Arid4b (NM_053421) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Bcaa
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215517 representing NM_053421 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCCTTGATGAGCCTCCTTATTTGACAGTGGGCACTGATGTGAGTGCCAAGTACAGAGGAGCCT TTTGTGAAGCCAAGATCAAGACTGCAAAAAGACTTGTCAAAGTCAAGGTGACTTTTAGACACGATTCTTC AACAGTGGAAGTTCAGGATGACCACATAAAGGGCCCACTGAAGGTAGGAGCTATTGTGGAAGTGAAGAAT CTTGATGGTGCCTATCAGGAAGCTGTTATCAATAAACTGACAGATGCAAGTTGGTATACTGTGGTTTTTG ATGATGGAGATGAGAAGACACTGAGAAGATCTTCATTATGTCTGAAAGGAGAGAGACATTTTGCTGAAAG TGAAACACTAGACCAGCTCCCACTTACCAATCCTGAGCATTTTGGCACTCCAGTCATAGGAAAGAAAACA AATAGAGGAAGAAGATCTAATCATATACCAGAGGAGGAATCTTCTTCCTCATCCAGTGATGACGATGAGG ATGATAGGAAACAGACTGACGAACTGCTAGGCAAAGTTGTTTGTGTAGATTATATTAGTTTGGATAAAAA GAAAGCGCTGTGGTTCCCTGCACTGGTGGTTTGTCCTGACTGTAGTGATGAGATCGCCGTGAAAAAAGAC AATATTCTTGTGCGATCTTTCAAAGATGGCAAATTTACTTCAGTTCCAAGGAAAGATGTCCATGAAATTA CTAGTGACACTGCACCAAAGCCTGATGCCGTATTAAAACAAGCCTTTGATCAGGCACTTGAGTTTCACAA AAGTAGAACTATTCCTGCTAACTGGAAGACTGAACTGAAAGAAGACAGCTCCAGTAGTGAAGCCGAGGAA GAGGAGGAGGAGGAAGATGATGAAAAAGAGAAAGAAGATAATAGCAGCGAAGAAGAGGAAGAAATAGAAC CATTTCCAGAGGAAAGGGAGAACTTTCTTCAGCAGCTGTATAAATTCATGGAGGATAGAGGTACACCTAT TAACAAACGGCCTGTGCTTGGGTATCGAAATCTGAATCTTTTTAAGTTATTCAGACTTGTGCACAAACTT GGAGGATTTGATAACATTGAAAGTGGAGCTGTTTGGAAGCAAGTCTACCAAGACCTTGGCATCCCTGTCT TAAATTCAGCTGCAGGATATAACGTTAAATGTGCTTACAAAAAGTACTTATATGGCTTTGAAGAGTACTG CAGGTCGGCAAATATTGATTTTCAGATGGCATTGCCAGAGAAAGTTCTTAACAAGCCATGTAAAGATTGT GAAAATAAAGAAATAAAAGTTAAAGAAGAAAGTGATGCAGAGATCAAAGAAGTAAATGTGGAAGACAGTA AGAACATGATACCAAAAGAGGAGACGCCTGCTGAGGATGAGAGTGAAAGAAAAGAAAATATCAAACCCTC TCTGGGAAGTAAAAAAGGCTTATTAGAATGTATACCTGCACAATCTGATCAAGAAAAAGAAGCTAACATT ACAAAACTAGAAGAAAAGGAAAGCTTGGAAGATAAAGACGGTGCCACAGCTAGGGCCGAGGAGGCCCTCA GCACAGAGGTAGATGCTGAAGAAGAGCAAGCACGATCTGGATATGATGAATGGATTAAAGCAGATAAAAT AGTAAGACCTGCTGATAAAAATGTACCAAAGATAAAACATCGGAAGAAAATAAAGAATAAACTAGACAAA GAAAAAGACAGAGATGAAAAATACTCTCCTAAGAACTGTAAACTTCGACGCTTGTCAAAATCACCGTTTC AGTCAAATCCATCTCCTGAGATGGTTTCCAAACTAGATCTTGCTGATGCCAAAAATTCTGATACAGGTCA TATTAAATCTATAGAAATTACCTCCATTCTTAATGGACTACAAGCTTCCGAAAGCTCAGCTGAAGACAGT GAGCAGGAGGAGGAGAGGTGTGCTCAGGACCCAGAAAGCAGTAGCAAAGACGAATCAAAGGTGGAACATT CAGCCCACAGCAGAAGTGAGCTGATTTCAAAAGAGGAACTGAGTAGTCCATCTTTGCTAGAAGAAAACAA AGTCCACCCAGATTTGGTAATAGCCAAAACAGTGTCAAAATCTCCAGAAAGATTAAGAAAAGATGTGGAA GCAATATCAGAAGATACTGATTTTGAAGAGGAAGATGAAATCACAAAAAAGAGAAAGGATGTTAAAAAGG ACACAACAGATAAGGCTTTAAAGCCACAAACCAAAACGTGGGGAAAAGACCGGTATTGTATACAGACGAA TTGTTTGCAGAGTGGATCCCCTGGCAAGAAGGAAGACAGAACCAAGAGCAAAGAGCCACTCTGCACAGGA AACAGTAGTAATAGCTCCTCAGATGAAGACGAAGAGGAAAAGTCCAAAGCAAAGATGACACCAACCAAGA AATATAATGGCTTAGAAGAAAAGAGAAAATCTTTGCGGACAACTAGTTTCTATTCAGGATTTTCAGAAGT AGCAGAAAAAAGGATAAAACTTTTAAATAACTCTGATGAAAGACTCCAGAACAACAGGGCTAAAGATAGA AAAGACGTCTGGTCAAGTATTCAGGGACAGTGGCCTAAGAAAACACTAAAGGAGCTTTTCTCGGACTCTG ACACTGAGGCTGCAGCTTCTCCACCCCATGCTGCCCCAGATGAAGGGACAGTAGAGGAGTCACTGCAGAC TGTGGCAGAAGAGGAGAGTTGCTCACCCAATATGGAGCTAGAGAAGCCACTACCTACCAGTGTTGACAGT AAGCCAGTGGAAGAAAAGCCCCTAGAAGTTAGTGACAGAAAAACAGAATTTCCGAGCAGTGGCAGTAATT CAGTGCTCAATACCCCACCTACCACACCGGAGTCGCCTTCTTCAGTCACTGTAACAGAAACTAGTCAGCA GCAGTCTTCTGTCACGGTGTCAGTGCCCCTGCCTCCAAACCAGGAAGAGGTCCGAAGCATCAAGAGTGAA ACTGACAGCACAATTGAGGTGGACAGTGTTGTGGGGGAGCTCCAGGACCTCCAGTCAGAGGGGAATAGCT CACCAGCAGGCTTTAATGCAAGCGTGAGCTCCAGCAGTAGCAATCAGCCAGAACCAGAACACCCAGAAAA AGCCTGTACAGGTCAGAAAAGAGTGAAAGATACTCAGGGAGTAGGAAGTTCATCAAAAAAGCAGAAGAGA AGCCATAAAGCCACTGTGGTGAACAACAAAAAGAAGGGAAAAGGCACAAACAGTAGTGACAGTGAAGATC TTTCAGCTGGTGAAAGTGTGACTAAGACTCAGGCAATCAAGTCAGTCCCCACTGGAATGAAGACCCACAA TAGCAAGTCTCCTGCAAGAATACAGTCTCCAGGAAAATGTGGCAAGAATGGAGATAAAGATCCTGATCTC AAGGAGCCCAGTAACCGGCTTCCCAAGGTTTACAAGTGGAGTTTTCAGATGTCGGACCTGGAAAATATGA CAAGTGCTGAACGTATCTCAATTCTTCAAGAAAAATTGCAAGAAATCAGAAAACATTATCTCTCATTAAA ATCCGAAGTAGCATCCATTGATAGGAGGAGAAAACGTTTAAAGAAGAAAGAGAGAGAAAGTGCTGCTACA TCTTCCTCCTCCTCCTCTCCCTCATCCAGCTCCATAACAGCTGCTGTTATGTTGACACTAGCTGAGCCGT CAATGTCCAGTGCATCACAGAATGGAATGTCAGTTGAGTGCAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053421
Insert Size	3687 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053421.1, NP_445873.1
RefSeq Size	4980 bp
RefSeq ORF	3687 bp
Locus ID	84481
UniProt ID	Q9JKB5
Gene Summary	Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor.[UniProtKB/Swiss-Prot Function]

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