Disp1 (NM_001105983) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214939 representing NM_001105983 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCTGTGATCAGCGGAAGTGATTCTGTGCTTCTGAGCAATGGCAGCATCTCCACCAGCACTACCAACC CTAGTCCCCTCACCCCCAGTGATGGAGACCTCACAGCTCAGCAGCTCGCACCCAGAGAAACCCCGAGGAC AAAAGCAAGTCCAAATGGATGCCTGCAACTTAATGGCACAGTTAAGTCATCCTTTCTGCCTTTAGACAAC CAAAGAACGCCTCAGACAGCTCTGTGCTGCCACCCTTGTCCATACCATCACCCTGTGACCAGCCATACCA GTCACCAAGAGTGCCATCCTGAGGCTGGCCTTGCAGCATCCTCCGCTTTGACCTCGGGTTGCATACAGCC ACATTCCGAATACTCTGCCTCTCTGTGTCCAAACCATTCACCTGTGTATCAGGCTGCGCACTGCCGTCAG CCCTCGCCATCTCTCTGCCTTCATCACCCGTGGCCTGACCATTTCCAGCACCAGCCTGTACGGCAGCACC TGGCCATCATCGGGCCGTCCAGACCTTTCAGGTTCCCGAGAAGTTATGCTGCCCTGCTAGCCGACTGGCC TGTGGTGGTCCTGGGCTTGTGCACGCTGCTCATCGTGGTCTGCGCCCTGGTGGGAGTCTTAGTGCCGGAG CTCCCCGACTTCTCTGACCCACTGCTGGGTTTTGAACCAAGAGGGACTGCAATAGGACAGAGGCTGGTCA CCTGGAATAACATGATGAAAAACACAGGCTACAAAGCGACGTTGGCCAACTATCCGTATAAGTATGCAGA AGAGCAAGCCAAAAGCCGCCGGGATGACAGATGGTCAGGCGATCATCATGAGAGAGAGAGAAGAGAAGTA GACTGGAACTTCCAGAAGGACAGCTTCTTCTGCGACGTTCCAAGTGATGGCTACTCCAGAGTGGTGTTTG CTTCAGCAGGAGGGGAGACCCTGTGGAATTTACCTGCAATTAAGTCAATGTGTGATGTGGATAACTCCAG GATCAGATCCCACCCCCAGTTCAGCGACCTCTGCCAGAGGACCACCGCCGTCTCCTGCTGCCCCAGCTGG ACCCTGGGGAATTACATCGCCATTCTGAATAACAGATCGTCCTGTCAGAAGATTGTCGAGCGAGATGTCT CTCACACGCTGAAGCTGCTTCGGACCTGTGCCAAGCACTACCAGAACGGCACCCTGGGGCCAGACTGCTG GGACAAGGCGGCCAGAAGGAAGGACCAGCTCAAGTGCACCAACGTGCCACGCAAGTGTACCAAATACAAC GCCGTGTACCAGATTCTCCACTACTTGGTAGACAAGGACTTCATGACCTCCAAGACGGCTGACTACGCCG TGCCAGCTTTAAAGTACAGCATGCTCTTCTCCCCCACGGAGAAAGGGGAGAGCATGATGAACATCTACCT GGACAACTTTGAGAACTGGAACTCTTCGGACGGCATCACCACCGTGACAGGGATCGAGTTTGGCATCAAG CACAGTCTGTTTCAGGATTACCTCCTCATGGATACCGTGTACCCCGCCATCGCCATCGCCATCGTCCTTC TCATCATGTGTGTCTACACCAAGTCCGTGTTCATCACGCTCATGACCATGTTTGCCATCATCAGCTCCCT GATCGTCTCCTACTTCCTTTACCGAGTCGTGTTTAACTTCGAGTTCTTCCCATTCATGAACCTCACTGCA CTCATCATCCTGGTCGGGATCGGCGCCGACGACGCCTTCGTCCTGTGCGACGTCTGGAACTACACCAAGT CCGACAAGCCCCGTGCAGAAACGTCTGAGGCGGTAAGCGTCACCTTGCAGCATGCAGCCCTCTCCATGTT TGTCACCAGCTTCACCACCGCCGCCGCCTTCTACGCTAACTACGTCAGTAACATCACGGCAATCAGGTGC TTCGGGGTGTATGCGGGGACCGCTATATTGGTGAACTATGTCCTGATGGTTACCTGGCTTCCGGCTGTCA TTGTTCTGCATGAGCGCTATCTCCTCAATATATTCACCTGCTTCAGAAAGCCCCAGCCGCAGGCCTACCA CAAGAGCTGCTGGACAGTGCTCTGCCGGAAGGGCCGCAAGGCGCTTTTCGCCATCTCAGAGGCATCTCGG ATTTTCTTTGAAAAGGTGCTGCCCTGCATCGTCATCAAGTTCCGCTACCTTTGGCTCGTCTGGTTCCTTG CCCTGACTGTGGGCGGGGCCTACATCGTGTGCATAAACCCAAAGATGAAACTGCCTTCCTTGGAGTTATC TGAGTTCCAGGTGTTCAGGTCCTCTCATCCTTTCGAGCGCTATGATGCGGAGTTCAAGAAGCTGTTCATG TTCGAGCGGGTTCACCATGGAGAGGAGCTCCACATGCCCATCACAGTAATCTGGGGCGTGTCTCCAGAAG ACAGCGGTGACCCTCTGAACCCCAAGAGCAAAGGGGAGCTGACACTCGATAGCACGTTCAACATTGCTAG CCCGGCCTCCCAGGCCTGGATTTTACACTTCTGTCAGAAACTGAGGAACCAGACCTTCTTCCACCAGACT GAGCAGCAGGACTTCACCAGCTGTTTCATCGAGACCTTCAAACAGTGGATGGAGAACCAGGACTGTGACG AGCCGGCCCTGTACCCCTGCTGCAGCCACTGCAGCTTCCCCTATAAGCAAGAGGTTTTCGAGCTGTGCAT CAAGAAGGCCATCATGGAGCTGGACAGGAGCACGGGGTACCACCTGAACAACAAGACCCCGGGGCCTAGG TTCGACATCAACGACACCATCAGGGCCGTGGTGCTGGAGTTCCAGAGCACCTTCCTCTTCACCTTGGCGT ATGAGAAGATGCAGCAGTTCTACAAGGAGGTGGACTCATGGATTTCCCACGAGCTGAGTTCGGCCCCCGA GGGCCTCAGCCACGGCTGGTTCGTCAGCAACCTGGAGTTCTATGACCTGCAGGACAGCCTTTCCGATGGC ACCCTCATTGCCATGGGGCTCTCGGTGGCCGTCGCGTTTAGTGTGATGCTGCTGACCACATGTAATATCA TCATAAGCCTGTATGCCATCGCCTCCATAGCTGGGACCATATTTGTCACAGTTGGGTCCCTTGTCCTGCT GGGCTGGGAGCTGAACGTGCTGGAGTCCGTCACCATCTCGGTTGCAGTTGGCTTGTCGGTGGACTTTGCA GTCCACTACGGGGTGGCATACCGCCTGGCCCCAGATCCCGACCGAGAGGGCAAGGTCATCTTCTCTCTGG GTCGTATGGGCTCTGCGATCGCCGTGGCTGCACTGACCACCTTCGTGGCGGGTGCCATGATGATGCCCTC CACGGTCCTGGCTTACACGCAGCTGGGCACCTTCATGATGCTTGTCATGTGCGTCAGCTGGGCCTTCGCC ACCTTCTTCTTCCAGTGCCTGTGTCGGTGCCTGGGACCTCAAGGCACCTGTGGCCAGATCCCTTTACCCA AAAAGCTCCAGTGTAGTGCCTTTTCCCACACCTCGGCTGCGAGGCCTGGGGGCAGGGCACCAGGCAAAGC CACCAACGCTTACAGTGTAGATGCCAGGGGCCAGAAATCCGAACTGGAACATGAGTTGTATGAGTTACAG CCCCTGGGCTCTCACAGCTGCACTTCCTCAGACATGACCGCTTATGAAGGGCCATGCATCTGCTCCGAAT TTTTCAATGGCCAAGCAAAGAATTTAAGGATGCCCATGTCTGCAGCCTACAGCAGCGAACTCACCAAAAG CCCCAGAAGTGAGCCAGGCTCGGCCTTGTTGCAGCCCTGTCTGGAGCAGGACACCATGTGTCACTTCTCT CTCAATCCGAGATGTAACTGCCCCAATGCCTACACCCACCGAAAGTATGGATTGCCAGAAATCCACTGCC AGCAGATGGGTGACTCCTTATGCCACAAGTGTGCCTCCACCGCAGGCAGCTTTGTCCAGATTCAGAATGC TGCGGCGCCTCTAAAGGCCACCCACCACGCCGCCGAAGGCCTTCTGCATCCTGTCCAGCACATGCTGCCA CCAGGGATGCAGAATTCTCAGTCTAGGAATTTTTTTCTCCACCCAGTCCAGCACTTTCAGGCCCAAGAGA GCCTGGGTCCCACCAGCACCCGGGGTCGTGAGAACACAGATGAGCGTCCGCCCCGGACAGCAGAGCCACC ACCAGCAGGAGCCAGCAGGAGCTCTGAGTCTTTCCAAAGAGCTTGCTGCAATCCTGAGAATAACCAAAGG AGACTCTGCAAAAGCAGAGACTCGGGGGACACAGAGGGCAGTGGAGGGACAGAAACCAAGGTTTGTGGTT TAGCAAACCAGACTGACAAGGTAGAGAAGGAAGTGAAGCCCAGCCTGCTGCAGACAGATGAAATCGTGAA CTCAGAACAGTTAAATCATGATGAACCAAAACTTATATTCAGCCATTTAATACGGGAGGCCGGCTGCAGG CCCTGCCCAGACAGGCCGCAGGGCTGTAGAAGCCTGAGACTGAAGTGCGGGTCCACGGACTGCCAGGCGC CAGACCTTGAAGCCAATGTGCCTGCTGTACCAACCCACTCAGACCTGTCTGTGTCTGGCGAAAGCCTGTT AATAAAAACACTACAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_001105983
Insert Size	4569 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105983.1, NP_001099453.1
RefSeq Size	5031 bp
RefSeq ORF	4569 bp
Locus ID	289338

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.