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Gpr183 (NM_001109386) Rat Untagged Clone
CAT＃: RN213725
Gpr183 (untagged ORF) - Rat G protein-coupled receptor 183 (Gpr183), (10 ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ebi2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN213725 representing NM_001109386
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAACAATTTTACTACACCACTGGCAGCTTCTCACGGCAATAACTGTGATCTCTATGCACACCACA GCACGGCCAGGATATTAATGCCTCTGCATTATAGCCTGGTCTTCATCATTGGTCTGGTGGGAAACTTGTT GGCCTTAGTTGTCATTGTTCAAAATAGAAAAAAAATCAACTCAACCACTCTCTATTCAATGAACTTGGTG ATTTCCGACATCCTGTTTACCACAGCTTTACCTACGCGGATAGTCTACTATGCCCTGGGTTTTGACTGGA GGATTGGTGATGCCCTGTGCCGGATAACTGCTCTGTTGTTCTACATCAACACGTATGCAGGTGTGAACTT CATGACTTGTTTGAGCATAGACCGATTCTTTGCCGTGGTGCACCCTCTGCGCTACAACAAGATTAAAAGA ATTGAATATGCAAAGGGCATTTGTGTATTTGTCTGGATTTTGGTGTTTGCTCAAACACTGCCACTGCTCC TCAAACCCATGTCTAAGCAGGAGGCAGACAAGACTACTTGCATGGAATATCCAAATTTTGAAGGGACAGC ATCTCTCCCGTGGATTCTGCTCGGAGCCTGCCTGCTAGGCTACGTGCTGCCTCTTGCCATCATTCTCCTG TGCTACTCTCAGATCTGCTGCAAACTCTTCAGGACTGCCAAGCAGAACCCACTGACCGAGAAATCTGGTG TGAACAAAAAGGCTCTCAACACGATCATCCTCATCATCGGCGTGTTCGTCTTGTGCTTCACGCCCTACCA CGTGGCCATTATGCAGCACATGGTGAAGACTCTCTACGCCCCCGGAGCCCTCGGCTGTGGGGTGAGACAC TCCTTCCAGATCTCTCTGCACTTCACAGTGTGCCTGATGAACTTCAACTGCTGCATGGACCCTTTCATAT ACTTCTTTGCATGTAAAGGGTACAAGAGAAAGGTCATGAAGATGCTCAAACGTCAAGTGAGCGTGTCAAT CTCCAGTGCAGTGAGGTCAGCCCCTGAGGAGAACTCACGGGAAATGACGGAGTCACAGATGATGATCCAC TCCAAGGCCTCCAATGGGAGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001109386 |
| Insert Size | 1074 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001109386.1, NP_001102856.1 |
| RefSeq Size | 1536 bp |
| RefSeq ORF | 1074 bp |
| Locus ID | 679975 |
| UniProt ID | D4A7K7 |
| Gene Summary | G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity). Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (By similarity). Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity). Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity). Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity). Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (By similarity). Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity). Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity). Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels (By similarity). Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (By similarity). Promotes osteoclast precursor migration to bone surfaces (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (By similarity). Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR213725 | Gpr183 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 183 (Gpr183), (10 ug) |
CNY 5,856.00 |
|
| RR213725L3 | Lenti ORF clone of Gpr183 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 183 (Gpr183), (10 ug) |
CNY 6,080.00 |
|
| RR213725L4 | Lenti ORF clone of Gpr183 (mGFP-tagged ORF) - Rat G protein-coupled receptor 183 (Gpr183), (10 ug) |
CNY 6,650.00 |
