Golga3 (NM_001107847) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214857 representing NM_001107847 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGAGCATCAGCCAAACAGGATGGCCTCTGGGAAAACAAGTCTAGCAGTGATATTTCATCTTGCC CTGAAGCCTCACTGGAAACTGTGGGCTCTTTGGCCCAACTGCCTGACCAACAAGATACAGCTCAGGATGG CAGTGTTGAGGTAAACAGAGTTTTTAAGGAAGAAGGAAGCCCAGACAGATCCAGCCAGGTGCCCATCTGT CAGAATGGGCAGATCCCAGACCTTCAGTTGTCTCTTGATCCCACAACAAGCCCAGTGGGACCTGATGCCT CTACAGGCTCTACTGCATCTTCACTGCCCTTGGAAAAGGAGGAGCAGGTCCGACTGCAGGCCCGGAAGCG CCTGGAAGAGCAGCTGATGCAATACCGAGTTAAGCGCCATCGGGAAAGGTCCAGTCAGCCGGCAACTAAA ATGAAACTTTTTAGTACACTTGATCCTGAGCTCATGTTAAATCCAGAAAACTTGCCAAGGGCCAGTGCTG TAGCTGTGACAAAAGAATATTCCTTCCTGCGTACCAGTGTTCCTCGGGGGCCCAAGGTAGGCAGCTTAGG GCTGCTGGCACATTCTAAAGAGAAGAAGAATTCCAAGTCAAGCAAGATTCGGTCTCTGGCTGACTATAGA ACTGAAGATCCAAGTGACTCTGGTGGCCTTGGTTCTACTGCTGATGCTGTTGGAAGTTCCCTGAAACAGA GTAGAAGTAGCACTTCAGTTGTGTCTGAAGTGAGCCCATCATGTGAGACTGATAACCGTGCAGAGAGTGC CTCACTGGCTGGGGACAGTGTGTCAGAGGCTGATGGAAATGAGAGTGACAGTTCCTCACACAGCAGCTTC TCTGCCCGAGGGGCCTGTGCTGTCCTGGGGAATGTCGGCACACCAGGAGCAGCTTACGTGGTTGATGGCC AGGAGATTTCTGCTGAGGCCCTGGGCCAGTTTCCTTCGATTAAAGACGTGCTACAGGCTGCAGCAGCCCA GCACCAAGATCAGAACCAGGAAGCCAATGGGGAGGTGCAGAGCCGCAGAGACAGCATCTGCAGCAGTGTG TCCATGGAGAGCTCTTTTGCTGAACCACAGGAAGAATTGTTGCAGATCCTTAAAGATAAGAGGAGACTTG AAGGACAGGTGGAGGCTTTGTCATTAGAAGCCAGTCAGGCACTTCAAGAGAAGGCTGAGCTACAGGCCCA GCTTGCTGCCCTGAGCACAAGGCTACAGGCACAGGTGGAACACAGTCACAGCAGCCAGCAGAAGCAAGAC TCCCTTAGTTCTGAAGTGGACACTTTGAAGCAGTCTTGCTGGGATTTGGAGCGGGCTATGACTGACCTGC AGAACATGCTAGAAGCTAAAAATGCCAGCCTGGCATCGTCAAACAATGACCTACAAGTGGCAGAGGAGCA GTATCAGAGACTGATGGCCAAAGTGGAGGCCATGCAGAGAAACATCCTTAGCAAGGACAACACAGTTCAT GACCTGCGGCAGCAGATGACAGCGCTACAGAGCCAGCTCCAGCAAGTACAGCTGGAGCGCACAACACTGA CCAGCAAACTACAAGCATCACAGGCTGAAATCACTTCTTTGCAGCAGGCCCGGCAGTGGTACCAACAGCA GCTTGCTTTGGCCCAGGAGGCCAGAGTCAGGCTGCAAGGAGAGATGGCCCACATCCAGGTCGGACAGATG ACTCAAGCGGGCCTTTTGGAGCACCTGAAACTTGAGAATGTGTCCCTATCGCATCAGCTAACAGAAACTC AGCACAGATCCATCAAGGAAAAAGAGCGCATAGCTGTTCAGCTCCAGAGCATTGAGGCTGACATGTTAGA TCAGGAGGCAGCATTTGTACAGATTCGGGAGGCGAAGACAATGGTGGAAGAAGACCTCCAAAGGAAGCTG GAAGAATTTGAAGGTGAACGGGAACAGTTACAGAAAGTGGCGGATGCAGCAGCGTCCCTGGAGCAACAGT TGGAACAGGTGAAGTTGACATTGTTCCAGCGAGATCAGCAGCTGGCAGCACTTCAGCAGGAGCATCTAGA TGTGATAAAGCAGCTTACATCGACACAGGAGGCTCTACAGGCCAAAGGGCAGTCCCTCGATGACCTGCAA ACACGTTATGATGAGCTACAAGCAAGGCTGGAAGAGCTGCAGAGAGAGGCTGACTCCAGGGAGGATGCCA TTCACTTCTTGCAGAATGAGAAGATTGTCTTAGAAGTGGCTCTGCACTCAGCCAGGAGTGACAAGGAGGA ACTTGACAGAGGAGCACGGCGTTTGGAAGAAGATACTGAGGAGACATCAGGACTTTTAGAACAATTGAGA CAAGATGTGGCTGTCAAGTCCAACCAGGTGGAACACTTGCAGCAAGAGACAGCCACATTGAGAAAACAGA TGCAGAAAGTAAAGGAGCAGTTTCTTCAGCAAAAGGTGATGGTAGAGGCCTATCGGCGAGATGCTGCTTC TAAAGACCAACTCATCAATGAGCTGAAAGCTACCAAAAAACGCCTGGACTCAGAGATGAAGGAACTGAGG CAGGAGCTGATCAAGCTGCATGGAGAGAAGAAGACTGTGGAAGTAGAGCACTCACGGCTGCAGAAGGACA TGTCCCTGGTTCACCAGCAGATGGCAGAGCTTGAGGGGCATCTCCAGTCGGTGCAGAAAGAGCGAGATGA AATGGAGATTCACCTGCAGTCTTTGAAGTTTGACAAAGAGCAGATGATAGCCCTTACTGAGGCCAATGAG ACACTAAAGAAACAAATAGAGGAGCTGCAGCAAGAGGCCAAGAAGGCCATCACAGAACAGAAGCAGAAGA TGAAGCGGCTGGGCTCAGATCTGACCAGTGCCCAGAAGGAGATGAAGACTAAGCACAAGGCTTACGAGAA TGCTGTGAGCATTCTCAGCCGTCGCCTACAGGAGGCTCTGGCTAACAAGGAGGCCACAGATGCGGAGCTG AACCAGCTCAGAGCTCAGAGCACAGGTGGCAGCAGTGACCCTGCTCTACATGAGAAGATTCATGCTCTGG AGGTGGAACTGCAGAATGTTGGCCAAAGCAAAATACTGCTGGAGAAGGAGTTACAGGAGGTGATCACGAT GACTAGCCAGGAGTTGGAAGAGTCCCGGGAGAAGGTGCTGGAGCTCGAGGATGAGCTTCAAGAATCCAGA GGCTTCAGAAGGAAGATAAAACGTCTTGAAGAGTCAAATAAGAAGCTAGCTCTGGAGTTAGAACATGAGC GAGGGAAGCTCACCGGCCTTGGACAATCTAATGCGGCTTTGCGGGAGCACAACAGCATTCTGGAAACTGC ACTAGCCAAGAGGGAAGCTGACCTGGTGCAGCTGAATCTCCAGGTGCAGGCAGTTTTACAGCGCAAAGAG GAGGAGGATCGCCAGATGAAGCAACTTGTCCAAGCCTTACAAATCTCATTAGAGAAAGAAAAGACGGAAG TGAGCAGCCTGAAGGAACAGATGGCTGCTGCCAGGGTAGAAGCTGGGCACAACCGCCGCCACTTTAAGGC AGCGACTTTGGAGCTGAGTGAGGTGAAGAAAGAATTGCAGGCCAAAGAACACCTGGTACAGACACTGCAG GCCGAGGTGGATGAGCTCCAGATTCAGGAGGGAAAGCATTCTCAAGAAATCGCTCAATTCCAGACTGAGC TGGCAGAAGCTAGGACCCAGCTCCAGCTCCTGCAGAAGAAGCTCGAAGAACAGAGGAGCCAGCAACCAAC AGGGAGCCAAGAGATGGAAGATCTCAAATGGGAATTGGATCAGAAAGAACGAGAGATTCAGTCCCTGAAG CAGCAGCTTGACTTGACAGAGCACCAGGGCAAGAAAGAACTCGAGGGTACACAACAGACACTGCAGACTA TCAAGTCTGAGTTGGAAACGGTTCAGGAAGATCTGTCTGAGACCCAGAAGGATAAGTTTATGCTTCAAGC CAAAGTGTCTGAACTGAAGAACAACATGAAGACTCTGCTGCAGCAGAACCAACAGCTCAAGCTGGATCTC CGCCGCGGTGCAGCCAAGAAGAAAGAGCCAAAAGGTGAGTCCAGCTCATCCAGTCCAGCAACACCCATCA AGATACCAGACTGCCCTGTCCCAGCCTCACTGCTGGAGGAGCTGCTGAGGCCCCCACCCGCTGTGAGCAA GGAGCCCCTGAAGAATCTCAACAGCTGTTTGCAGCAGCTCAAGCAGGAAATGGACAGTCTACAGCGCCAG ATGGAGGAGCATACCATCACTGTGCATGAGTCCCTGTCCTCGTGGGCTCAGGTGGAAGCTGCCCCAGCAG AGCATGCCCATGCCCGGGGAGACACAAAAGTACACAATCAAAACAGTGTTCCTGGAGAAGGGCTGGGCCA ATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107847
Insert Size	4344 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107847.1, NP_001101317.1
RefSeq Size	5719 bp
RefSeq ORF	4344 bp
Locus ID	312077

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