Selenop (NM_001083911) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sepp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216536 representing NM_001083911 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGAAGCCTAGGGCTTGCCCTGGCTCTCTGTCTCCTCCCCTATGGAGGAGCAGAGAGCCAAGGCC AAAGCCCTGCTTGTAAGCAAGCTCCACCCTGGAACATAGGAGATCAAAATCCAATGCTAAACTCCGAGGG CACAGTGACAGTGGTTGCTCTTCTTCAAGCCAGCTGATACCTGTGCCTTCTGCAGGCATCCAGATTGGAA GACCTGCGAATAAAACTAGAGAACCAAGGATATTTTAACATCTCCTATATTGTTGTTAATCATCAAGGAT CTCCTTCCCAATTAAAACATGCACATCTTAAAAAGCAGGTGTCAGATCACATTGCTGTTTACAGACAAGA TGAACATCAAACAGATGTCTGGACACTCTTAAATGGAAACAAAGATGACTTCCTCATATATGACAGATGT GGCCGTCTTGTGTATCACCTTGGTTTGCCCTACTCCTTCCTCACTTTCCCGTATGTTGAAGAAGCCATCA AGATCGCTTACTGTGAGAAGAGGTGTGGAAACTGCTCTTTCACGAGTCTTGAAGATGAAGCCTTCTGTAA AAACGTGTCCTCGGCTACTGCAAGTAAAACCACAGAGCCCTCAGAGGAGCATAACCACCACAAGCACCAT GACAAACATGGGCATGAGCATCTTGGGAGCAGTAAGCCTTCAGAGAATCAGCAACCAGGGGCATTAGATG TTGAGACAAGTCTTCCTCCTTCAGGCTTGCACCACCACCACCACCACCATAAGCACAAGGGCCAGCACAG GCAGGGTCACTTAGAGAGCTGAGACATGGGGGCAAGTGAAGGCTTGCAACTTTCACTTGCCCAGAGGAAG CTCTGACGAAGGGGATGCATAAACCAGCTCCTGTGTAAGTTATCTGAGGAGTCTGGGGCAGCTACCAGTA GCTGCTGCTGCCACTGCCGACACCTCATATTTGAGAAGTCAGGATCTGCAATCACTTGACAGTGTGCCGA AAACCTCCCATCCTTGTGTAGCTGACAGGGGCTTTTCGCGGAGGAGAAAGTCATTGAATCCTGTCAATGT AGATCACCTCCAGCTGCCTGACACAGTCAGCATGTAAGCCCCACAGAAGCCAGCCCCAACTGAAGCTGAA ATAATAAGACCAAGAAGTGAAAATGAAATTTGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001083911
Insert Size	1158 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001083911.1, NP_001077380.1
RefSeq Size	2115 bp
RefSeq ORF	1158 bp
Locus ID	29360
UniProt ID	P25236
Gene Summary	This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in rat), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2017] Transcript Variant: This variant (2, also known as Sepp1b) uses an alternate 5' non-coding exon, hence has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein containing 10 selenocysteine residues.

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