCTGGCCCTGCAGGGCCCCAGGGACCTT CTGGCCTGAAGGGGGAGCCCGGAGAGACAGGACCCCCAGGACGGGGTCTGCCTGGACCTACTGGAGCTGT GGGACTTCCTGGCCCTCCTGGCCCTTCAGGCCTCGTGGGCCCACAAGGCTCACCAGGTTTGCCCGGACAA GTGGGGGAGACAGGGAAGCCAGGACCCCCAGGCCGGGATGGTAGCAGCGGAAAGGACGGAGAAAGAGGAG GCCCTGGCGTGCCAGGGTTACCAGGTCTGCCCGGCCCTGTTGGACCTAAAGGAGAACCTGGACCTGTGGG GGCCCCTGGACAGGTGGTGGTCGGGCCCCCTGGAGCAAAAGGCGAGAAGGGAGCCCCAGGAGACCTTGCT GGAGCCCTGCTGGGTGAGCCGGGAGCCAAGGGTGACCGAGGACTGCCTGGACCACGGGGTGAAAAGGGTG AAGCTGGCCATGCAGGAGAGCCTGGCGACCCTGGGGAAGATGGTCAAAAAGGGGCTCCAGGACTCAAAGG TCTCAAGGGCGAGCCAGGCATTGGGGTTCAGGGCCCCCCTGGCCCAAGTGGTCCTCCAGGTATGAAGGGA GACTTGGGCCCCCCTGGTGCCCCCGGTGCTCCTGGTATTGTTGGATTTCCTGGTCAGCCAGGACCTCGAG GAGAGACGGGCCAGCCAGGGCCTGTTGGAGAGCGGGGTCTGGCAGGCCCTCCAGGGAGAGAAGGTGCCCC AGGTCCCTTGGGACCACCTGGACCACCGGGGTCAGTGGGAGCACCTGGCGCCTCTGGATTCAAAGGAGAC AAGGGAGACTCAGGAGCAGGGCTGCCAGGGCCCCGAGGCGAGCGTGGTGAACCAGGTCTCCGGGGTGAAG ATGGCCACCCTGGCCAGGAAGGACCTCGTGGACTCATGGGGCCCCCTGGCAGCCGGGGAGAGCGAGGAGA GAAGGGCGACCCTGGTGCTGCAGGACTCAAGGGTGACAAGGGTGACTCAGCTGTGATTGAGGGACCTGCA GGGCCACGAGGTGCCAAGGGGGATATGGGTGAACGAGGGCCTCGAGGCATAGATGGTGACCAAGGACCTC GGGGTGAAAGTGGGGATCCTGGGGACAAGGGCTCCAAAGGAGAGCCTGGAGACAAAGGCTCGGCTGGCTC CACTGGAGTTCGCGGACTCACAGGACCCAAGGCAAGGCGAGCCAGGTGCTGCAGGGATCCCCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106858
Insert Size	7767 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106858.1, NP_001100328.1
RefSeq Size	9170 bp
RefSeq ORF	7767 bp
Locus ID	301012

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