Fzd8 (NM_001044251) Rat Untagged Clone

CAT#: RN213682

Fzd8 (untagged ORF) - Rat frizzled homolog 8 (Drosophila) (Fzd8), (10 ug)



  "NM_001044251" in other vectors (3)

CNY 8,840.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC112790
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN213682 representing NM_001044251
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTGGGGTTACCTGTTGGAAGTGACCTCGCTACTAGCCGCCTTGGCGGTGCTACAGCGCTCGAGCG
GCGCTGCCGCGGCTTCGGCCAAGGAGCTGGCGTGCCAAGAGATCACAGTGCCGCTGTGCAAGGGCATCGG
TTACAACTACACCTACATGCCCAACCAGTTCAACCACGACACGCAAGATGAGGCGGGCCTAGAGGTACAC
CAGTTCTGGCCGCTGGTGGAGATCCAGTGCTCCCCGGACCTCAAGTTCTTTCTGTGTAGCATGTACACGC
CCATCTGCCTGGAGGACTACAAGAAACCTCTGCCGCCTTGCCGCTCTGTGTGTGAACGCGCCAAGGCCGG
CTGCGCGCCGCTCATGCGCCAGTACGGCTTTGCCTGGCCTGACCGAATGCGCTGCGATCGGCTGCCGGAG
CAGGGCAACCCCGACACTCTGTGCATGGACTACAACCGCACCGACCTCACCACGGCCGCGCCCAGCCCAC
CGCGCCGCCTGCCGCCGCCGCAGCCTGGCGAGCAGCCGCCCTCCGGCAGCGGCCACAGCCGCCCGCCAGG
GGCCAGGCCCCCACATCGTGGCGGCAGCAGTAGGGGCAGCGGGGACACGGCGGCTGCGCCCCCCTCTCGC
GGCGGGAAGGCGAGGCCCCCTGGTGGCGGCGCCGCTCCCTGCGAGCCCGGGTGCCAGTGCCGCGCGCCCA
TGGTGAGTGTGTCCAGCGAACGCCACCCGCTCTACAACCGCGTCAAGACCGGCCAGATCGCCAACTGTGC
GCTGCCCTGCCACAACCCCTTCTTTAGCCAGGATGAGCGCGCCTTCACTGTCTTCTGGATCGGCTTGTGG
TCGGTGCTCTGCTTCGTCTCCACCTTCGCCACTGTCTCTACCTTCCTCATCGATATGGAGCGCTTTAAGT
ACCCGGAACGGCCCATTATATTCCTCTCTGCCTGCTACCTCTTCGTGTCTGTCGGGTACCTGGTGCGCCT
GGTGGCTGGACACGAGAAAGTGGCCTGCAGCGGCGGCGCGCCGGGTGCAGGAGGAGCTGGGGGTGCTGGC
GGCGCGGCGACCGCTGGCGCGGGGGCGGCGGGAGCGGGGGCGAGCAGCCCGGGCGCGCGCGGCGAGTACG
AGGAGCTGGGTGCAGTGGAGCAGCACGTGCGCTATGAGACCACTGGCCCCGCGCTGTGCACGGTAGTCTT
CCTCCTTGTCTACTTTTTTGGCATGGCTAGCTCCATCTGGTGGGTAATCCTGTCTCTCACGTGGTTCTTG
GCGGCTGGTATGAAATGGGGCAACGAGGCCATAGCAGGCTACTCGCAATACTTCCACCTTGCCGCGTGGC
TCGTGCCCAGCGTCAAGTCCATAGCGGTGCTGGCGCTCAGCTCCGTGGACGGCGACCCGGTGGCGGGCAT
CTGCTACGTGGGCAACCAGAGCCTTGACAACCTACGCGGCTTTGTACTGGCGCCACTGGTCATCTACCTC
TTCATTGGGACTATGTTTCTGTTAGCTGGCTTCGTGTCGCTGTTCCGAATCCGCTCAGTCATCAAGCAAC
AAGGAGGCCCAACCAAGACTCACAAGCTAGAAAAGCTCATGATCCGCTTGGGCCTCTTCACCGTGCTCTA
CACGGTGCCCGCCGCCGTCGTTGTCGCCTGCCTTTTCTATGAGCAGCACAACCGACCGCGCTGGGAGGCC
ACGCACAACTGCCCATGCCTTCGCGACCTGCAGCCAGACCAGGCTCGCAGGCCCGACTACGCGGTCTTCA
TGCTCAAGTACTTCATGTGCCTAGTGGTGGGCATCACATCAGGCGTGTGGGTCTGGTCCGGCAAGACTCT
GGAGTCCTGGCGTGCGTTGTGTACTCGCTGCTGCTGGGCCAGCAAGGGCGCTGCAGTTGGCGCGGGCGCC
GGAGGCAGCGGCCCTGGGGGCGGTGGCCCCGGGCCCGGTGGGGGTGGGGGGCACGGCGGAGGTGGGGGAT
CCCTCTACAGCGACGTCAGCACCGGCCTGACGTGGCGGTCTGGCACGGCCAGCTCTGTATCTTACCCTAA
GCAAATGCCATTGTCCCAAGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001044251
Insert Size 2055 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001044251.2, NP_001037716.1
RefSeq Size 3012 bp
RefSeq ORF 2055 bp
Locus ID 364754
UniProt ID Q498S8
Gene Summary Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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