Anapc1 (NM_001107771) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215153 representing NM_001107771 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATCTGCAGGAATTCGTTCCTTTTG GTCGAGACCACTGCAAGCACCACCCTAATGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCTTCTGA GTTATGGTCTTCCGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACGATCCATGAGAAACAG AAGGAAAGCTGGCAGTTAAGAAAAGGCGTGAGCGAGATTGGAGATGCAGCCGACTATGATGAAGAGCTCT ATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAAAGCCAGGCCTTGGCAGTGTACAAAGCCTT CACAGTGGACAGCACTGTTCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCCGAAAAG ATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGCTCGTGTATGAACATGCACAGCA TAGACGGGAAGGATTACATAGCTTCCTTACCATTTCAGGTGGCAAACGTGTGGGCCACTAAGTATGGCTT GCTGTTTGAACGAAGCAGTTCTTCACATGAGGTGCCTCCAAGTCTACCTAGAGAGCCTTTGCCTACCATG TTCAGCATGCTGCACCCTCTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCAT CGCGGGTGCAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTAAT GACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTCAGGAGGGTAAAGCCAGAGGAAGAGACT GCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAGCCACTAGCAGCTCCCTGACAGCAC ATCTCAGAAGCCTCTCCAAAGGCGAGTCCCCTGTTGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAG CCAGAGCCGCTCAGCCTCCTCACCCAGCCTACACTCCCGGTCGCCTTCCATTTCCAACATGGCGGCTCTA AGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGTGCAAAGATTCAACCTTTCAAGCC ACAATCAGTCACCAAAGAGACACAGTATTTCCCACTCTCCGAGTGGCAGTTTCAACGATTCATTTTTGGC ACCAGAAACAGAGCCAATTGTTCCTGAGCTTTGCATTGACCACTTGTGGACTGAGACACTTCCGAATATG AGAGAGAAAAATTCTCAGGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCCTGTGCT TTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTAT CTTTGGCTCTGTCACCAACATACATGCAAAAGACGCAGCCCCAGTGGAGAAGATAGACACCATGTTGGTA CTCGAAGGCAATGGGAATCTGGTGCTGTACACAGGAGTGGTTCGGGTGGGGAAAGTGTTTATTCCTGGAC TTCCAGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCACTCGATGGTGTTAGCAC TCCGAAGCCTCTTAGCAAATTACTTGGATCAATGGATGAGGTGGTTCTTCTGTCTCCAGTTCCAGAATTG AGGGATTCTTCAAAACTTAACGATTCTCTCTACAATGAGGATTGTACATTCCAGCAGCTTGGAACTTACA TTCATTCAGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAACGGCTCCATGGTTAGGAT CACTATCCCTGAAGTGGCCACCTCGGAATTAGTGCAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCG AAAGAAGTAGCAGTTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCGGGAGGACCCAGTTGTC ACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATG GACGCGAAGTTTCGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCGAGGCCCTCTGAT ACTGGGTCTGACGATGACTGGGAGTACTTACTGAATTCAGAGTACCATCATAATGTTGAGTCTCATCTTT TGAACAAATCTCTATGTTTGAATGCTTTGGAAGTTACAAAGACAAAAGATGAAGATTTTCCACAAAACCT CAGTCTGGATTCTTCTACTCTTCTCTTTGCTCACATTCCTGCAATTTTTTTTGTTCTTCACCTGGTTTAT GAAGAGCTTAAGTTAAATACTCTAATGGGAGAAGGAATTTGTTCTCTAATTGACCTCCTTGTTCAGTTGG CAAGGGACTTAAAATTGGAGCCTTACTTGGACCATTACTACAGAGATTACCCAACTCTTGTCAAAACTAC TGGACAAGTGTGTACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCTCATTTTTTACTTCCGAG CCACCAAGTATTTATCAGTGGGTGAGTTCGTGCCTGAAGGGCGAAGGAACGCCACCCTATCCCTACCTCC CTGGGATCTGCGAGAGGAGCAGGCTGGTGGTCTTGAGTATTGCCCTCTACACCCTTGGTGATGAGAGTTG TGTTTCTGATGAAACCTCCCAGTATTTATCCAAAGTAACCTTAACTCCCCAAAAGCCACAAGCAGAACAA GAAGAAAACAGGTTTACTTTCCGGCAGTCTGCTTCTGTGTCTGTTCTGGCTGAGAGACTGGTTGTCTGGA TGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGA TGCAATCTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCCGTCTGTCTCCTGATTGGACGT CAGGACCTTTCCAAGCAGGCTTGCGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGATGTGG CTTCAGGAGCTGAGGCTGAGGAGGAAGACGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAAT ATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTACTTCAAAGTGCACAGCCTGTCCGTGTCAAT GTGGTACAGTACCCAGAACTCAGTGACCATGAGTTCATTGAGGAAAAAGAAAACAGGCTGCTCCAGTTGT GTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGGATGTTTACCTTGTTCTCATACCATCCTGTTCCAAC AGAGCCGTTGCCCATTCCTAAATTGAATCTGACAGGGCGAGCCCCTCCGAGAAACACAACTGTAGATCTT AATAGTGGGAACATCGATGTGCCTCCCAATATGGCAAGTTGGGCCAGCTTTCATAATGGTGTGGCTGCTG GCCTGAAGATAGCCCCTGCCTCCCAGATAGACTCAGCTTGGATTGTTTACAACAAGCCAAAGCATGCTGA GTTAGCCAATGAGTACGCAGGCTTTCTCATGGCCCTAGGTCTGAATGGGCACCTTACCAAGCTGGCTACT CTCAATATCCATGACTACTTGACCAAGGGCCATGAAATGACAAGCATTGGATTGCTACTTGGTGTTTCTG CTGCAAAACTTGGCACCATGGACATGTCAATTACCCGGCTTCTTAGCATTCACGTTCCTGCTCTCTTACC CCCGACATCCACAGAGCTTGATGTGCCTCACAATGTCCAAGTGGCGGCTGTGGTTGGCATTGGCCTTGTT TATCAAGGCACAGCTCACAGACATACTGCAGAAGTCCTGTTGGCTGAAATAGGGCGGCCCCCTGGTCCAG AAATGGAATATTGCACTGACAGAGAATCCTACTCCTTAGCTGCTGGCCTGGCCCTGGGCATGGTTTGCTT GGGGCATGGCAGCAATTTGATTGGCATGTCTGACCTCAATGTGCCTGAGCAGCTGTATCAGTACATGGTC GGAGGTCACAGGCGTTTTCAAACTGGAATGCACAGGGAGAAACATAAGTCTCCAAGTTACCAGATCAAAG AAGGAGATACCATAAACGTGGACGTGACTTGTCCAGGCGCTACTCTGGCTCTGGCTATGATCTACTTGAA AACCAATAACAGATCTATTGCTGATTGGCTGCGAGCTCCTGATACCATGTATTTGCTCGACTTTGTGAAA CCAGAGTTCCTCTTGCTTAGGACACTTGCTCGGTGCCTGATCCTGTGGGATGACATTTTACCGAATTCCA AGTGGGTTGACAGCAATGTTCCTCAGATTATAAGAGAAAATAGTATCTCTCTGAGTGAGATTGAATTGCC TTGTTCAGAGGACTTGAATTTGGAAACCTTGTCGCAAGCACACGTCTACATCATCGCAGGAGCCTGCTTG TCTCTGGGTTTTCGATTTGCTGGCTCAGAGAACTTATCAGCATTTAACTGTCTGCATAAATTTGCAAAAG ATTTTATGAATTATTTATCTGCACCCAATGCTTCTGTAACAGGGCCCTACAACCTCGAAACCTGCCTGAG TGTTGTCCTGCTGTCTCTTGCCATGGTGATGGCTGGCTCTGGGAACCTGAAGGTGTTGCAGCTCTGCCGC TTCCTGCATATGAAGACGGGCGGAGAGATGAACTATGGCTTCCACTTGGCCCACCACATGGCCCTGGGCC TTCTCTTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCCAACTCATCCATTGCTGCCCTTCTCTGTGC CCTTTACCCACATTTCCCAGCCCACAGCACTGACAACCGGTATCATCTCCAGGCCCTTCGGCACCTCTAC GTGCTGGCTGCAGAACCAAGGCTCCTGGTGCCTGTGGATGTGGATACAAACACACCCTGCTATGCCCTTA TAGAAGTTACTTACAAGGGCACTCAGTGGTATGAACAGACCAAAGAAGAACTGATGGCTCCAACCCTGCT TCCAGAACTCCATCTTCTAAAGCAGATGAAGGTTAAAGGGCCAAGATACTGGGAACTGCTCATAGATTTA AGCAAGGGAGAGCAGCACTTGAGGTCCATTCTTTCCAAGGATGGAGTTTTATATGTAAAGCTCAGGGCAG GCCAGCTCTCCTACAAAGAAGACCCAATGGGGTGGCAGAGTCTGTTGGCACAGACTGTGGCTAACAGGAA CTCAGAGGCCCGGGCTTTCAAGCCAGAAACAATTTCATCATTCACTTCTGATCCAGCACTTCTGTCATTT GCTGAATATTTCTGCAAGCCGACTGTGAGCATGGGCCAAAAACAGGAGATTCTGGACCTCTTTTCTTCCA TACTTTATGAGTGTGTTGCTCAGGAAACTCCAGAGATGTTGCCTGCCTACATAGCAATGGATCAGGCTTT AAGAAGTCTTAAAAAGAGAGACATGTCGAACACATCTGATCTTTGGCAGATAAAGTTGATATTAGAGTTT TTCAGTTCACGAAGCCACCAGGAAAGGCAGCAGACCTATCCTAAGCGAGGGCTCTTCATAAACTCGGAGT TCCTACCTGTGGTGAAGTGCACTGTTGATTCCACCCTGGACCAGTGGCTACAAGCTGGAGGCGATGTGTG TGTGCATGCCTACCTTAGTGGGCAGCCTGTTGAGAAGTCTCAGCTAAACATGCTGGCCTGCTTCCTGGTC TACCACTCTGTGCCAGCACCGCGACACTTGCCACCCCTGGGACTGGAAGGGAGCACAAGCTTTGCTGAAC TCCTCTTCAGGTTCAGACACCTAAAAATGCCAGTCCGCGCTTTGCTGAGACTGGCTCCTGTGCTGCTCGG GAATCCACAGCCGATGGTTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107771
Insert Size	5835 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107771.1, NP_001101241.1
RefSeq Size	8895 bp
RefSeq ORF	5835 bp
Locus ID	311412

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.