Col1a2 (NM_053356) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214908 representing NM_053356 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGCTTTGTGGATACGCGGACTCTGTTGCTGCTTGCAGTAACGTCGTGCCTAGCAACATGCCAAT CTTTACAAATGGGATCTGTACGGAAGGGCCCCACTGGAGACAGAGGACCGCGTGGACAAAGGGGCCCAGC AGGTCCCCGAGGCAGAGATGGTGTTGATGGTCCCGTTGGCCCTCCTGGTCCCCCTGGTGCCCCTGGCCCC CCTGGTCCCCCTGGCCCCCCTGGTCTTACTGGGAACTTTGCTGCTCAGTATTCTGACAAAGGAGTTTCAG CTGGCCCCGGACCAATGGGCTTAATGGGACCCAGAGGCCCTCCTGGTGCTGTCGGAGCCCCTGGCCCTCA AGGTTTCCAAGGACCTGCTGGTGAGCCTGGTGAGCCCGGACAAACGGGTCCTGCAGGTTCCCGTGGGCCA GCTGGCCCTCCTGGCAAGGCCGGTGAAGATGGTCACCCTGGAAAACCTGGAAGACCTGGCGAGAGAGGAG TCGTTGGGCCACAAGGTGCTCGTGGTTTCCCTGGGACTCCTGGACTTCCTGGCTTCAAAGGCATTCGAGG ACACAACGGTCTGGATGGATTGAAAGGACAGCCTGGTGCCCAGGGTGTGAAGGGTGAACCCGGTGCCCCT GGAGAGAATGGAACCCCAGGTCAAGCAGGAGCCCGAGGGCTTCCTGGTGAGAGAGGGCGTGTCGGAGCTC CTGGTCCAGCTGGTGCCCGTGGCAGTGATGGCAGTGTTGGCCCCGTCGGCCCTGCTGGTCCCATTGGGTC TGCTGGTCCTCCTGGTTTCCCAGGTGCTCCTGGTCCCAAGGGTGAACTTGGGCCTGTTGGTAACCCTGGC CCTGCTGGTCCTGCTGGTCCCCGTGGTGAAGCGGGTCTTCCGGGTCTTTCTGGCCCCGTTGGACCTCCTG GCAACCCTGGAGCAAATGGCCTCACTGGTGCCAAGGGAGCCACTGGACTCCCTGGTGTTGCTGGGGCTCC TGGTCTCCCCGGTCCCCGTGGTATTCCTGGCCCCGTTGGTGCTGCTGGTGCCACTGGTCCCAGAGGACTT GTTGGTGAACCTGGTCCTGCTGGCTCCAAAGGAGAGACTGGTAACAAGGGTGAGCCTGGCTCTGCTGGAG CCCAAGGTCCTCCTGGTCCCAGCGGTGAAGAAGGAAAGAGAGGGTCCCCTGGAGAGCCTGGATCTGCTGG TCCTGCAGGGCCTCCAGGGCTGAGAGGCAGCCCTGGTTCTCGAGGTCTTCCTGGAGCTGATGGCAGAGCT GGTGTGATGGGCCCTCCTGGCAATCGTGGTTCAACTGGCCCTGCTGGAGTCCGAGGTCCTAATGGAGATG CCGGTCGCCCTGGAGAACCTGGTCTCATGGGACCCAGAGGTCTTCCCGGGTCTCCTGGAAATGTTGGCCC AGCTGGTAAAGAAGGCCCTGTGGGTCTCCCTGGCATCGATGGCAGACCTGGCCCAATTGGCCCAGCTGGA CCAAGAGGTGAAGCTGGCAACATCGGATTCCCTGGACCCAAAGGCCCCTCTGGTGATCCTGGCAAACCTG GTGAGAAAGGCCATCCCGGTCTCGCTGGTGCTCGGGGAGCTCCAGGTCCCGACGGCAACAATGGTGCTCA GGGCCCTCCTGGACCTCAGGGTGTTCAAGGTGGCAAAGGTGAACAGGGCCCTGCTGGTCCTCCAGGCTTC CAGGGTCTCCCGGGTCCCTCTGGTACCGCTGGAGAAGTTGGCAAGCCGGGAGAAAGGGGTCTTCCCGGTG AATTCGGTCTCCCTGGTCCTGCTGGTCCAAGAGGAGAACGTGGTCCCCCAGGTGAGAGTGGAGCTGCTGG TCCTTCTGGTCCTATTGGAATCCGAGGTCCCTCTGGAGCCCCAGGGCCTGATGGGAACAAGGGTGAAGCT GGTGCAGTCGGTGCTCCAGGCTCTGCTGGTGCCTCTGGTCCTGGTGGGCTTCCAGGAGAGAGGGGTGCTG CTGGCATACCTGGAGGCAAAGGAGAAAAGGGTGAAACTGGTCTCAGAGGTGAAATCGGCAACCCTGGTAG AGATGGTGCTCGAGGTGCTCCTGGTGCTATAGGTGCCCCTGGTCCCGCTGGAGCCTCAGGTGACCGGGGC GAAGCTGGTGCTGCTGGTCCTTCTGGCCCTGCTGGTCCTCGAGGTAGCCCTGGTGAACGTGGTGAGGTTG GTCCTGCTGGCCCCAATGGATTTGCTGGTCCTGCTGGTTCTGCTGGCCAACCTGGTGCTAAAGGAGAAAA GGGAACCAAAGGGCCTAAGGGAGAAAATGGCATCGTTGGTCCAACCGGACCTGTCGGAGCTGCTGGCCCA TCTGGTCCTAATGGCCCACCTGGCCCTGCTGGAAGTCGTGGTGATGGAGGGCCCCCTGGTATGACTGGCT TCCCTGGTGCTGCTGGACGGACTGGTCCCCCCGGACCCAGTGGTATTACTGGCCCCCCTGGTCCCCCTGG TGCTGCTGGTAAAGAAGGTATTCGTGGTCCCCGTGGTGACCAAGGTCCAGTTGGCCGAACTGGAGAAATA GGTGCATCTGGTCCCCCTGGATTTGCTGGCGAGAAGGGTCCTTCTGGAGAACCTGGTACCACAGGACCCC CTGGCACTGCAGGTCCTCAGGGTCTTCTTGGTGCTCCCGGTATTCTGGGTCTCCCTGGCTCTCGAGGTGA ACGTGGCCAGCCAGGTATTGCTGGTGCTCTGGGTGAACCTGGTCCTCTGGGCATTGCAGGCCCTCCTGGG GCCCGTGGTCCCCCTGGTGCTGTGGGCAGTCCTGGAGTTAATGGTGCCCCTGGTGAAGCCGGTCGTGATG GTAACCCTGGCAGTGATGGTCCCCCAGGTCGTGATGGTCAACCCGGACACAAGGGAGAGCGTGGTTACCC TGGCAACATTGGTCCCACTGGTGCTGCAGGTGCTCCTGGTCCTCATGGTTCTGTGGGTCCTGCTGGGAAA CATGGAAACCGTGGTGAGCCTGGTCCTGCTGGTTCTGTTGGTCCCGTTGGTGCTGTCGGTCCAAGAGGTC CTAGTGGCCCTCAAGGTATTCGAGGTGACAAGGGAGAGCCTGGTGACAAAGGGGCCAGAGGTCTTCCTGG CTTAAAAGGACACAATGGATTACAGGGTCTTCCTGGTCTTGCTGGGCTGCATGGTGACCAAGGAGCTCCT GGTCCCGTGGGTCCTGCTGGTCCCAGGGGTCCTGCTGGTCCTTCTGGCCCCATTGGCAAAGATGGTAGAT CTGGACATCCTGGACCAGTCGGTCCTGCTGGCGTTCGTGGCTCTCAGGGTAGCCAAGGTCCCGCTGGTCC CCCTGGTCCCCCTGGGCCTCCTGGGCCTCCTGGTGTCAGCGGAGGAGGCTATGACTTTGGTTTCGAAGGA GGCTTCTACAGGGCTGACCAGCCTCGCTCACAGCCTTCACTCAGACCCAAGGACTATGAAGTTGATGCAA CTCTGAAATCTCTCAATAACCAAATCGAGACCCTTCTCACTCCTGAAGGCTCTAGAAAGAACCCTGCCCG CACATGCCGTGACTTAAGACTCAGCCACCCAGAGTGGAAGAGCGATTACTACTGGATTGACCCTAACCAA GGATGCACTATGGATGCCATCAAAGTGTACTGCGATTTCTCTACTGGTGAAACCTGCATCCAGGCCCAAC CTGTCAACACCCCAGCCAAGAATGCATACAGCCGTGCCCAGGCCAACAAGCATGTCTGGTTAGGAGAGAC CATCAATGGTGGCAGCCAGTTTGAATACAACGCAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTC GCCTTCATGCGCCTGCTAGCCAACCGTGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGT ACCTGGACGAGGAGACAGGCCGCCTGAATAAGGCTGTCATTCTGCAGGGCTCCAACGACGTCGAACTTGT TGCTGAGGGCAACAGCAGATTCACCTACACTGTCCTTGTCGATGGCTGCTCCAAAAAGACAAATGAATGG GACAAGACAGTCATTGAATACAAAACGAATAAGCCATCTCGCCTGCCATTCCTTGACATTGCACCTCTGG ACATCGGTGGTACTAACCAAGAATTCCGTGTGGAGGTTGGCCCTGTCTGTTTCAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053356
Insert Size	4119 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053356.1, NP_445808.1
RefSeq Size	4474 bp
RefSeq ORF	4119 bp
Locus ID	84352
UniProt ID	P02466
Gene Summary	structural constituent of the extracellular matrix [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.