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P3h2 (NM_001025627) Rat Untagged Clone
CAT＃: RN214000
Leprel1 (untagged ORF) - Rat leprecan-like 1 (Leprel1), (10 ug)
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Leprel1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214000 representing NM_001025627
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGAGAGCACGTGGGTGTCGCTGCTGTTGCTGCTGCTGCTGCCCGCGCCACAGCGGGGCGGCCCCC AGGACGGTCGCGGGTCGCCGGAGCCGGAGCCAGAGCGCGGCCCGCTGCAGCCCTTCGACCTGCTCTACGC TAGCGGCGTGGCTGCCTACTACAGCGGCGACTACGAAGGTGCGGTGCGGGACCTAGAAGCCGCGCTGCGC AGCCACCGGCGCCTGCGAGACATCCGCACGCGCTGCGCCCGCCACTGCGCCGCGCGCCGCCCGCTCGCGC CCCCAGGCGCCGGCCCGGGAGCCGAGCTGCCCTTCTTCCGCGCGGTGCTCGAACGAGCGCGCTGCTCCCG CAGCTGCCAGAGCCAGCGTCTGGGCGGCCCCGCGTCCCGCCACCGTGTCAGCGAAGACGTGCGCAGCGAC TTCCAGCGCAGAGTGCCCTACAACTACCTGCAGAGGGCCTACATCAAGCTCAACCAGCTCGATAAAGCCA TGGAAGCAGCTCACACATTCTTCATGGCCAACCCCGAGCACATGGAAATGCAGCAGAACATAGAAGACTA TAAAGCCACAGCAAGAGTGGAAGCCCCGTTAGTAGACAGAGAAGCCAAGCCACACCTGGAAAGCTATAAT GCTGGAGTTAAACATTATGAGGCGGATGACTTTGAAGCAGCCATCAAGTACTTTGAACAAGCTTTAAGAG AATATTTCAATGAAGATATGGTGTGCCGCGCTCTGTGTGAGGGGCCTCAGAGATTTGAGGAATATGAGTA TTTAGGATCTAAGGGCAGTCTTTATGAGGCAATCGCAGATCACTACATGCAGGTTCTGGTCTGTCAGCAT GAGTGTGTGAGGGAACTTGCCACCCGCCCAGGCCGCCTCTCACCAATCGAGAACTTTCTTCCCTTGCATT ATGACTACCTCCAGTTTGCCTACTACAGAGTTGGTGAGTATGTGAAAGCCTTGGAGTGTGCCAAAGCCTA CCTGATGTTCCACCCAGACGACCAGGATGTCCTGGACAATGTAGACTTCTATGAGAGTCTGCTGGATGAC AGCACTGACCCAGCATCCATTGAGGCCCGAGAGGATTTAACAGCATTTGTCAAACGTCATAAACTTGAAG CTGAACTGATCAAGTCAGCTGCAGAGGGCCTGGGATTTTCATATTCTGAACCAAATTACTGGATCAGTTA TGGAGGACGGCAGGATGAGAACCGAGTCCCTTCGGGAGTGAATATGGATGGGGCAGAAGTTCACGGATTG TCCATGGGGAAGAAGTCACCACCCAAGATAGGTCGAGATCTTAGAGAAGGTGGCCCTCTGCTCTATGAGA ACATCACCTTCGTCTACAACTCCGAGCAGCTGAACGGGACTCAGCGGGTTCTCCTAGATAACGTCCTGTC GGAAGAACAGTGCCGGGAGCTGCACAGTGTGGCCAGTGGAATCATGCTGGTTGGTGATGGATACCGAGGG AAAACGTCACCCCACACACCCAATGAGAAGTTTGAAGGAGCAACTGTTTTGAAAGCACTCAAATTTGGTT ATGAAGGCCGGGTGCCACTGAAGAGTGCACGGCTGTTCTATGACATCAGCGAGAAGGCTCGGAAGATTGT GGAGTCCTATTTCATGCTGAACTCAACCCTGTATTTTTCCTATACACACATGGTCTGCAGAACAGCCCTG TCTGGGCAGCAGGATCGAAGAAATGACCTCAGTCACCCCATTCATGCGGACAACTGCTTGCTGGATCCAG AAGCCAACGAATGCTGGAAGGAGCCTCCTGCTTACACCTTTCGGGACTATAGTGCTCTCCTGTATATGAA TGACGACTTTGAAGGAGGAGAATTCATCTTCACGGAAATGGATGCTAAAACTGTGACTGCCTCTATAAAG CCAAAATGTGGGCGAATGATCAGCTTCTCATCTGGTGGCGAGAACCCTCACGGGGTGAAGGCAGTCACCA GAGGCCAGCGCTGTGCTGTGGCACTGTGGTTCACCCTAGACCCTCTCTACCGAGAATTGGAGCGGATACA GGCTGATGAAGTGATTGCCATCTTGGATCAAGAACAACACGGGAAGCATGGACTGAATATTAACCCCAAA GACGAGCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025627 |
| Insert Size | 2112 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025627.1, NP_001020798.1 |
| RefSeq Size | 2387 bp |
| RefSeq ORF | 2112 bp |
| Locus ID | 288016 |
| UniProt ID | Q4KLM6 |
| Gene Summary | Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed:21757687). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (By similarity). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that lack 4-hydroxyproline in the third position (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214000 | Leprel1 (Myc-DDK-tagged ORF) - Rat leprecan-like 1 (Leprel1), (10 ug) |
CNY 9,030.00 |
|
| RR214000L3 | Lenti ORF clone of Leprel1 (Myc-DDK-tagged ORF) - Rat leprecan-like 1 (Leprel1), (10 ug) |
CNY 10,830.00 |
|
| RR214000L4 | Lenti ORF clone of Leprel1 (mGFP-tagged ORF) - Rat leprecan-like 1 (Leprel1), (10 ug) |
CNY 11,970.00 |
