Hcfc1 (NM_001139507) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1563804
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214974 representing NM_001139507 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCTGTGTCCCCCGCTAACTTGCCAGCGGTGCTTCTACAGCCCCGCTGGAAACGGGTGGTGG GTTGGTCGGGTCCCGTGCCGCGACCCCGCCACGGCCACCGTGCAGTAGCTATCAAGGAGCTTATAGTGGT GTTTGGCGGCGGCAATGAGGGGATAGTGGACGAACTACACGTGTACAACACTGCGACCAACCAGTGGTTC ATCCCAGCTGTGAGAGGGGATATCCCTCCAGGGTGTGCAGCCTATGGCTTTGTATGTGATGGTACTCGCC TGCTGGTGTTTGGTGGAATGGTAGAGTATGGAAAATACAGCAACGACCTCTATGAGCTCCAGGCAAGTCG CTGGGAATGGAAGAGACTGAAGGCAAAGACACCCAAAAACGGGCCTCCTCCGTGTCCTCGGCTTGGACAC AGTTTCTCCCTTGTGGGCAACAAATGTTACCTGTTTGGGGGTCTGGCCAATGATAGTGAGGACCCCAAGA ACAACATTCCGAGGTACCTGAATGACTTATATATTCTCGAACTACGGCCAGGCTCTGGAGTGGTAGCTTG GGACATTCCCATCACTTATGGTGTCCTTCCTCCACCCCGGGAGTCACATACTGCTGTGGTCTACACTGAA AAAGATAACAAGAAATCCAAGCTGGTGATCTATGGAGGGATGAGTGGCTGCAGGCTAGGGGACCTTTGGA CCCTGGACATTGAGACACTGACATGGAATAAGCCCAGCCTTAGTGGGGTGGCACCCCTTCCTCGCAGCCT CCACTCTGCAACCACCATAGGAAACAAAATGTATGTATTTGGTGGCTGGGTGCCCCTTGTCATGGACGAT GTCAAAGTGGCCACACACGAGAAGGAATGGAAGTGTACCAACACACTGGCTTGTCTCAACCTGGATACCA TGGCCTGGGAAACCATCCTGATGGATACACTGGAGGACAACATTCCTCGAGCTCGAGCAGGCCACTGTGC TGTTGCCATCAATACTCGCCTGTATATTTGGAGTGGCCGTGATGGCTATCGCAAGGCCTGGAACAATCAG GTCTGCTGCAAGGACCTGTGGTATCTGGAGACAGAAAAGCCACCACCCCCAGCCCGAGTTCAACTAGTAC GAGCCAACACCAACTCACTGGAGGTTAGCTGGGGTGCAGTGGCAACAGCCGACAGTTACCTTCTACAACT CCAGAAATATGACATTCCTGCCACAGCTGCTACGGCTACCTCCCCCACTCCCAATCCAGTCCCGTCTGTG CCTGCCAACCCTCCCAAGAGCCCTGCGCCAGCAGCAGCTGCACCTGCTGTACAACCACTGACCCAAGTAG GCATCACACTTGTGCCCCAGGCTGCCGCTGCACCCCCAAGCACAACCACCATCCAGGTCTTGCCGACAGT GCCAGGCAGCTCTATTTCTGTGCCCACTGCAGCCAGGACTCAAGGTGTCCCTGCTGTTCTCAAAGTGACT GGTCCTCAAGCTACAACAGGAACACCACTGGTCACCATGAGACCTGCAAGCCAGGCTGGAAAAGCTCCTG TCACTGTGACTTCCCTGCCTGCCAGTGTGAGAATGGTTGTACCCACTCAGAGTGCCCAGGGCACGGTGAT TGGCAGCAACCCACAGATGAGTGGGATGGCTGCATTGGCTGCTGCTGCTGCTGCCACACAGAAAATCCCT CCTTCCTCAGCACCCACAGTGCTAAGTGTCCCAGCAGGCACCACCATTGTCAAGACAGTGGCTGTGACAC CTGGTACAACCACTCTTCCAGCCACTGTGAAGGTGGCCTCCTCACCTGTTATGGTGAGCAACCCAGCTAC TCGAATGCTAAAGACTGCAGCTGCCCAAGTGGGGACATCTGTGTCCTCTGCTGCCAACACATCTACCCGC CCTATCATCACAGTACATAAATCAGGGACTGTAACAGTGGCCCAGCAAGCCCAGGTGGTGACCACGGTGG TAGGCGGAGTCACCAAGACCATCACTCTAGTGAAGAGCCCCATCTCTGTCCCGGGAGGCAGTGCTCTGAT TTCCAATCTTGGAAAAGTGATGTCAGTGGTCCAGACCAAACCAGTTCAGACTTCAGCAGTGACAGGCCAA GCATCTACAGGTCCTGTGACTCAGATCATCCAGACCAAAGGACCCCTGCCAGCAGGGACTATCCTGAAGT TGGTGACATCAGCAGATGGCAAGCCCACAACCATCATCACCACCACACAGGCTAGTGGGGCAGGGACCAA GCCCACTATCCTGGGCATCAGTAGTGTCTCTCCTAGCACCACCAAACCCGGCACAACTACCATCATTAAG ACCATTCCCATGTCAGCAATTATCACCCAGGCAGGCGCCACAGGTGTTACCAGCAGTCCTGGCATTAAGT CCCCCATCACAATTATCACCACCAAGGTGATGACTTCAGGAACAGGAGCACCTGCCAAAATCATCACTGC TGTCCCCAAGATTGCTACTGGCCATGGGCAACAAGGAGTGACCCAGGTGGTGCTAAAGGGGGCCCCTGGA CAGCCAGGCACCATCCTCCGCACTGTGCCCATGGGCGGCGTTCGCCTGGTCACCCCTGTCACCGTCTCCG CTGTCAAGCCAGCTGTCACCACATTGGTTGTGAAGGGCACCACAGGTGTTACAACACTAGGCACAGTGAC AGGCACTGTCTCCACCAGCCTTGCCGGAGCTGGGGCACATAGCACCAGTGCTTCTCTGGCTACACCTATC ACTACTTTGGGCACCATTGCTACTCTCTCAAGTCAGGTGATCAACCCTACTGCTATCACAGTGTCAGCTG CACAGACTACACTAACAGCTGCTGGTGGACTCACCACACCTACAATCACAATGCAGCCTGTCTCCCAGCC TACCCAGGTGACTCTGATCACAGCACCTAGCGGGGTTGAGGCCCAACCTGTACATGACCTTCCTGTGTCC ATTTTGGCCTCACCTACTACAGAGCAACCCACAGCAACAGTCACTATCGCTGACTCAGGCCAGGGTGATG TACAACCTGGCACTGTGACACTGGTGTGTTCCAACCCACCCTGTGAAACCCATGAAACAGGCACCACCAA CACAGCTACCACCACTGTTGTGGCTAACCTTGGGGGACATCCTCAGCCTACCCAGGTGCAGTTTGTTTGT GACAGACAGGAGGCAGCTGCTTCACTTGTGACCTCAGCTGTGGGACAACAGAATGGTAATGTGGTCCGTG TCTGTTCAAACCCCCCCTGTGAAACCCATGAGACAGGCACCACCAACACTGCCACAACAGCCACCTCAAA CATGGCTGGGCAGCATGGCTGCTCGAATCCTCCCTGCGAGACTCATGAGACAGGTACCACCAGCACTGCC ACTACAGCAATGTCCAGCATGGGCACTGGGCAGCAGCGAGACGCTCGTCGTGCCACTAACACCCCCACTG TAGTGCGGATCACTGTGGCTCCTGGGGCATTGGAAAGAGCCCAGGGTACTGTGAAGCCTCCGTGCCAAAC CCAGCAGACCAACATGACCAGCACCACCATGACTGTGCAGGCCACTGGAGCTCCATGCTCAGCTGGCCCA CTGCTCAGGCCAAGTGTGGCACTGGAGACTGGGAGCCATAGCCCTGCCTTCGTGCAACTAGCCCTTCCAA GTGTCAGAGTTGGGCTAAGTGGCCCCAGCAGCAAGGACGTGCCCACAGGGCGCCAACCAGAGACATATCA TACTTACACAACCAATACCCCAACCACAGCCCGTTCTATCATGGTTGCTGGGGAGCTTGGTACAGCTCGG GTGGTCCCCACATCTCAATATGATTGCCTCCAGGCAAGCTCTCCCAGTAGCACCATGACTATGACAGCCC TAGAGGCCCTGCTGTGCCCTTCAGCTACTGTGACCCAAGTCTGCTCCAACCCACCATGTGAGACCCATGA GACGGGTACCACCAACACCGCCACTACCTCCAATGCGGGCAGTGCTCAGCGAGTATGCTCTAACCCACCT TGTGAGACTCATGAGACGGGCACCACACACACAGCTACCACTGCCACATCAAATGGAGGCGCAGGCCAGC CTGAGGGTGGACAACAGCCTGCCAGTGGCCATCCCTGCGAGACACACCAGACCACTTCCACTGGCACCAC TATGTCAGTCAGTGTGGGTGCCCTGATTCCTGATGCCACTCCCTCTCATGGGACCCTGGAGTCTGGTTTA GAGGTGGTAGCAGTGCCCACTGTCACCTCCCAGGCTGGTGCCACATTGCTGGCCTCTTTCTCAACACAGA GGGTATGCTCCAATCCTCCTTGCGAGACCCACGAGACAGGTACAACGCACACAGCCACCACTGTCACCTC TAACATGAGCTCAAACCAAGACCCTCCACCAGCTGCCAGTGACCAAGGAGAAGTGGCGAGTACCCAAGGT GACAGCACAAATATCACCAGTGCCAGTGCTATCACTACAACTGTGTCTTCTACACTGCCAAGAGCAGTGA CCACTGTGACACAGTCTACACCAGTCCCAGGTCCTTCTGTGCCGCCCCCAGAGGAACTCCAGGTCTCACC AGGGCCTCGCCAGCAGCTGCCTCCAAGGCAACTCCTGCAGTCTGCCTCCACACCCCTGATGGGGGAGTCC GCCGAGGTCCTGTCAGCCTCCCAGACCCCTGAGCTCCAGGCCGCCGTGGATCTGAGCAGCACTGGGGACC CATCTTCAGTCCAGGAGCCTACCACCTCTGCTGTTGTGGCCACTGTGGTGGTCCAACCACCCCAACCCAC ACAGTCTGAAGTAGACCAGTTATCACTTCCCCAAGAGCTGATGGCTGAAGCCCAGGCGGGCACCACAACC CTTATGGTAACAGGGCTCACTCCAGAGGAGCTGGCAGTGACTGCTGCTGCTGAAGCAGCTGCTCAAGCTG CAGCCACTGAAGAAGCTCAAGCCTTGGCCATCCAGGCTGTGCTCCAGGCTGCACAGCAGGCTGTCATGGG CACTGGGGAGCCCATGGATACATCTGAAGCAGCAGCAGCAGTGACACAAGCAGAACTGGGTCATCTTTCA GCTGAAGGCCAAGAGGGTCAGGCTACCACCATACCCATTGTGCTGACACAGCAGGAGCTTGCAGCTCTTG TACAGCAGCAGCAGCAGCTCCAGGAGGCTCAAGCCCAAGCCCAGCAACAGCACCACCTTCCCACTGAGGC TCTGGCCCCAGCTGACAGTCTCAATGACCCATCCATCGAGAGCAACTGCCTCAATGAGTTAGCTAGTGCT GTCCCCAGCACTGTGGCCTTGCTACCCTCAACAGCTACTGAGAGTTTGGCTCCATCTAACACATTTGTGG CTCCTCAGCCTGTTGTTGTAGCCAGTCCAGCAAAGATGCAGGCTGCAGCAACCCTTACTGAAGTGGCAAA TGGCATTGAGTCCCTGGGTGTGAAACCGGACTTGCCACCCCCACCCAGCAAAGCCCCTATAAAAAAGGAG AACCAGTGGTTTGATGTGGGGGTCATTAAAGGTACCAGTGTAATGGTGACACACTATTTTCTGCCACCAG ATGATGCTGTTCAGTCAGATGATGACTCAGGCACCGTTCCAGACTATAACCAGCTAAAGAAGCAGGAGCT GCAGCCAGGCACAGCTTATAAATTTCGAGTTGCTGGAATCAATGCTTGTGGCCGGGGCCCCTTCAGTGAG ATCTCAGCCTTTAAGACTTGTCTGCCTGGTTTTCCAGGGGCTCCTTGTGCTATTAAAATCAGCAAGAGCC CAGATGGTGCTCACCTCACCTGGGAGCCACCCTCTGTGACCTCCGGCAAGATTATCGAGTACTCTGTGTA CCTGGCCATCCAGAGCTCACAGGCCAGTGGTGAGCCCAAGAGCTCTACCCCAGCCCAGCTGGCCTTCATG CGAGTATACTGTGGGCCTAGCCCTTCCTGCCTTGTGCAGTCCTCCAGCCTCTCCAACGCCCACATTGACT ATACTACAAAGCCTGCCATCATCTTCCGCATTGCTGCCCGCAATGAAAAGGGCTACGGCCCTGCCACACA AGTGAGGTGGTTACAAGAAACCAGTAAAGACAGCTCTGGCACCAAGCCAGCTAGCAAGCGGCCCATGTCG TCTCCAGAAATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001139507
Insert Size	6105 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001139507.1, NP_001132979.1
RefSeq Size	9128 bp
RefSeq ORF	6105 bp
Locus ID	363519

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