Sgk1 (NM_001193569) Rat Untagged Clone

CAT#: RN216618

Sgk1 (untagged) - Rat serum/glucocorticoid regulated kinase 1 (Sgk1), transcript variant 2



  "NM_001193569" in other vectors (1)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Sgk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN216618 representing NM_001193569
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAGGAGGCCTTAAGATCCCCTTGGAAAGCTTTCATGAAACAGAGAAGGATGGGCCTGAACGATT
TTATTCAGAAGCTTGCCAACAACTCCTATGCATGCAAACACCCTGAAGTTCAATCCTATTTGAAAATCTC
CCAACCTCAGGAGCCCGAACTTATGAACGCCAACCCCTCACCTCCTCCAAGTCCCTCTCAACAAATCAAC
CTGGGTCCATCCTCAAATCCCCACGCCAAACCCTCTGACTTCCACTTCTTGAAAGTGATCGGAAAAGGCA
GTTTTGGAAAGGTTCTTCTAGCAAGGCACAAGGCAGAAGAAGCATTCTATGCCGTCAAAGTTTTGCAGAA
GAAAGCCATCTTGAAGAAGAAGGAGGAGAAGCATATTATGTCAGAGCGCAATGTTCTGTTGAAGAATGTG
AAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAACTCTACTTCGTCCTAGACT
ACATTAATGGCGGAGAGCTGTTCTACCATCTCCAGAGGGAGCGCTGCTTCCTGGAACCCCGTGCTCGCTT
CTACGCAGCTGAAATAGCCAGTGCCTTGGGTTATCTGCACTCCCTAAACATCGTTTATCGAGACTTAAAA
CCAGAGAATATTCTCCTAGACTCACAGGGACACATCGTCCTCACTGACTTTGGGCTCTGCAAGGAGAACA
TCGAGCACAATGGGACAACGTCCACCTTCTGTGGCACGCCTGAGTATCTCGCTCCTGAGGTTCTCCATAA
GCAGCCGTACGACCGGACAGTGGACTGGTGGTGCCTCGGGGCTGTCTTGTATGAGATGCTCTATGGCCTG
CCTCCGTTCTACAGCCGGAACACAGCCGAGATGTATGACAATATTCTGAACAAGCCTCTCCAGCTGAAAC
CAAATATCACCAACTCAGCAAGGCACCTGCTGGAGGGCCTCCTGCAGAAGGACCGGACCAAGAGGCTGGG
TGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTTCTTCTCTTTGATTAACTGGGATGATCTCATT
AATAAGAAGATCACGCCCCCATTTAACCCAAATGTGAGCGGGCCCAGTGACCTTCGGCACTTTGATCCCG
AGTTTACTGAGGAGCCGGTCCCCAGCTCCATCGGGCGATCCCCTGACAGCATCCTTGTCACAGCCAGTGT
GAAAGAAGCCGCGGAAGCCTTCCTTGGCTTCTCCTATGCCCCTCCTATGGACTCCTTCCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001193569
Insert Size 1254 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001193569.1, NP_001180498.1
RefSeq Size 2493 bp
RefSeq ORF 1254 bp
Locus ID 29517
Gene Summary may play a role in menory consolidation and spatial learning; may mediate aldosterone-induced signaling [RGD, Feb 2006]
Transcript Variant: This variant (2) represents use of an alternate promoter, 5' UTR, and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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