Plcb3 (NM_033350) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214977 representing NM_033350 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC GCGGGAGTAAATTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGAGTGGACCC CAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCCAAGATTCGAGAGGTACTGGGCTTTGGGG GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTAGCAGGACCAGATCCAGTAAACACCACATT CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAGCTGGCTATG AACATACTGGCTCAGAACGCCTCCCGGAACACGTTCCTTCGGAAAGCATACACGAAACTGAAGCTGCAGG TGAACCAGGATGGACGGATTCCAGTCAAGAACATTCTGAAGATGTTCTCCGCGGACAAGAAGCGGGTGGA GACTGCACTGGAGTCCTGCGGCCTCAACTTCAACCGGAGTGAGTCCATTCGGCCTGATGAGTTTTCCTTG GAAATCTTCGAGCGGTTCTTGAATAAGCTGTGTCTGCGACCGGATATTGACAAGATCCTGCTGGAGATAG GTGCTAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAAAGAGACCC AAGACTCAACGAGGTGCTGTACCCACCGCTTCGCTCCTCACAGGCTCGGCTGCTCATTGAGAAGTATGAG CCGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGTTTCAGCCGCTATCTGGGAGGGGAGG AGAATGGCATCCTGCCCCTGGAGGCCCTGGATCTGAGCATGGACATGACGCAGCCACTGAGCGCATACTT CATCAATTCTTCACACAATACCTATCTCACTGCGGGCCAGCTGGCTGGAACATCGTCAGTGGAGATGTAC CGCCAGGCGCTGCTGTGGGGCTGCCGTTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG AGCCCTTCATTACCCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGTTAGAGGCCATCGC CGAGACAGCCTTCAAGACCTCACCCTACCCTGTCATTCTCTCCTTCGAAAACCATGTGGACTCGGCAAAA CAGCAGGCCAAGATGGCAGAGTACTGCCGCTCTATCTTTGGGGAAGCGCTGCTCATTGACCCACTGGACA AGTACCCGCTGTCTGCGGGCACCCCCCTTCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA TAAGAAGCGGCATCGGCCCAGCACAGGTGTCCCTGACAGCTCCGTGCGCAAGCGGCCTCTGGAACAGAGC AATTCAGCCCTGAGTGAGAGCTCAGCTGCCACAGAGCCCTCCTCACCCCAGCTGGGGTCCCCCAGCTCTG ACAGCTGCCCTGGCCTAAGCAATGGGGAAGAGGTGGGACTTGAGAAGACCAGCCTGGAGCCTCAGAAGTC TCTAGGTGAGGAGGGCCTCAACCGAGGACCTAATGTTCTCATGCCTGACCGTGACCGAGAGGATGAGGAG GAAGATGAAGAAGAGGAGGAAACAACGGATCCAAAAAAGCCCACCACCGATGAGGGCACAGCCAGCAGTG AGGTCAACGCCACAGAGGAGATGTCGACACTCGTCAATTACGTTGAGCCCGTCAAGTTCAAGTCCTTTGA GGCTGCTCGAAAAAGGAACAAGTGCTTTGAGATGTCGTCCTTTGTGGAGACCAAGGCGATGGAGCAGCTG ACCAAGAGCCCCATGGAGTTCGTGGAATACAACAAACAGCAGCTTAGTCGCATCTACCCCAAGGGCACCC GTGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGCTGTCAGCTTGTTGCCCTCAACTT CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAACGGGCGCAGTGGGTACTTG CTCAAGCCTGAGTTTATGCGGCGGCCGGACAAGTCTTTTGACCCCTTCACTGAGGTCATCGTAGATGGCA TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTTCTGTCTGACAGGAAGGTGGGCATCTA TGTCGAGGTGGACATGTTTGGTCTCCCAGTTGACACAAGACGCAAATATCGCACACGGACATCCCAGGGG AACTCATTCAACCCTGTGTGGGATGAAGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT CACTTCGCATTGCAGCCTTTGAGGAGGGTGGCAGATTTGTTGGGCATCGTATCCTGCCTGTCTCTGCTAT CCGCTCAGGATACCACTACGTCTGCCTGCGAAATGAGGCCAACCAACCGTTGTGCCTGCCTGCCCTGCTT ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATCAACCCCA TCAAGCACGTCAGCCTAATGGACCAGCGGGCCAAGCAACTGGCTGCTCTCATTGGGGAGAGTGAGGCTCA GGCCAGTACAGAAATGTGTCAGGAGACCCCGTCTCAACAGCAAGGCTCACAGCTCTCCTCCAACCCCGCA CCTAACCCACTGGATGCCTCACCTCGATGGCCCCCAGGTCCTACCACCTCCCCCACTAGCACCTCCCTCA GTAGCCCAGGGCAGCGAGATGATCTGATCGCCAGCATCCTTTCTGAGGTGACCCCAACACCCCTAGAGGA GCTCCGAAGCCACAAGGCTATGGTGAAGCTCCGGAGCCGGCAGGATCGAGACCTGAGGGAACTGCATAAA AAACACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG GGAAGTGCCGGCCCTCCTCCAGCGCCCTGAGCAGGGCCACCAATGTGGAAGACGTGAAGGAGGAAGAAAA AGAGGCAGCCAGACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGCTGGAGCTGAGGGAGGCC CAGGCAGATGCAGAGACAGAACGGAGGCTGGAGCATCTGAAACAGGCTCAGCAGCGGCTCAGGGAGGTTG TCCTGGACGCACATACGACTCAGTTCAAGAGGCTGAAGGAGTTGAATGAAAGGGAGAAGAAGGAGCTTCA GAAGATCCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAG GTGGAACTGACAGAGATCAATCGGAGGCACATCACTGAGTCAGTGAACTCCATCAGGAGGCTGGAAGAGG CCCAGAAGCAGCGCCACGAACGCCTCTTGGCAGGGCAACAGCAGGTCCTCCAGCAGCTAGTGGAAGAGGA GCCCAAGCTGGTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGCTGCCCCAGGAGATCCGT CGGTGCCTGCTGGGCGAGACATCAGAGGGATTGGGGGATGGCCCCCTGGTGGCCTGTGCCAGCAATGGTC ATGCAGCTGGGAGTGGTGGGCACCAGTCCGGCGCTGACTCGGAGAGCCAGGAGGAAAACACCCAGCTTTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_033350
Insert Size	3711 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_033350.1, NP_203501.1
RefSeq Size	3726 bp
RefSeq ORF	3711 bp
Locus ID	29322
Gene Summary	phospholipase C isozyme activated by Gq class G proteins [RGD, Feb 2006]

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