Plcg1 (NM_013187) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	PPLCA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214485 representing NM_013187 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTCGGGACCCCCTGCGCCAACGGCTGCGGGCCCAGCGCGCCCTCCGAAGCGGAGGTGCTGC ACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGGCCAGA ACGGAAGACCTTCCAGGTCAAGTTGGAGACGCGCCAGATCACATGGAGCCGCGGTGCGGACAAAATCGAG GGGTCCATCGATATCCGAGAAATCAAGGAGATCCGCCCAGGGAAGACTTCCCGGGACTTTGACCGCTACC AAGAAGACCCTGCCTTCCGGCCAGATCAGTCACACTGTTTTGTCATCCTCTATGGAATGGAATTCCGCCT GAAGACCCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTC ATGGAAGATACGCTGCAGGCGGCCACACCCCTGCAAATTGAGAGATGGCTCCGGAAGCAGTTCTACTCAG TGGATCGTAACCGAGAGGATCGTATATCAGCCAAGGACCTGAAGAACATGCTGTCACAGGTCAACTACCG GGTCCCCAACATGCGCTTCCTCCGAGAGCGGCTGACGGACTTTGAACAGCGCAGCGGGGACATCACCTAT GGGCAGTTTGCTCAGCTTTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTTCCGTTCTTGG AAACCAACACTTTGAGGACTGGAGAGCGGCCAGAGCTTTGCCAGGTGTCCCTTTCTGAGTTCCAGCAGTT CCTTCTTGAGTACCAGGGGGAGCTGTGGGCTGTCGACCGGCTTCAGGTGCAGGAATTTATGCTCAGCTTC CTTCGAGACCCCTTGCGAGAGATTGAGGAGCCATACTTCTTCTTGGATGAGCTTGTCACCTTTCTGTTCT CCAAAGAGAACAGTGTGTGGAACTCACAGCTGGATGCCGTGTGCCCAGAAACCATGAACAACCCACTGTC TCACTATTGGATCTCTTCCTCGCATAATACGTATCTGACTGGGGACCAGTTCTCCAGCGAGTCCTCCCTG GAAGCCTACGCTCGCTGCCTGAGGATGGGCTGTCGCTGCATCGAGTTGGACTGCTGGGATGGGCCAGATG GGATGCCAGTCATTTACCATGGGCACACCCTCACCACCAAGATTAAGTTCTCAGATGTCCTGCACACCAT CAAGGAGCACGCGTTCGTAGCCTCAGAGTACCCTGTCATCCTGTCCATCGAGGACCACTGCAGCATTGCC CAGCAGAGGAACATGGCCCAGCACTTCAGGAAGGTGCTCGGTGACACGCTCCTCACCAAGCCCGTGGACA TTGCCGCTGATGGGCTCCCTTCTCCCAACCAGCTCAAGAGGAAGATCCTGATTAAGCATAAGAAGCTGGC TGAGGGCAGTGCCTATGAGGAGGTGCCTACCTCTGTGATGTACTCTGAGAATGACATCAGTAACTCCATC AAGAATGGTATCCTCTACTTGGAGGACCCCGTGAATCATGAGTGGTACCCCCACTACTTTGTTCTGACTA GCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGAAATGAGGATGAAGAGGAGCCGAAGGA GGCCAGTGGCAGCACAGAGCTGCACTCGAGCGAGAAGTGGTTCCACGGGAAGCTCGGGGCTGGGCGCGAC GGGCGGCACATTGCTGAGCGCCTGCTCACCGAGTACTGCATAGAGACTGGGGCTCCCGATGGCTCCTTCC TAGTGCGAGAAAGTGAGACCTTCGTGGGGGACTACACGCTGTCTTTTTGGCGGAATGGGAAAGTCCAGCA CTGCCGTATCCACTCCCGGCAGGATGCTGGGACTCCTAAGTTCTTCTTGACAGATAACCTTGTCTTTGAC TCTCTCTATGACCTCATCACACATTATCAGCAAGTGCCCCTGCGCTGCAATGAGTTTGAGATGCGCCTTT CAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCA GGCTGAACACATGCTGATGCGAGTACCCCGTGATGGGGCCTTCCTGGTGCGGAAGCGCAACGAGCCCAAC TCCTATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACTG TGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTCGACCTCATCAGCTACTATGAGAAGCATCCCCTGTA CCGCAAAATGAAACTGCGCTACCCCATCAACGAGGAGGCGCTGGAGAAGATTGGGACAGCTGAACCCGAT TATGGGGCACTGTATGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCCAACCCTATGCCAACTTTCAAGT GTGCAGTAAAAGCTCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACTTTTACCAAGAGCGCCAT CATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGTGGGGACTATGGTGGGAAGAAGCAGCTGTGG TTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAATCCTAGAGCCGGAGAGGGAGCATCTGGATG AGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCCTGCCAGATCGCCATTCGTCC TGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCGTCAGTGGCTCAGTGGTCCCTAGAC GTTGCCGCTGACTCACAGGAGGAGTTGCAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAG ATGCCAGGCTTACTGAGGGGAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTCGT GGTCTACTGCCGGCCTGTTCCCTTTGACGAAGAGAAGATTGGCACAGAACGCGCTTGTTACCGGGACATG TCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACA ACCGGCTGCAGCTCTCTCGCATCTACCCTAAGGGTCAGAGGCTGGACTCCTCCAATTATGACCCTCTGCC CATGTGGATCTGTGGCAGCCAGCTTGTAGCTCTCAATTTTCAGACCCCAGACAAGCCTATGCAGATGAAC CAGGCCCTCTTCATGGCTGGTGGACACTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGATGAAGCCT TTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAGCCCTGTGTCATTTGCATTGAGGTGCTGGGGGC CAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTCGTGGAGATTGAAGTGGCTGGGGCAGAG TACGACAGCACCAAGCAGAAGACAGAGTTTGTAGTGGACAATGGACTGAACCCTGTGTGGCCTGCAAAGC CCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTT TAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATACAGAGCAGTG CCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTG CTAAGGAGAATGGTGACCTCAGCCCTTTCAGTGGTACATCCCTAAGGGAACGGGCCTCAGATGCCTCCAG CCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGACTTC CGCATCTCGCAGGAGCATCTCGCAGACCATTTTGACAGTCGGGAACGAAGGGCCCCAAGAAGGACTCGGG TCAATGGAGACAACCGCCTCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_013187
Insert Size	3873 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013187.1, NP_037319.1
RefSeq Size	5106 bp
RefSeq ORF	3873 bp
Locus ID	25738
UniProt ID	P10686
Gene Summary	mediates intracellular agonist-induced Ca2+ entry (ACE); serves as a guanine nucleotide exchange factor for the nuclear GTPase PIKE [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.