Eno1 (NM_012554) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nne
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216663 representing NM_012554 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATTCTCAAGATCCATGCCAGAGAGATCTTTGACTCCCGCGGGAATCCCACCGTTGAGGTGGATC TCTACACCGCAAAAGGTCTCTTCCGTGCTGCGGTGCCCAGCGGTGCGTCCACTGGCATCTACGAGGCCCT AGAACTCCGAGACAATGATAAGACCCGCTTCATGGGGAAGGGTGTCTCAAAGGCTGTTGAGCACATCAAT AAAACTATTGCACCTGCTCTGGTTAGCAAGAAACTGAATGTTGTGGAGCAGGAGAAGATTGACCAGCTGA TGATCGAGATGGACGGCACAGAGAATAAATCTAAGTTTGGTGCAAATGCCATCCTGGGAGTGTCCCTGGC TGTCTGCAAGGCTGGTGCCGTGGAGAAGGGGGTGCCCCTTTACCGTCACATTGCCGACTTGGCCGGCAAC CCTGAAGTCATCCTGCCGGTCCCAGCTTTCAATGTGATCAACGGCGGTTCTCATGCTGGCAACAAGTTGG CCATGCAAGAGTTCATGATCCTGCCTGTGGGGGCATCCTCTTTCCGGGAAGCCATGCGCATTGGAGCAGA GGTTTACCACAACCTGAAGAACGTCATCAAGGAGAAGTACGGGAAAGACGCCACCAATGTGGGTGATGAG GGTGGATTCGCACCTAACATCCTGGAGAACAAAGAAGCACTGGAGCTGCTCAAGTCTGCCATTGCAAAGG CCGGCTACACTGACCAGGTTGTCATCGGCATGGATGTGGCTGCCTCCGAGTTCTACAGGGCTGGCAAGTA TGACCTGGACTTCAAGTCTCCAGATGATGCCAGCCGGTACATCACACCCGACCAGCTGGCCGACCTGTAC AAGTCCTTCATCAAGGACTACCCAGTGGTGTCCATTGAAGATCCCTTTGACCAGGACGACTGGGATGCTT GGCAGAAGTTCACAGCTACTGCAGGCATCCAGGTGGTGGGGGATGACCTCACAGTGACCAACCCTAAGCG GATCGCCAAGGCTGCAGGCGAAAAGTCCTGCAACTGCCTCCTGCTCAAAGTGAACCAGATTGGCTCTGTG ACCGAGTCTCTGCAGGCGTGTAAGCTGGCCCAGTCCAATGGCTGGGGTGTCATGGTGTCCCATCGATCTG GGGAGACTGAGGACACTTTCATTGCCGACCTGGTGGTGGGGCTCTGCACTGGGCAGATCAAGACTGGTGC CCCCTGCCGATCTGAGCGCCTGGCCAAGTACAATCAGATCCTTAGAATCGAGGAGGAGCTGGGCAGCAAA GCCAAGTTTGCCGGCAGGTCCTTCAGGAACCCCCTGGCCAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012554
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012554.3, NP_036686.2
RefSeq Size	1754 bp
RefSeq ORF	1305 bp
Locus ID	24333
UniProt ID	P04764
Gene Summary	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein.

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