Tpm1 (NM_001034075) Rat Untagged Clone
CAT#: RN216140
Tpm1 (untagged) - Rat tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.13
CNY 2,380.00
货期*
3周
规格
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Alpha-tm; Tma2; Tmsa |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN216140 representing NM_001034075
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTAGCAGCTCGCTGGAGGCGGTGCGGAGAAAGATCCGGAGCCTGCAGGAACAGGCAGATGCCG CGGAGGAGCGTGCGGGCAGCCTGCAGCGAGAGCTGGACCAGGAGCGGAAGCTGCGGGAAACCGCTGAAGC TGACGTAGCATCTCTGAACAGACGCATCCAGCTGGTTGAGGAGGAGTTGGATCGCGCTCAGGAGCGTCTG GCCACAGCTCTACAGAAGCTGGAGGAGGCTGAGAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCA TTGAAAGCCGAGCCCAAAAAGATGAAGAAAAGATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCA CATTGCTGAAGATGCTGACCGAAAGTATGAAGAGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGATCTG GAGCGTGCGGAGGAGAGGGCTGAGCTCTCGGAAGGCAAATGTGCCGAGCTTGAAGAAGAGTTGAAAACGG TGACGAACAACTTGAAGTCACTGGAGGCTCAGGCTGAGAAGTACTCTCAGAAAGAAGACAAGTATGAAGA GGAGATCAAGGTTCTCTCTGACAAGCTGAAGGAGGCTGAGACCCGGGCTGAGTTTGCAGAGAGATCAGTA ACCAAATTGGAGAAAAGCATTGATGACTTAGAAGACGAGCTGTATGCTCAGAAACTGAAGTACAAGGCCA TCAGCGAGGAGCTGGACCACGCTCTCAACGATATGACTTCCATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001034075 |
Insert Size | 747 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001034075.1, NP_001029247.1 |
RefSeq Size | 1182 bp |
RefSeq ORF | 747 bp |
Locus ID | 24851 |
UniProt ID | P04692 |
Gene Summary | This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (Tpm1.13, also known as variant 9) has multiple differences in the coding region, compared to variant 1. These differences result in an isoform (Tpm1.13) that is shorter and has distinct N- and C- termini compared to isoform a. Isoform Tpm1.13 is the same length as isoforms Tpm1.8cy and Tpm1.9cy but is the product of alternate splicing and contains short regions of sequence differences. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR216140 | Tpm1 (myc-DDK-tagged) - Rat tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.13 |
CNY 3,990.00 |
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