Azin1 (NM_001301702) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Oazi; Oazin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216695 representing NM_001301702 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGATTTATTGACGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG TTATTGATAACTATGTTTATGAACATACCCTGACAGGAAAAAATGCATTTTTTGTGGGGGATCTTGGGAA GATCGTGAAGAAGCACAGTCAGTGGCAGAACGTGGTGGCTCAGATAAAGCCATTTTACATGGTGAAGTGC AACTCCACTCCAGCCGTACTGGAGATCTTGGCAGCTCTTGGAACTGGATTTGCTTGTTCCAGCAAAAATG AAATGGCTTTAGTGCAAGAATTAGGCGTATCTCCAGAAAACATCATTTATACAAGTCCTTGTAAGCAAGC ATCTCAGATAAAGTACGCAGCAAAAGTTGGAGTAAATATTATGACATGTGACAATGAAGTTGAATTAAAG AAAATTGCGAGGAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG AAGATGGTAATATGAAGTTTGGCACTACACTGAAGAACTGTAGGCATCTTTTGGAATGTGCCAAGGAACT TGATGTCCAAATAATTGGGGTTAAATTTCATATTTCAAGTGCTTGCAAAGAATATCAAGTCTATGTCCAT GCCCTGTCTGATGCTCGATGTGTGTTTGACATGGCTGGGGAGTTTGGCTTTACAATGAACATGTTAGACA TCGGTGGAGGCTTCACAGGAACTGAAATTCAGTTGGAAGAGGTTAATCATGTTATCAGTCCTCTGTTGGA TATTTACTTCCCTGAAGGATCTGGCATTCAGATCATTTCCGAACCTGGAAGCTACTATGTATCTTCTGCA TTTACGCTTGCAGTCAATATTATTGCTAAGAAAGTTGTTGAAAATGATAAATTATCCTCTGGAGTAGAAA AAAATGGGAGTGACGAGCCAGCCTTCGTGTACTACATGAATGACGGTGTTTATGGTTCTTTTGCAAGTAA GCTTTCTGAGGACTTAAATACTGTTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA AGCAGCCTTTGGGGTCCATCCTGTGACGAGCTTGATCAAATTGTGGAAAGTTGTCTTCTTCCTGAGCTGA GCGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTTGCACGGACCGTCTGCTTTTAGTGA CACTCAGAGGCCAGCTATTTATTTCATGATGTCACTCAGTGATTGGTATGAGATGCAAGATGCTGGAATT ACTTCAGATGCAATGATGAAAAACTTCTTCTTTGCACCCTCTTGCATTCAGCTGAGCCAAGAAGACAACT TCTCCACTGAAGCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301702
Insert Size	1347 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301702.1, NP_001288631.1
RefSeq Size	4186 bp
RefSeq ORF	1347 bp
Locus ID	58961
Gene Summary	The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (2) lacks an internal 5' non-coding exon, thus has a shorter 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.