Nek1 (NM_001106082) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214766 representing NM_001106082 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCTGTTCTTGTTAAATCTA CAGAGGATGGCAGACATTATGTCATCAAGGAAATTAACATCTCAAGAATGTCCGATAAAGAAAGAGAAGA ATCAAGGAGAGAGGTCGCAGTGTTGGCAAACATGAAGCATCCAAATATTGTCCAATACAAAGAATCATTC GAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAAGGAGGTGATCTGTTTAAACGAATAAATG CTCAGAAAGGCACTTTGTTTCAAGAAGATCAGATTTTGGACTGGTTTGTTCAGATATGTTTGGCTTTGAA GCATGTACACGATAGAAAAATTCTTCACCGAGACATAAAGTCACAGAACATATTTCTAACCAAAGATGGG ACAGTGCAGCTTGGAGATTTTGGAATTGCTCGAGTTCTCAATAGTACTGTAGAGCTGGCTCGAACTTGCA TAGGGACTCCATACTACTTGTCACCTGAAATCTGTGAAAACAAGCCTTACAACAATAAAAGTGACATTTG GGCTTTGGGCTGTGTCCTTTATGAGTTGTGTACACTTAAACATGCATTTGAAGCTGGAAACATGAAAAAC CTGGTACTGAAGATAATATCTGGATCCTTTCCTCCAGTGTCTCTACATTATTCCTATGATCTCCGCAGCT TGCTCTCTCAGCTGTTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGCTT TATAGCTAAACGAATCGAAAAGTTTCTCTCACCTCAGCTTATTGCAGAAGAATTTTGTCTGAAAACAGTT TCAAAGTTTGGACCACAGCCTATCCCAGGTAAAAGACCAGCATCAGGACAAGGTGTCAGTTCTTTTGTCC CTGCTCAGAAAATCACAAAGCCTTCTGCTAAATATGGAGTGCCTATAACATATAAGAAGTACGGAGATAA AAAGTTACTTGAAAAAAAACCACCCCCAAAGCATAAGCAGGCCCATCAGATTCCAGTGAAGAAAATGAAT TCTGGAGAAGAAAGGAAGAAAATGTCTGAGGAAGCAGCAAAAAAACGAAGGTTGGAATTTATTGAGAAAG AAAAGAAGCAAAAGGATCAGATCAGGTTCCTGAAGGCTGAGCAGCTGAAGAGGCAAGAGAAGCAGCGGTT GGAGAGGATAAATAGGGCCAGGGAACAAGGATGGAGGAATGTTTTAAGGGCTGGTGGAAGCGGTGAAGTA AAGGCTTCCTTTTTTGGCAGTGGAGGAGCTGTGTCTCCATCACCTTGTTCTCCTCGGGGCCAGTATGAAC ATTACCATGCCATCTTTGACCAAATGCAGCAGCTAAGAGCAGAAGATAATGAAGCGAGATGGAAAGGGAG AATATATGGTCGATGGCTCCCTGAAAGGCAAAAAGGACACCTAGGTGTAGAGAGAGCCAAACAAGTGGAA GAATTCCTACAGCGTAAACAAGAAGCTATGCAGAATAAAGCCCGAGCAGAAGGACATGTGGTTTATCTGG CAAGACTGAGGCAAATAAGACTACAAAATTTTAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGA GAATAAAGAAGCTGGTGGTACCAAAGGACAAGACGCAACTGAAGAGGCTGACATGAGGCTCAAAAAGATG GAGTCACTTAAGGCCCAAGCAAATGCACGTGCTGCTGTACTAAAAGAACAGCTAGAGCGAAAAAGAAAGG AAGCTTATGAAAGAGAAAAGAAAGTGTGGGAAGAACATTTGGTGGTGAGGGTAAAAAGCTCAGATGTCCC TCCATCTATGGAGCATCATGAAGCAGGTGGTTCTCCATCACAGCAGCAGATGAAACCTGTTATTTCTGTG ACTTCAGCTTTGAAAGAAGTGGGCCTGGATGGAAGTTTAACTGAGTCCCAAGAAACTTCAGAAGAAATGG AAAAGAGTAACAATGCTATTTCAAGTAAGCGAGAAATACTGCGTAGGCTGAATGAAAATCTTAAAGCTCA AGAGGATGAAAAGGAAAAGCATCATCACGCTGATTCTTGTGAGACCGTTGGTCACAGAGATGAGAGAGAG TATGAGAAGGAAAATGCCATTTCCTCTGATCGCAAGAAATGGGAGATGGGAGGTCAGCTTGTGATTCCTC TGGATGAAGTGACACTGGATGCATCCTTCTCTGCTTCTGAAAAACATACTGTGGGAGAGGTTATTAAGTT AGATTCTAGTGGCTCTCCAAGAAAAGTCTGGGGGAAAAGCCCTACAGATTCTGTGCTGAAGATACTTGGA GAAGCTGAATTACAGCTACAGACAGAACTACTAGAAAACACGCCTTTTAAAAGTGAGGTTTGTGTTGAAG AGGAGAACTACAAACCCCTACTCACTGGAGAAGAGAATCTGCAGTGTATTTCAAAAGAAGTAAACCCATC AGCCACTGTTGATACTGAAACAAACAGTCCAAAGTTTGCTAAGGTGTCTCCAGCAATGTCCGAAGGAAAT GTGGAAGAACCTGATGATTTGGAAACAGAAGTTCTACAAGAGCCAAGTGCCATACCCTCAGATGGGAGTT TGCCGCCTGTTGTTAATGAGGTGTGGACCAGAGCTAAGGCAACTGGGTTGGAGGATAGCATTGCTGTGCA GCAGAATGACGTTTGTGAAGAGCGAATTCCAGGGACTGTGGACCAGTCCTGTAAGGATCATATAGAACCT GCAGGAAACGATTCTCCGCAGTCTGGCTGTGATGAAGAGAAGTCAGTACAGCCAGAATTGTTTTTCCAGA AAGTGGTTCACTCCAAGGACTTGAACTTAGTTCAGTCAGCTCATCGTTCCCCAGAAGAAGCAATTCCAAT TCGATCTCACTCTGATTCTCCACCAAAAGCTAAAAGCAAGAATTCCCTACTGATTGGACTTTCCACTGGT CTGTTTGATGCAAACAACCCAAAGATGCTGAGGACCTGTTCACTTCCAGACCTTTCCAAGCTCTTCAGAA CCCTAATGGATGTTCCCACTGTGGGAGACGTTCATCAAGACAGTCTTGAAATAGAGGAGCTTGAGGATGA ACATATTAAAGAAGGGCCCTCGGATTGCGAGGACATTGTATTTGAAGAAACTGACACAGATTTACAGGAG CTTCAGGCCTCAATGGAGCAGCTGCTTAGGGAGCAACCCGGTGACGAATACAGTGAGGAGGAAGAGTCTG TCTTAAAAAGCAGCAATGTGGAGCGGGCAGCAAGAGGGACAGATGTCCCAGATGAGGAGGACAACCCCAG CAGTGAAAGCGCCCTAAACGAGGAATGGCACTCAGATAACAGTGATGCTGAGACTACTAGTGAGTGTGAA TATGACAGTGTCTTTAACCATTTAGAGGAACTAAGACTTCACTTGGAACAAGAAATGGGCTTTGAAAAGT TCTTTGAGGTCTATGAGAAAGTAAAGGCTATTCATGAGGATGAAGATGAAAATATTGAAATTTCTTCAAC CATAGTCGAGAACATCTTGGGCACTGAGCATCAGCACCTTTATGCCAAGATTCTGCATCTAGTCATGGCA GACGGAGCCTATCAGGAAGATAATGATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106082
Insert Size	3603 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106082.2, NP_001099552.2
RefSeq Size	4895 bp
RefSeq ORF	3603 bp
Locus ID	290705

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