Kiss1r (NM_001301151) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gpr54
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216556 representing NM_001301151 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCAGAGGCGACGTTGGGTCCGAACGTGAGCTGGTGGGCTCCGTCCAACGCTTCGGGATGCCCGG GCTGCGGTGTCAATGCCTCGGATGGCCCAGGCTCCGCGCCAAGGCCCCTGGATGCCTGGCTGGTGCCCCT GTTTTTCGCTGCCCTAATGTTGCTGGGGCTAGTCGGGAACTCACTGGTCATCTTCGTTATCTGCCGCCAC AAGCACATGCAGACCGTCACCAATTTCTACATCGCTAACCTGGCGGCCACAGATGTCACTTTCCTTCTGT GCTGCGTACCCTTCACCGCGCTCCTCTATCCGCTGCCCACCTGGGTGCTGGGAGACTTCATGTGCAAATT CGTCAACTACATCCAGCAGGTCTCGGTGCAAGCCACATGTGCCACTTTGACAGCCATGAGTGTGGACCGC TGGTACGTGACTGTGTTCCCGCTGCGTGCACTTCACCGCCGCACTCCGCGCCTGGCCCTGACTGTCAGCC TTAGCATCTGGGTGGGTTCCGCAGCTGTTTCCGCCCCGGTGCTGGCTCTGCATCGCCTGTCGCCCGGGCC TCACACCTACTGCAGTGAGGCGTTTCCCAGCCGTGCCCTGGAGCGCGCTTTCGCGCTCTACAACCTGCTG GCCCTATACCTGCTGCCGCTGCTCGCCACCTGCGCCTGCTACGGTGCCATGCTGCGCCACCTGGGCCGCG CCGCTGTACGCCCCGCACCCACTGATGGCGCCCTGCAGCTGCTAGCACAGCGCGCTGGAGCAGTGCGCAC CAAGGTCTCCCGGCTGGTGGCCGCTGTCGTCCTGCTCTTCGCCGCCTGCTGGGGCCCGATCCAGCTGTTC CTGGTGCTTCAAGCCCTGGGCCCCTCGGGGGCCTGGCACCCTCGAAGCTATGCCGCCTACGCGCTCAAGA TCTGGGCTCACTGCATGTCCTACAGCAATTCTGCGCTCAACCCGCTGCTCTATGCCTTCCTGGGTTCCCA CTTCAGACAGGCCTTCTGCCGCGTGTGCCCCTGCGGCCCGCAACGCCAGCGTCGGCCCCACGCGTCAGCG CACTCGGACCGAGCCGCACCCCATAGTGTGCCGCACAGCCGGGCTGCGCACCCTGTCCGGGTCAGAACCC CCGAGCCTGGGAACCCTGTGGTGCGCTCGCCCTCTGTTCAGGATGAACACACTGCCCCACTCTGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001301151
Insert Size	1185 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301151.1, NP_001288080.1
RefSeq Size	1496 bp
RefSeq ORF	1185 bp
Locus ID	78976
Gene Summary	a G protein-coupled receptor; human homolog may bind ligand and metastasis suppressor gene KiSS-1 [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate, in-frame acceptor splice site at the 3' terminal exon compared to variant 1. The resulting shorter isoform (2) lacks two internal amino acids compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.