Hfe (NM_001173434) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216212 representing NM_001173434 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGATCAGCTGGGCTCCCTGTGCGGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGTGGTCCGTGG CCCCGCAGGCGCTGCGGCCCGTCACGAAGTTGAGAGTGGTGCCTGAGTCTCACATCCTGCAGGTGATCCT AGGATGTGAGGTGCATGAAGACAACAGTACCAGTGGCTTCTGGAAATATGGCTACGATGGGCAAGATCAC CTTGAATTCTGCCCCAAGACACTGAACTGGAGTGCAGCCGAGCCAAGGGCCTGGGCCACCAAGATGGAGT GGGAAGAGCACAGGATCCGTGCCAGACAGAGCAGGGACTACCTGCAGAGGGACTGCCCCCAGCAGCTGAA GCAGGTCCTGGAGCTCCAGAGAGGGGTTCTGGGACAGCAAGTGCCTACTTTGGTGAAAGTGACTCGCCAC TGGGCCTCTACAGGGACCTCTCTAAGGTGTCAGGCTCTGAATTTCTTCCCCCAGAACATCACTATGAGGT GGTTGAAGGACAGCCAGCCCCTAGATGCCAAGGATGTCAACCCTGAGAACGTGCTGCCAAATGGGGATGG GACCTATCAGGGCTGGCTGACCTTGGCTGTGGCCCCTGGAGAAGAGACAAGGTTCAGCTGTCAAGTGGAG CACCCAGGCCTGGATCAGCCTCTCACTGCCACTTGGGAGCCCTCACGGTCTCAGGACATGATTATTGGAA TCATAAGTGGGATCACCATTTGTGCCATCTTCTTTGTTGGAATTCTGATCCTAGTCTTAAGGAAAAGGAA GGTTTCAGGAGGAACCATGGGTGACTATGTCTTAACAGAGTGTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001173434
Insert Size	819 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001173434.1, NP_001166905.1
RefSeq Size	1406 bp
RefSeq ORF	819 bp
Locus ID	29199
Gene Summary	membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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