Col2a1 (NM_012929) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CG2A1A; COLLII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214632 representing NM_012929 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCACGGTCCTACAATGTC AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAAAGATATCAT AGGACCTAAAGGACCTCCTGGCCCTCAGGGACCTGCCGGTGAACAAGGACCCAGAGGTGACCGTGGTGAC AAGGGAGAGAGGGGTGCACCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGTC CCCCTGGCCCTCCAGGCCCCCCTGGTCCCCCTGGCCTTGGTGGAGGAAACTTTGCAGCCCAGATGGCTGG AGGATTTGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGCCCCATGGGCCCCATGGGACCT CGTGGACCCCCAGGCCCCGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG AGCCTGGTGTCTCTGGTCCCATTGGTCCCCGAGGTCCTCCAGGTCCTGCTGGAAAACCTGGTGATGATGG TGAAGCTGGAAAGCCCGGAAAGGCCGGAGAAAGAGGCCTCCCTGGTCCTCAGGGTGCTCGTGGATTCCCG GGAACCCCCGGTCTTCCTGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGATGGTGCTAAGGGAGAAG CTGGTGCTCCAGGTGTGAAGGGTGAGAGCGGTTCCCCTGGTGAGAACGGATCCCCAGGCCCAATGGGTCC CCGTGGCCTGCCTGGCGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGAGGCAACGACGGC CAGCCAGGCCCTGCTGGACCTCCGGGTCCTGTTGGTCCTGCAGGTGGTCCTGGCTTCCTTGGTGCTCCTG GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAACC CGGCAATCCCGGATCCCCCGGGCCCGCAGGCGCTTCTGGTAACCCAGGGACTGATGGTATTCCCGGAGCC AAAGGATCTGCTGGCGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC CTCAAGGTGCAACTGGTCCTCTGGGCCCCAAAGGTCAGACGGGTGAGCCCGGCATCGCTGGCTTCAAAGG TGAACAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAGGGAGCCCCTGGCCCTGCTGGTGAAGAA GGAAAACGAGGTGCTCGAGGAGAGCCCGGTGGCGCTGGACCTATCGGCCCCCCTGGAGAGAGAGGTGCTC CTGGCAACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC TAGTGGCCTGGCTGGTCCTAAGGGTGCCAATGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA GCCAGGGGTCTTACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG GAGAAGACGGTCGCCCTGGTCCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC TGGCCCCAAAGGAGCCAATGGCGAGCCTGGCAAAGCTGGTGAGAAAGGACTGGCTGGTGCTCCTGGTCTG AGAGGTCTTCCTGGCAAAGATGGTGAGACAGGAGCCGCAGGACCCCCCGGCCCCAGCGGACCTGCTGGTG AACGAGGCGAGCAGGGTGCTCCTGGGCCATCAGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCGGG TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCCCCTGGCCTCGTTGGTCCCCGG GGTGAACGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCTCAGGGCCTCCAGGGTCCCCGAGGCCTCC CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCGGCTGGCCCAGATGGTCCCCCTGGGGCTCAGGGCCC TCCAGGTCTACAGGGAATGCCTGGTGAGAGGGGAGCAGCTGGTATTGCTGGACCCAAGGGAGACAGAGGT GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGAAAAGATGGTGGCCGAGGTCTTACTGGGCCCATCG GGCCCCCAGGACCAGCAGGTGCCAATGGCGAGAAGGGAGAAGTCGGACCTCCTGGTCCTTCAGGAAGTAC TGGAGCTCGAGGCGCCCCGGGTGAGCGCGGAGAGACTGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGCGATCAAGGAGAAGCTGGACAGAAAGGTGACGCTGGTG CCCCTGGCCCACAAGGCCCCTCAGGAGCTCCCGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG AGCCCGGGGCGCCCAAGGCCCACCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA GGTTCTAATGGCAACCCTGGGCCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGCTC GAGGAGACACTGGTGCCCCTGGCAGAGCTGGTGACCCTGGACTTCAAGGACCCGCAGGAGCTCCTGGAGA GAAAGGCGAACCTGGAGATGACGGTCCCTCTGGTTCTGATGGTCCCCCAGGTCCCCAGGGGCTGGCTGGT CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCAGGCCTTCCCGGCCCATCGG GTGAGCCCGGCAAGCAGGGTGCACCTGGTGCATCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC TGGCTTGACAGGACCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGCAGA GATGGTGCGGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCACTTGGTGCTCCTGGAGCTCCTGGGC CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG TCCTATGGGCCCCTCAGGACCTGCTGGAGCCCGTGGAATTGCTGGCCCTCAAGGCCCCCGAGGTGACAAA GGAGAAGCTGGAGAGCCTGGCGAGAGAGGACTGAAGGGGCACCGAGGTTTCACTGGACTGCAGGGTCTGC CTGGCCCCCCGGGTCCTTCTGGAGATCAGGGTACTTCTGGCCCTGCTGGTCCTTCCGGCCCTAGAGGTCC ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT CCCCGTGGACGCTCAGGAGAAACTGGCCCTGCTGGTCCTCCTGGAAATCCTGGTCCCCCTGGCCCTCCGG GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGACAGAGAGAGAAGGGCCCCGATCC CCTGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCATGACGTCGAGGTGGACGCCACG CTCAAGTCGCTGAACAACCAGATCGAGAGCATCCGCAGCCCTGATGGCTCCCGCAAGAATCCCGCTCGCA CCTGCCAGGACCTGAAACTCTGCCACCCAGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG CTGCACCTTGGACGCCATGAAAGTCTTCTGCAACATGGAGACTGGCGAGTCTTGCGTCTACCCCAACCCA GCGACTGTGCCTCGGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAGCACATCTGGTTTGGAGAGA CCATGAACGGCGGCTTCCACTTCAGCTACGGCGACGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATTGCC TACCTGGACGAAGCAGCCGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTGGAGATGA GGGCCGAGGGCAACAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACACACCGGTAAGTG GGGCAAGACCATCATCGAGTACCGATCACAGAAGACCTCACGCCTTCCCATTGTTGACATTGCACCCATG GACATCGGAGGGCCTGATCAGGAATTTGGTGTGGACATAGGGCCTGTCTGTTTCTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012929
Insert Size	4260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012929.1, NP_037061.1
RefSeq Size	4538 bp
RefSeq ORF	4260 bp
Locus ID	25412
UniProt ID	P05539
Gene Summary	structural component of the cartilage extracellular matrix [RGD, Feb 2006]

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