Hnf4a (NM_001270933) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hnf4; Hnf4alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216675 representing NM_001270933 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACAGGGGCCAGGGTTTCCTGCAGGACCCACCAGACACATCCCCATCTGAAGGTGCCAACCTCA ACTCATCCAACAGCCTGGGTGTCAGTGCCCTGTGTGCCATCTGTGGCGATCGGGCCACTGGCAAACACTA CGGAGCCTCAAGCTGTGACGGCTGCAAGGGATTCTTCAGGAGGAGCGTGAGGAAGAACCACATGTACTCC TGCAGGTTTAGCAGGCAGTGCGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCTCAAGA AGTGCTTCCGGGCTGGCATGAAGAAAGAAGCCGTCCAAAATGAGCGGGATCGGATCAGCACGCGGAGGTC AAGCTACGAGGACAGCAGCCTACCCTCCATTAATGCGCTCCTGCAGGCAGAGGTCCTGTCTCAGCAGATC ACCTCCCCCATCTCTGGGATCAATGGCGACATTCGGGCCAAGAGGATTGCCAACATCACGGATGTGTGTG AGTCTATGAAGGAGCAGCTGCTGGTTCTGGTCGAATGGGCCAAGTACATCCCGGCCTTCTGTGAACTTCT TCTGGATGACCAGGTGGCGCTGCTCAGAGCCCACGCTGGTGAGCACCTGCTGCTTGGAGCCACCAAGAGG TCCATGGTGTTCAAGGATGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGAGCTAG CAGAGATGAGCCGTGTGTCCATTCGCATCCTCGATGAGCTGGTCTTGCCCTTCCAAGAGCTGCAGATCGA TGATAATGAATACGCCTGCCTCAAAGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGTGACCCA GGCAAGATCAAGCGGCTGCGGTCACAGGTGCAGGTGAGCCTGGAGGATTACATCAACGACCGGCAGTATG ACTCTCGGGGTCGTTTTGGAGAGCTGCTGCTGCTCCTGCCCACTCTGCAGAGCATTACCTGGCAGATGAT CGAGCAGATCCAGTTCATCAAGCTCTTTGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGCTGCTT GGAGGGTCTGCCAGTGACGCGCCCCACGCCCACCACCCCCTGCACCCTCACCTGATGCAAGAACACATGG GCACCAATGTCATAGTTGCCAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTCCACCCCTGAGAC TCCACAGCCATCACCACCAAGTGGCTCTGGATCTGAATCCTACAAGCTCCTGCCAGGAGCCATCACCACC ATCGTCAAGCCTCCCTCTGCCATCCCCCAGCCAACGATCACCAAGCAGGAAGCCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270933
Insert Size	1320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270933.1, NP_001257862.1
RefSeq Size	4426 bp
RefSeq ORF	1320 bp
Locus ID	25735
Gene Summary	steriod horomone receptor; involved in promoting transcription [RGD, Feb 2006] Transcript Variant: This variant (3) differs in the 5' UTR and has two coding region differences, compared to variant 1. The resulting isoform (3, also known as HNF4alpha10) is shorter, has a distinct N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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