Helz (NM_001105848) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215156 representing NM_001105848 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGACAGAAGAGCTGAAAGGTCATGTGAACAAGCGTGCGCGTCACTGCAGAGGCAGGACTATGACA TGGCCCTCCAGCATTGCACAGACGCCCTGCTCTCTCTTGGCCAGTACTCCATGACAGACTTCGCAGGGCC GTGCCCAGTGGAGATTGAGCGCATCAAAATCGAGAGTCTTCTGTACAGAATTGCCTCATTCTTGCAGTTG AAAAACTACGGGCAAGCTGATGAAGATTGTAGGCATGTGCTGGGGGAAGGACTGGCCAAGGGAGAGAGCG CCTTCCGGGCAGTGCTGTGCTGCATGCAGCTGAAGGGGAAGCTCCAGCCTGTGTCCTCCATCCTTGCCCA GTCACTGTCAGGGGAGTCTCTGAATGGGATGGTAACGAAGGATTTGACAAGACTCAAGACACTTCTCGCA GAGACAGAGACAGCAACAAGTAATGCCCCCTCTGGCTGTCATGTGGAAGACTTAGATGAAGGGTCTTGTA ATGGTTGGCATTTCCGCCCACCGCCCAGGGGGATCACCAGCAGTGAGGAGTACACCTTGTGTAAAAGGTT TTTAGAGCAAGGGATCTGTAGGTACGGTGCCCAGTGTACTTCAGCACATTCCCAGGAAGAACTAGCAGAA TGGCAGAAGAGATATGCCTCGCGGCTGATAAAACTGAAGCAGCAGAGTGAGAACAAACAGCTCTCAGGCA GCTACATGGAGACCTTGATAGAAAAGTGGATGAGCTCACTGTCCCCTGAGAAAGTGCTTAGCGAATGCCT AGAGGGAGTCCAGGTGGAGCACAGCCCTGACCTGTCAGTCACCGTCAACACCAAAAAGTCCCACCAGACA TGGACCTTTGCTCTGACCTGTAAGCCTGCAAGAATGCTGTGTCGGGTAGCCTTGCTCTATGATGCTCATC GTCCTCATTTTAGTATCATTGCCATATCTGCCGGAGATAGTACTGCCCAGGTATCACAAGAAGTCCCCGA GAACTGTCAAGAATGGATAGGAGGAGAGATGGCCCAAAATGGATTAGATCATTACGTGTATAAAGTCGGG ATAGCATTTAACACAGAAATATTTGGAACTTTTCGCCAAACCATAGTTTTCGACTTTGGATTGGAGCCAG TACTCATGCAAAGAGTAATGATTGACGCAGCTTCTACAGAAGATCTTGAGTACCTGATGCATGCCAAGCA GCAGCTGGTAACCACAGCCAGGCGCTGGGAGCCTTCCTCCAAGACTATTGTAGACTTTGAACCTAACGAG ACTACTGATTGGGAGAAGAGCCTTTTGATCAGATACCAAATTCCTCTCTCTGCTGACCAGCTGTTTACAC AGTCCGTTTTAGACAAATCCTTGACCAAGACTAACTATCAGTCACGGCTACACGACCTGCTTTATATTGA GGAGATAGCCCAGTATAAAGAAGTCAGCAGGTTCAACCTTAAGGTGCAGCTGCAGATCTTAGCAAGCTTC ATGCTCACTGGAGTTTCTGGGGGTGCAAAGTATGCTCAGAATGGACAACTTTTTGGTCGTTTTAAACTTA CTGAAACCCTGTCTGAAGACACTTTGGCTGGACGACTGGTGATGACCAGGGTCAATGCTGTTTATTTATT ACCAGTCCCTAAAGAGAAGCTCGTCCAGTCCCAGGGAACCAAAGAGAAGGTTTATGAAGCTACTATTGAA GAAAAAACAAAAGACTATGTATTTTTAAGGATATCCAGGGAATGCTGTGAAGAGCTCAGTCTGCGGCCTG ACTGTGACACCCAGGTTGAGCTTCAGTTTCAGTTGAACCGATTACCCCTCTGTGAAATGCATTATGCACT AGACAGGATTAAGGACAATGGTGTTTTGTTTCCAGACATCAGTATGACTCCCACTATACCTTGGAGTCCC AACAGACAGTGGGATGAGCAGTTGGATCCTCGACTAAATGCAAAGCAGAAAGAAGCTGTGCTGGCCATCA CCACTCCGCTCTGCATCCAGCTGCCCCCCGTTCTTATCATTGGACCCTACGGGACGGGTAAAACCTTCAC TCTGGCTCAGGCCGTCAAGCACATACTGCAGCAGCAGGAGACGAGCAGGATTCTCATTTGCACCCATTCT AATAGTGCTGCTGACCTCTACATAAAGGATTATTTACATCCCTATGTAGAAGCAGGCAATGCCCAGGCAA GACCTCTCAGGGTGTATTTCCGAAATCGCTGGGTGAAGACTGTTCACCCGGTTGTGCATCAGTACTGTTT GATCTCAAGCGCACACTCCACCTTTCAGATGCCCCAGAAGGAAGATATTCTTAAACATCGAGTGGTGGTG GTCACACTGAACACTTCCCAGTACCTCTGCCAGCTGGACCTTGAACCTGGGTTCTTCACGCACATTCTGT TAGATGAAGCTGCCCAGGCCATGGAGTGTGAGACCATTATGCCTCTAGCCTTAGCAACTAAAAACACACG GATTGTTTTGGCTGGTGACCATATGCAGCTCAGTCCTTTTGTTTACAGCGAGTTTGCCAGGGAGAGAAAC CTTCATGTTTCATTACTTGATCGACTCTACGAGCATTACCCTGCCGAGTTTCCATGCAGGATCCTCCTCT GTGAGAACTATCGCTCCCATGAAGCTATCATCAACTATACCTCTGAGCTCTTCTATGAGGGTAAACTGAT GGCCAGTGGAAAGCAGCCAGCACACAAGGACTTCTACCCACTGACGTTCTTTACAGCACGAGGGGAGGAC GTCCAAGAGAAGAACAGCACGGCATTCTATAACAATGCAGAGGTATTTGAAGTGGTGGAGCGTGTGGAAG AGCTAAGACGGAAGTGGCCAGTGGCATGGGGGAAGTTAGACGACGGCAGCATTGGTGTGGTGACACCATA CGCTGATCAGGTGTTCAGAATACGAGCTGAACTTCGGAAGAAGAGGCTCTCGGACGTGAATGTAGAAAGG GTGCTCAATGTCCAAGGAAAGCAATTCAGAGTTCTGTTTCTTAGCACAGTGCGTACACGCCACACTTGCA AGCATAAGCAGACGCCAATTAAAAAGAAAGAGCAACTTCTGGAAGACTCCACCGAGGACTTAGATTACGG GTTTCTATCCAACTACAAACTTCTCAATACCGCCATTACCAGAGCACAGTCCCTGGTTGCTGTGGTGGGC GACCCCGTTGCTCTGTGCTCTATTGGAAGGTGTAGAAAGTTTTGGGAGCGGTTTATTGCCCTGTGTCATG AAAACCACAGCTTGCACGGGATCACATTCGAACAGATCAAGGCCCAGTTAGAGGCTTTAGAGCTCAAGAA GACTTACGTGTTGAACCCACTGGCTCCTGAATTTATTCCCCGGGCTTTCAGACTGCAGCATTCAGGAAAC AGTAGCAGACAGCAGCAGTCACCACCCAAGGTGAAAAGCCTCCATCATCCCCAGAATGACCATTTCCAGA ATGATGGAATTGTTCAGCCCAACCCTTCCGTACTTATTGGCAATCCTATTAGAGCATATACTCCTCCACC CCCTCTTGGACCGCACCCAAACTTGGGGAAATCTCCAAGCCCCGTTCAAAGAATAGACCCTCACACTGGG ACTAGTATTCTTTACGTTCCTGCTGTCTATGGAGGGAATGTAGTTATGTCGGTGCCTTTACCTGTGCCGT GGACAGGGTACCAGGGTAGGTTTGCAGTTGATCCTCGGATTATTACACACCAGGCAGCGATGGCCTACAA TATGAATTTGTTACACACACATGGGCGTGGGTCGCCAATCCCTTATGGTCTTGGACATCACCCACCTGTC AACCTAGGCCAGCCACAGAATCAGCACGCAGAGAAGGATCAACAGGAGCAAAATCGAAACGGTAAAACTG ATACAAATAACCCAGGACCTGAAATTAATAAGATCCGAACACCAGAGAAAAAACCTACAGAACCCAAACA GATTGATTTGGAATCAAATCCACAGAACAGAAGTCCTGAATCCCGTCCTGGTGTTGTTTATTCCAATACT AAATTTCCTCGAAAAGATAATCTCAACCCAAGACACATAAATCTTCCTCTTCCTGCGCCACATACACAGT ATGCAATCCCTAGCCGCCATTTTCATCCGCTTCCTCAGCTACCCAGGCCACCATTTCCCATTTCCCAGCA GCACACCTTATTAAATCAGCAGCAGAATAATTTGCCTGAACAACCAAATCAGATGACACCTCAGCCAAAT CAGGTGGCACCCCAGCCAAATCAGATGGCACCCCAGCCAAATCAGATGGCACCCCAGCCAAATCAGGTGG TCCAGCAGCAGAACCAGGCACCTCCACAGGCTCCACAGCCAGCACCTCAACTCTCTCCTGCATTTCAGGC GGGGCCCACCAATGCATTTTTCAATAATGCAGTTGCTCATCGACCACAGTCTCCTGCTGCAGAGGCTGTG GGTCCTGAGCAGCAGGTGCCTCCTGGGCTGTCCGACGGCCACAGTCCACTGAGAGCCATCACACAGCCTG GCCCCATCCTAGCATCACCTTTGAACAACTTTGTTGATGAGAGCTCTCCAGGATTGCCTATAGAAGAAGC TTTGGATCGAGTGCATGGAAGTGTAGCTCTGGAGACACTGAGGCAGCAGCAGGCACGGCTACAGCAGTGG AGTGAGCACCATGCCTACCTGAGCCAGGGTGGCATCCCGTATTCACACCATCACCATCCTCACCTCGCCC ATCTTCCCCAGACTCCCATTAGCCTCCACCAGCCACCAGTGAGAGCAGAGTGGAAAGTCGCCGGCAGGGC TGACGATGAAACAGAGACAACATTCTCAAGGTTTCAAGACTTACTCAGAGAGCTGTCCCATCGGGATCAA GGTGACACTGGAGAGCTAGCTGAAATGCCACCGCCTCAGTCAAGACTTCTGCAGTACAGACAAGTGCAGC CTCGAAGCCCACCAGCAGTCCCGTCGCCCCCTTCCTCTACAGACCACAGTAGCCACTTTGCTAACTTTAA TGACAACAGCAGAGACATCGAAGTAGCCAACAGCCCAGCATTCCCACAGCGCCTCCCACCTCAGCTATTC GGCCCCCCTTATTCGTTGCAATCTGAACACCTTGCCCCTCCCCCTCTGAAATACCTGGCTCCGGAAGGAG CATGGAATTTTGCTAACTTGCAGCAGAATCATTTAATAGGGCCGGGCTTTCCCTATGGCCTACCTCCATT GCCTCCCCGGCCACCACAGAACCCTTTTATACACATCCAGAACCATCAACACGCTGTTGGTCAAGAGCCA TTTCACCCACTGCCATCTCGAGCAGTGTCTTCTTCTTCGCTCCCCAGCCTAGAAGAGTATGAGCCCAGAG GACCTGGTCGGCCCTTGTACCAAAGAAGAATCTCATCTGGCGCAGCCCAGCCTTGTGTGGAAGAGGCCAG CACTCCCCAAGACAGCCTGGCTCAGGGCAAAGAACCGCAGGACCACAGTAACCCACCTGGGTTCGGCTTC CCAGCCCCCGAGTCCTGGGCCAACACCACCTCATCTGCCCCCTACCAGAACATTCCATGCAATGGATCCA GCAGGACAAGTCAGCCCAGAGAGTTGATAGTTCCACCCAAGACTGTCAAACCCCCTGAGGATCAGCTGAA GCCTGACAGTGGGGAGGTGTCCAGTTCCTTCAACTACTCGGTGCTGCAGCACCTCGGCCAGTTCCCACCC CTCATGCCCAACAAGCAGATCGCGGAGTCTGCCAACAGCAGCAGCCAGCAGAGCCCGGCAGGGAGCAAGC CCGCCATGTCCTATGCCAGTGCGCTACGGGCCCCGCCCAAGCCCAGGCCTCCGCCCGAGCAGGCCAAGAA GGGCAGCGACCCACTGTCCCTGTTCCAGGAGCTCAGCTTGGGCAGCAGCCCGGGAAGCAATGGCTTCTAC TCCTACTTCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105848
Insert Size	5895 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105848.2, NP_001099318.2
RefSeq Size	6193 bp
RefSeq ORF	5895 bp
Locus ID	287773

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