Apc (NM_012499) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RATAPC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213723 representing NM_012499 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGCCTCATATGATCAGTTGTTAAAGCAAGTTGAGGCCCTGAAGATGGAGAACTCAAATCTTC GACAAGAGCTCGAAGATAATTCCAATCACCTTACAGAACTGGAAACTGAGGCATCTAACATGAAGGAAGT ACTTAAGCAGCTGCAGGGAAGTATTGAAGATGAGACTATGACTTCCGGACAGATTGATTTACTAGAGCGC CTTAAAGAATTTAACTTAGATAGCAATTTCCCCGGAGTGAAACTACGCTCGAAAATGTCCCTCCGATCCT ATGGAAGTCGGGAAGGATCTGTATCAAGCCGTTCAGGAGAATGCAGTCCTGTCCCCATGGGGTCATTCCC AAGAAGAGCATTTGTAAATGGAAGCAGAGAAAGCACTGGATACTTAGAAGAGCTTGAGAAAGAGAGATCA TTACTCCTTGCTGATCTTGACAAAGAAGAAAAGGAAAAGGACTGGTATTATGCTCAGCTTCAGAACCTCA CGAAAAGAATAGATAGCCTGCCCTTGACTGAGAACTTTTCCTTACAGACAGACATGACAAGACGGCAGCT GGAGTATGAAGCACGGCAGATCAGGGCCGCAATGGAGGAGCAGCTTGGCACCTGCCAGGACATGGAGAAA CGAGCACAGCGAAGAATAGCCAGGATTCAGCAAATAGAGAAGGACATTCTTCGTGTACGGCAGCTCTTAC AGTCCCAAGCGGCTGAAGCAGAGAGGTCATCTCAGAGCAAGCATGAAACTGCCTCCCATGAAGCTGAGCG GCAGCTTGAAGGTCAAGGAGTGGCAGAAAGCAACTTGGCAACTTCTGGTAGTGGCCAGAGTTCAGCTGCA CGTGTGGATCATGAAACAGCCGGTGTTTTGAGTTCTAGCGGCACACACTCTGCTCCTCGAAGGCTGACGA GTCACCTGGGAACGAAGGTGGAAATGGTGTATTCATTGTTGTCCATGCTTGGTACTCATGATAAGGACGA TATGTCACGAACTTTGCTAGCTATGTCTAGCTCCCAAGACAGCTGTATATCCATGCGACAGTCTGGATGT CTTCCTCTCCTCATCCAGCTTTTACATGGCAATGACAAAGACTCTGTGTTGTTGGGAAATTCCCGGGGCA GTAAAGAGGCTCGGGCCAGGGCCAGTGCAGCCCTCCACAACATCATTCACTCACAGCCTGATGACAAGAG AGGCAGGCGTGAAATCCGGGTCCTTCATCTTTTGGAACAGATACGAGCTTACTGCGAAACCTGTTGGGAG TGGCAGGAAGCCCATGAACAAGGCATGGACCAGGACAAAAATCCAATGCCAGCTCCTGTTGAGCATCAGA TCTGCCCTGCGGTGTGTGTTCTGATGAAACTCTCATTTGATGAGGAGCACAGACATGCGATGAATGAGCT TGGGGGGCTGCAGGCTATTGCAGAGTTACTGCAGGTGGACTGTGAGATGCACGGGCTCACTGATGACCAC TACAGTGTCACTCTACGACGGTACGCTGGAATGGCCTTGACAAACCTGACCTTTGGAGATGTTGCCAACA AGGCTACGCTGTGTTCTATGAAAGGCTGCATGAGGGCACTCGTGGCCCAGTTAAAATCCGAAAGTGAAGA CTTACAGCAGGTTATTGCAAGTGTTTTGCGGAATTTGTCTTGGCGAGCAGATGTAAATAGCAAAAAGACG TTGAGAGAAGTTGGAAGTGTGAAAGCGTTGATGGAATGTGCTTTGGAAGTTAAAAAGGAATCAACTCTCA AAAGCGTCTTGAGTGCCTTATGGAATCTGTCCGCACACTGCACTGAGAATAAGGCTGACATATGTGCTGT GGACGGGGCACTGGCATTTCTGGTTGGCACCCTCACTTACCGGAGCCAGACAAATACGTTAGCCATCATT GAAAGTGGAGGTGGGATATTACGGAATGTGTCCAGCTTGATAGCTACCAACGAAGACCACAGACAAATCC TAAGAGAGAACAACTGCCTACAAACTTTATTACAGCACTTGAAATCTCACAGCTTGACAATAGTCAGTAA TGCATGTGGAACTCTGTGGAATCTCTCAGCGAGAAATCCTAAAGACCAGGAGGCTCTGTGGGACATGGGG GCGGTGAGCATGCTCAAGAACCTCATTCATTCCAAGCACAAAATGATCGCTATGGGGAGCGCAGCAGCTT TAAGGAATCTCATGGCAAACAGACCTGCAAAGTATAAGGATGCCAACATCATGTCTCCTGGCTCAAGTTT GCCATCCCTTCACGTTAGGAAACAAAAAGCTCTAGAAGCAGAATTAGACGCTCAGCATTTATCAGAAACC TTTGACAATATTGACAATTTAAGTCCCAAGGCATCTCATCGCAGTAAGCAGAGACACAAGCAGAATCTTT ATGGTGACTATGTCTTTGACGCCAGTCGACATGATGACAATAGGTCAGACAACTTTAACACTGGAAACAT GACTGTTCTTTCACCATACTTAAATACCACAGTATTGCCCAGCTCTTCTTCATCAAGGGGAAGTTTAGAC AGTTCTCGTTCTGAGAAAGACAGAAGTTTGGAGAGAGAACGAGGTATTGGCCTCAGCACTTACCATTCAG CAACAGAAAATCCGGGAACCTCTTCCAAACGGGGTTTGCAGCTCTCTGCCACTGCAGCCCAGATAGCCAA AGTGATGGAAGAAGTGTCCGCCCTCCACACCTCCCAGGACGACAGAAGCCCCGCCTCTGCCGCTGAGCTC CACTGTGTGGCGGAGGAGAGGACTGCAGCACGAAGAAGCTCTGCGTCCCACACACATCCAAACACACACA ACTTCGCTAAGTCGGAAAGCTCAAACAGGACATGCTCCATGCCTTATGCCAAGGTGGAATATAAGAGATC TTCAAATGACAGCTTAAACAGTGTCACTAGTAGTGATGGCTATGGTAAAAGGGGCCAGATGAAGCCCTCG GTTGAGTCCTACTCAGAAGATGATGAAGGTAAGTTTTGCAGTTATGGGCAGTATCCTGCCGACCTAGCCC ATAAGATACACAGTGCAAATCATATGGATGATAATGGTGGGGAGCTGGACACACCAATAAACTACAGCCT CAAGTACTCAGATGAGCAGCTGAACTCAGGAAGGCAGAGCCCCTCACAAAATGAAAGGTGGGCAAGACCC AAGCATGTGATAGAAGATGAAATAAAGCAAAACGAGCAAAGGCAATCAAGAAGCCAGAACACCAATTTTC CCGTCTACTCTGAGAACACTGATGACAAACACCTCAAGTTCCAACAGCATTTTGGGCAACAAGAGTGCGT TTCCCCATATAGGTCCAGGGGAACCAATGGTTCAGAAACAAATCGAATGGGTTCTAGTCATGCAGTTAAT CAAAATGTGAACCAGTCTTTGTGTCAGGAAGACGACTATGAAGATGACAAGCCTACCAACTACAGTGAAC GTTATTCTGAGGAAGAACAGCATGAAGAAGAAGAGAGACCGACAAATTACAGCATAAAGTATAATGAAGA GAAACACCATGTGGATCAGCCTATTGATTATAGTCTAAAATATGCCACTGACATATCTTCATCCCAGAAG CCATCATTTTCATTCTCAAAGACTCCATCAGTACAGGGCACTAAAACTGAACATAACTCTCCAAGCAGTG AGGCTGCATCTGCACCTTCATCTAACGCCAAAAGGCAGAGTCAGCTGCATCCAAGTTCAGCACAAAGAAA TGGTCAGACTCCAAAAGGGACTGCCTGCAAAGTCCCCTCCATCAACCAAGAGACAATGCAGACTTACTGT GTAGAAGACACCCCAATATGTTTCTCAAGGTGTAGTTCCTTATCATCGCTATCATCAGCTGAGGATGAAA TAGGCTGTGATCAGACAACACAGGAGGCAGACTCTGCGAATACTCTGCAAATAGCAGAGATAAAAGAGAA TGATGTGACGCGGTCAGCTCAAGACCCTGCGAGTGACGTCCCAGCCGTGTCCCAGAGTACTAGAACCAAG CCCAGCAGACTCCAGGCTTCCGGCTTAGCTTCAGAATCCGCCAGGCATAAAGCTGTTGAGTTTTCTTCAG GAGCCAAGTCTCCCTCCAAAAGCGGTGCTCAGACACCCAAAAGTCCCCCAGAACACTATGTCCAGGAGAC GCCCCTTGTATTCAGCAGGTGTACTTCTGTCAGCTCCCTGGACAGTTTTGAGAGTCGCTCCATCGCCAGC TCTGTTCAGAGTGAGCCATGTAGTGGAATGGTGAGTGGGATTGTCAGCCCCAGTGACCTTCCAGACAGCC CAGGGCAGACCATGCCACCAAGCAGAAGCAAGACCCCTCCCCCTCCCCCTCCCCCACAGCCAGTGCAGAC CAAGAGAGAGGTGCCAAAAACTAAGGTGCCTGCTGCCGAGCAGAGAGAGGGTGGGCCTAAGCAGACGGCC GTGAGTGCGGCTGTGCAGAGGGTGCAGGTCCTCCCGGATGCCGACACTCTGTTACACTTTGCCACAGAGA GTACTCCAGATGGGTTTTCTTGTTCCTCTAGCCTAAGTGCTCTGAGTCTGGATGAGCCCTTTATACAGAA AGATGTGGAATTAAGAATAATGCCTCCAGTTCAGGAAAACGACAATGGGAATGAAACTGAACCAGAACAG CCTGAAGAATCTAATGAAAACCAGGATAAAGAGGTAGAAAAGCCTGACTCTGAAAAAGACTTATTAGATG ATTCCGATGATGATGATATTGAAATATTAGAAGAATGTATTATTTCAGCCATGCCAACAAAGTCATCACG CAAAGCCAAAAAGCTAGCCCAGACTGCTTCAAAATTACCTCCTCCTGTGGCAAGGAAACCGAGTCAGCTG CCTGTGTACAAACTTCTGCCGTCACAGAGCAGGCTGCAGGCACAAAAACATGTCAGCTTTACACCAGGGG ACGATGTGCCCCGAGTGTACTGTGTGGAAGGGACGCCTATAAACTTCTCCACAGCAACATCTCTGAGTGA TCTCACAATAGAATCCCCTCCGAATGAGCTGGCTGCTGGAGATGGGGTTAGAGCAAGTGTACAGTCAGGT GAGTTTGAAAAACGAGATACCATTCCTACAGAGGGCAGAAGTACAGATGAGGCTCAGAGAGGAAAAGTCT CCTCTATAGCTATACCAGACCTGGATGGTAGCAAAGCAGAGGAAGGAGATATTCTTGCAGAATGCATCAA TTCTGCCCTGCCCAAAGGAAGAAGCCACAAGCCTTTCCGAGTGAAAAAGATAATGGACCAAGTCCAACAG GCATCCATGACTTCATCCGGAACTAACAAAAATCAAATAGACACTAAGAAAAAGAAGCCTACTTCACCAG TAAAGCCCATGCCACAAAATACTGAATACAGAACTCGTGTGAGAAAGAATACAGACTCAAAAGTTAACGT AAATACTGAAGAAACTTTCTCAGACAATAAAGACTCAAAGAAACAGAGCTTAAAAAACAACCCCAAGGAC TTGAATGACAAGCTACCGGACAATGAGGACAGGGTCCGGGGAGGCTTTACTTTCGATTCACCGCATCATT ACGCACCCATTGAAGGGACTCCTTACTGCTTCTCACGAAATGACTCTTTGAGTTCTCTAGATTTTGATGA TGACGATGTTGACCTCTCCAGGGAAAAGGCTGAGTTAAGAAAGGGCAAAGAAAGTAAGGACTCCGAAGCC AAAGTTACCTGCCACACAGAACCAAGCTCAAGCCAACAGTCGGCTAGGAAGGCACAGGCTAGTACAAAAC ATCCAGTGAACAGAGGGCCGTCTAAACCACTGCTGCAGGAACAACCCACTTTCCCCCAGTCCTCTAAAGA CGTACCAGACAGAGGGGCAGCAACTGACGAAAAACTGCAGAATTTTGCTATTGAAAATACACCGGTTTGC TTTTCTCGAAATTCTTCTCTGAGCTCCCTTAGTGACGTTGACCAAGAAAACAACAATAACGAAGAAACTG GACCAGTCAGAGACGCTGAGCCTGCCAACGCGCAAGGACAGCCAGGCAAGCCTCAGGCATCTGGGTATGC GCCCAAGTCCTTTCATGTGGAAGACACCCCTGTGTGCTTCTCCAGAAACAGCTCTCTCAGCTCTCTGAGC ATTGATTCTGAGGACGACCTGTTGCGAGAGTGCATAAGCTCTGCTATGCCAAAAAAGAGGAGGCCTTCCA GACTCAAGGGTGAGGGTGAGTGGCAGAGTCCTAGGAAAGTGGGCAGCGTGTTAGCCGAAGATCTGACACT CGACTTGAAAGATATACAGAGGCCAGAGTCAGAACACGGTTTATCCCCCGATTCAGAAAATTTCGACTGG AAAGCTATTCAGGAAGGTGCAAACTCCATAGTAAGTAGTCTGCACCAAGCTGCCGCCGCCGCCGCGTGTC TGTCCAGACAAGCGTCGTCGGACTCAGACTCCATCCTGTCCCTGAAGTCGGGCGTCTCCCTGGGGTCGCC TTTTCACCTTACACCTGACCAAGAGGAGAAGCCATTCACAAGTCATAAAGGCCCAAGAATTCTCAAACCT GGGGAGAAAAGCACATTAGAAGCAAAAAAAATAGAATCCGAAAACAAAGGAATCAAAGGTGGAAAAAAGG TTTATAAAAGCTTGATTACAGGAAAGATTCGATCTAATTCAGAAATCTCCAGCCAAATGAAACAACCCCT TCAGACAAACATGCCTTCAATCTCAAGAGGCAGGACAATGATTCACATCCCGGGAGTTCGGAACAGCTCC TCAAGTACAAGCCCTGTCTCTAAAAAAGGCCCACCCCTCAAGACTCCAGCCTCTAAAAGCCCCAGTGAAG GTCCTGTAGCTACCACTTCTCCTCGTGGAACTAAGCCAGCAGTGAAGTCAGAGCTGAGCCCTATTACCAG GCAAACTTCCCACATTAGTGGGTCAAATAAGGGGCCCTCTAGGTCAGGGTCTAGAGACTCCACTCCCTCG AGACCCACACAGCAGCCATTAAGTAGGCCGATGCAGTCGCCAGGGCGGAACTCGATCTCCCCCGGTAGAA ATGGAATAAGCACTCCTAACAAACTCTCTCAGCTGCCCAGAACATCGTCTCCCAGTACTGCTTCGACCAA GTCCTCGGGGTCGGGGAAAATGTCCTACACATCCCCAGGCCGGCAGCTGAGCCAGCAAAACCTCAGCAAA CAAACAGGCTTGTCCAAGAACGCCAGCAGCATCCCCAGAAGTGAGTCAGCATCCAAAGGACTGAATCAGA TGAATAACAGCAATGGGTCTAATAAAAAGGTAGAACTTTCTAGAATGTCTTCAACTAAGTCAAGTGGAAG TGAATCAGACAGGTCAGAAAGACCTGCATTAGTACGCCAGTCCACTTTCATCAAAGAAGCCCCAAGCCCA ACCCTAAGGAGGAAACTGGAGGAATCTGCCTCATTTGAATCCCTTTCTCCATCTTCTAGACCAGATTCTC CCACCAGGTCCCAGGCACAGACCCCAGTTTTAAGCCCTTCCCTTCCCGATATGTCTCTGTCCACACATCC ATCTGTTCAGGCAGGTGGATGGCGCAAGCTCCCACCTAACCTCAGCCCCACTATAGAGTACAGTGACGGA AGGCCCTCAAAGCGGCATGATATCGCACGCTCCCATTCTGAAAGTCCGTCCAGACTACCAGTCAACCGAG CGGGAACCTGGAAGCGTGAACACAGCAAACATTCCTCATCCCTTCCTCGAGTGAGCACTTGGAGAAGAAC TGGAAGCTCATCTTCTATTCTTTCTGCTTCTTCAGAGTCCAGTGAAAAAGCAAAAAGTGAGGATGAAAAG CATGTGAACTCTGTGCCAGGACCCAGACAAATGAAAGAAAACCAGGTACCCACAAAAGGAACATGGAGGA AAATAAAGGAAAGTGAAATTTCTCCCACAAACACGGTTTCTCAGACCACTTCCTCAGGTGCTGCCAGTGG TGCTGAATCAAAGACTCTGATCTATCAGATGGCACCTGCTGTTTCTAGAACAGAGGACGTTTGGGTGAGA ATTGAGGACTGCCCCATTAACAACCCTAGATCTGGAAGATCTCCCACAGGTAACACCCCCCCAGTGATTG ACAGCATTTCAGAAAAGGGAAATCCAAGCATTAAAGATTCAAAAGACACCCAAGGAAAACAAAGTGTGGG CAGTGGCAGTCCTGTGCAAACCGTGGGTCTAGAAAACCGCCTGAACTCCTTTATTCAGGTAGAGGCCCCG GAACAGAAAGGAACTGAGACTAAAGCAGGACAGGGTAGCCCTGCCCCTGTAGCAGAGACTGGTGAGACCT GCATGGCAGAGCGCACCCCTTTCAGTTCAAGTAGCTCCAGCAAGCACAGTTCTCCCAGTGGGACGGTCGC TGCCAGAGTGACGCCTTTTAATTACAACCCAAGCCCTAGGAAAAGCAGCGCAGACAGCACTTCAGCCCGG CCATCTCAGATCCCCACTCCGGTGGGCAGCAGCACAAAGAAGAGAGACTCGAAGACTGACAGCACTGAAT CTAGTGGAGCCCAGAGTCCTAAACGCCATTCTGGGTCTTACCTCGTGACGTCTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012499
Insert Size	8529 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012499.1, NP_036631.1
RefSeq Size	8582 bp
RefSeq ORF	8529 bp
Locus ID	24205
UniProt ID	P70478
Gene Summary	binds microtubules; may play a role in the regulation of cell polarity [RGD, Feb 2006]

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