Mapk10 (NM_001270556) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Jnk3; SAPb; SAPKC; Serk2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216728 representing NM_001270556 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACCTTGGATGTGAAAATTGCCTTTTGTCAGGGAT TCGACAAACACGTGGATGTGTCTTCTGTTGCCAAACATTACAACATGAGCAAAAGCAAGGTAGATAACCA GTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTAAAGCGCTACCAGAACCTGAAGCCGATC GGCTCTGGGGCTCAGGGAATAGTTTGTGCTGCGTATGACGCTGTCCTCGACAGAAATGTGGCCATTAAGA AGCTCAGCAGACCCTTCCAGAACCAAACTCATGCCAAGAGGGCTTACCGGGAGCTGGTCCTCATGAAGTG TGTGAACCATAAAAACATTATTAGCTTATTAAATGTCTTTACACCCCAGAAAACACTGGAGGAGTTCCAA GATGTTTACTTAGTGATGGAACTGATGGACGCCAACTTGTGTCAGGTGATTCAGATGGAGCTGGACCACG AGCGGATGTCGTACTTGCTGTACCAGATGCTGTGCGGCATCAAACACCTCCACTCCGCTGGGATCATCCA CAGGGACTTAAAACCCAGTAACATCGTAGTCAAGTCTGATTGCACACTGAAAATCCTGGACTTTGGACTG GCCAGGACAGCGGGCACAAGCTTCATGATGACTCCGTATGTGGTGACGAGATATTACAGAGCCCCCGAGG TCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCTGTGGGCTGCATCATGGGAGAAATGGT TCGTCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGTGGAACAAAGTCATAGAGCAGCTAGGA ACTCCGTGTCCAGAATTCATGAAGAAATTGCAGCCCACCGTCAGAAACTACGTGGAGAACCGGCCCAAGT ATGCAGGCCTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCCGAGCACAATAAACT TAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTG GATGACGCATTGCAGCATCCGTACATCAACGTTTGGTACGACCCTGCTGAAGTGGAGGCGCCTCCGCCTC AGATATATGACAAGCAACTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGAACTCATCTACAAGGA AGTAATGAACTCAGAAGAGAAGACTAAGAACGGCGTAGTCAAAGGCCAGCCCTCACCTTCAGGTGCAGCA GTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAACGACATCTCCTCCATGTCCACCGACCAGA CCCTCGCATCCGACACTGACAGCAGCCTGGAAGCCTCGGCGGGACCGCTGGGTTGTTGCAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270556
Insert Size	1395 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270556.1, NP_001257485.1
RefSeq Size	2759 bp
RefSeq ORF	1395 bp
Locus ID	25272
Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017] Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1).

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