Smyd2 (NM_206851) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	HSKM-B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216661 representing NM_206851 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGCCGAGGCCCGCGGCGGCCTGGAGCGCTTCTGCAGCGCGGGCAAGGGCCGGGGGCTCCGTGCGC TGCGGCCCTTCCACGTGGGCGACCTCCTCTTCTCCTGCCCGGCCTACGCCTGCGTGCTCACCGTGGGCGA GCGGGGCCACCACTGCGAGTGCTGCTTCGCCAGGAAAGAAGGATTGTCGAAATGTGGACGATGCAAGCAA GCCTTCTACTGCGATGTGGAGTGTCAGAAAGAAGACTGGCCCCTGCACAAGCTGGAGTGCTCCTCCATGG TTGTTTTCGGGGAGAACTGGAATCCCTCGGAGACTGTGCGGCTCACAGCAAGGATCCTGGCCAAGCAGAA AATGCACCCAGAGAGGACACCTTCAGAGAAACTGTTGGCCGTGAGGGAGTTTGAGTCACATCTGGACAAG CTAGACAACGAGAAGAAGGATCTCATCCAGAGCGACATCGCGGCGCTCCATCAGTTCTACTCCAAGCACC TGGAGTTCCCTGACCACAGCAGCCTTGTGGTGCTCTTTGCCCAGGTGAACTGTAATGGCTTCACTATTGA AGATGAGGAGCTCTCTCACTTGGGATCGGCGATATTTCCTGATGTTGCGCTGATGAATCACAGCTGCTGC CCGAATGTCATTGTGACCTACAAAGGTACCCTGGCAGAAGTCAGAGCTGTGCAGGAGATCCACCCAGGAG ATGAGGTGTTCACCAGCTATATCGACCTGCTGTATCCAACAGAAGACAGGAACGACCGGTTAAGAGACTC CTACTTCTTTACCTGTGAGTGCCGGGAGTGTACGACCAAGGACAAGGACAAGGCCAAGGTGGAAATCCGG AAGCTCAGCAACCCACCTCAGGCAGAAGCCATCCGAGACATGGTCAGATACGCACGCAATGTCATCGAGG AGTTCCGGAGGGCCAAGCACTACAAATCCCCTAGTGAGCTGTTGGAAATCTGTGAGCTCAGCCAGGAGAA GATGAGCTCTGTGTTTGAGGACAGCAATGTGTACATGCTACACATGATGTACCAGGCCATGGGCGTCTGC CTGTACATGCAGGACTGGGAAGGAGCCCTGAAATATGGCCAGAAGATCATCAAACCCTACAGTAAGCACT ACCCCGTGTACTCCCTCAACGTGGCCTCCATGTGGCTGAAGTTGGGAAGACTGTACATGGGCCTGGAGAA CAAAGCTGCCGGGGAGAAGGCCCTGAAGAAGGCCATCGCCATCATGGAAATAGCTCATGGCAAGGACCAC CCGTACATCTCCGAGATCAAGCAGGAAATTGAGAGCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_206851
Insert Size	1302 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_206851.1, NP_996733.1
RefSeq Size	1592 bp
RefSeq ORF	1302 bp
Locus ID	289372
UniProt ID	Q7M6Z3
Gene Summary	Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860' (By similarity).[UniProtKB/Swiss-Prot Function]

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