Calu (NM_001033898) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cbp50; Rcn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216338 representing NM_001033898 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGCGTCAGTTTCTTATGTGCCTGTCCCTGTGCACGGCCTTTGCTTTGAGCAAGCCTACAGAAA AGAAGGACCGAGTACACCATGAACCTCAGCTCAGCGACAAAGTTCACAACGATGCTCAGAATTTTGACTA TGACCATGATGCCTTCTTGGGAGCAGAAGAGGCAAAGAGTTTTGATCAGCTGACACCAGAAGAGAGCAAG GAAAGGCTTGGAATGATTGTAGATAAAATAGACACCGATAAAGATGGGTTTGTGACGAAGGGGGAGCTGA AGTCCTGGATTAAGCACGCCCAGAAGAAATACATATATGACAATGTTGAAAACCAGTGGCAGGAGTTTGA TATGAATCAAGACGGCTTAATCTCCTGGGATGAGTACAGAAACGTGACTTATGGCACTTACCTGGATGAT CCAGACCCTGATGATGGATTTAATTATAAACAGATGATGGTTAGAGATGAGCGGAGGTTCAAAATGGCAG ACAAAGATGGAGACCTAATTGCCACAAAGGAGGAGTTTACCGCTTTTCTGCACCCTGAGGAATATGACTA CATGAAAGACATAGTCGTGCAGGAAACCATGGAGGATATAGACAAGAATGCTGATGGTTTTATTGATCTA GAAGAGTATATTGGTGACATGTACAGTCATGATGGGAATGCTGATGAACCAGAGTGGGTGAAGACAGAGC GGGAGCAGTTCGTTGAGTTTCGAGATAAGAACCGGGATGGAAAGATGGACAAGGAAGAGACCAAAGACTG GATCCTCCCTTCAGACTATGACCATGCAGAGGCCGAAGCCAGGCATCTCGTCTATGAGTCCGACCAAGAC AAGGATGGCAAGCTCACCAAGGAGGAGATTGTCGACAAGTATGATTTATTTGTGGGCAGCCAGGCCACAG ATTTCGGGGAGGCCTTAGTACGACACGATGAGTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001033898
Insert Size	948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001033898.1, NP_001029070.1
RefSeq Size	3203 bp
RefSeq ORF	948 bp
Locus ID	64366
Gene Summary	binds calcium; localized to the endoplasmic reticulum lumen [RGD, Feb 2006] Transcript Variant: This variant (2) lacks one alternate in-frame exon and contains another alternate in-frame exon compare to variant 1. The resulting isoform (b) is of the same size as isoform a, but it differs in its internal aa sequence.

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