Ciao1 (NM_001008766) Rat Untagged Clone

CAT#: RN214206

Ciao1 (untagged ORF) - Rat cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae) (Ciao1), (10 ug)



  "NM_001008766" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Wdr39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214206 representing NM_001008766
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGATGCGCTGGTACTGCAGAGCCGCGTCCCGGCGCACCCGGATTCCCGCTGCTGGTTCCTGGCCT
GGAACCCCACGGGAACCTTGTTGGCCTCATGCGGAGGCGACCGTAAAATTCGCATCTGGGGCACCGAGGG
TGACAGCTGGATTTGCAAGTCTGTCCTTTCTGAAGGCCATCAGCGCACCGTTAGGAAAGTGGCCTGGTCC
CCCTGTGGAAATTACCTGGCATCTGCCAGCTTTGATGCTACTACTTGCATTTGGAAGAAGAACCAGGATG
ACTTTGAGTGTGTGACCACTCTTGAGGGTCATGAGAATGAGGTCAAGTCAGTGGCTTGGGCCCCATCTGG
CAACCTCTTGGCTACCTGCAGCAGAGATAAGAGCGTGTGGGTCTGGGAAGTTGATGAAGAAGATGAGTAT
GAATGCGTCAGTGTCCTCAATTCTCACACACAGGATGTTAAGCATGTGGTTTGGCACCCAAGCCAGGAGC
TTTTAGCTTCTGCTAGCTATGATGACACAGTGAAGCTATACCAGGAAGAAGGGGATGACTGGGTCTGCTG
TGCTACCCTTGAAGGTCACGAGTCCACTGTATGGAGCATAGCCTTTGATCCCAGCGGCCAGCGTCTGGCA
TCTTGCAGTGATGACCGAACTGTGCGCATCTGGCGCCAGTATCTACCTGGCAATGAACAAGGGGTGGCAT
GCAGTGGCTCCGACCCCAGCTGGAAATGTGTTTGCACTTTGTCGGGCTTCCATACCAGGACCATCTACGA
CGTTGCTTGGTGTCAGCTGACAGGGGCCCTGGCTACAGCTTGTGGAGATGATGCCATCCGAGTGTTTGAG
GAAGATCCAGGCTCAGACCCACAGCAGCCCACCTTCTCTTTGACAGCCCATCTTCGTCAGGCCCATTCCC
AGGATGTCAACTGTGTGGCTTGGAACCCCAAGGAGGCAGGGCTCCTGGCCTCTTGTAGTGATGATGGGGA
GGTAGCCTTCTGGGAGTATCATCAGCCTGCAGGTCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001008766
Insert Size 1020 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001008766.1, NP_001008766.1
RefSeq Size 1458 bp
RefSeq ORF 1020 bp
Locus ID 29231
UniProt ID Q5M7T1
Gene Summary Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (By similarity). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (By similarity). Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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