Slc19a1 (NM_001035232) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MTX1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216818 representing NM_001035232 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCCACTGGCCAGGTGGCAGAAAAGCAAGCCTGCGAAGAGCCCAGGCAGGACCGAGAACTTAAGT CCTGGCGATGGCTCGTGTTCTACCTCTGCTTCTTTGGCTTCATGGCACAGTTGCGACCTGGGGAGAGCTT CATCACACCCTACCTCCTGGAACGTAACTTCACCAAGGAGCAGGTGACCAACGAGATCATTCCGATGCTG CCCTACTCCCACCTGGCCGTGCTGGTCCCCATCTTCCTGCTCACCGACTACCTACGATACAAGCCAGTCC TGGTCCTACAGTGCCTGAGCTTCGTGTGCGTGTGGCTGCTGCTGCTCCTGGGGACCTCCGTCGTGCACAT GCAGCTCATGGAAGTCTTCTACAGCATCACCATGGCGGCGCGCATCGCCTACTCCTCCTACATATTCTCC CTGGTGCAGCCCTCCCGCTACCAGCGCATGGCCAGCTACTCGCGGGCCGCAGTCCTACTGGGCGTCTTCA TCAGCTCCGTGCTGGGCCAGGTGCTGGTCACCCTAGGAGGCATCTCCACCTATATGCTCAACTGTATCTC CCTGGGCTTCATCCTCTTCAGCCTGAGCCTCTCCCTCTTCCTAAAGCGTCCTAAGCGGAGCCTCTTTTTC AACCGGAGCGCACTTGTGCAAGGAGCCTTGCCCTGTGAGCTGGATCAGATGCACCCGGGACCTGGCCGTC CAGAGCCCAGGAAACTGGAGCGCATGCTGGGCACTTGCAGGGACTCCTTCCTGGTACGCATGCTAAGCGA ACTGGTGAAGAACGTCCGGCAACCACAGCTGCGTCTCTGGTGCCTCTGGTGGGTCTTCAACTCGGCAGGC TACTACCTGATCACCTACTATGTCCATGTCCTGTGGAAAATCACTGACAGCAGACTCAACTACAACGGTG CCGTAGATGCCGCCTCCACACTTCTGAGCGCCATCACGGCCTTCACCGCGGGCTTCGTGAACATCCGCTG GGCTCTGTGGTCAAAGCTGGTCATCGCGAGTGTCATAGCCATCCAGGCTGGCCTGGTTTTCTGTATGTTC CAGATCCCGGACATCTGGGTCTGCTATGTGACCTTCGTGCTTTTCCGTGGGGCCTACCAGTTCCTTGTGC CCATTGCCACTTTTCAGATTGCATCTTCCCTGTCTAAAGAGCTCTGTGCATTGGTCTTCGGGATCAACAC TTTCCTTGCCACTGCGCTTAAGACCTCGATCACACTCGTGGTCTCTGACAAGCGGGGTCTGGGCCTCCAA GTCCATCAACAGTTCCGCATCTACTTCATGTACTTCCTGACGCTGTCCATCATCTGCCTTGCCTGGGCTG GGCTGGACGGCTTGAGGTACTACCGGCGTGGTCGCCACCAGCCGCTGGCCCAGGCCCAGGCTCTGAGTCC CTTGGAGGATTCTGTGCAGGCGATAAGCCTACAGGATGGAGACCTAAGGAGACCGCAGCCCTCGGCACCA CAGCTGCTCCCAGAGGATGGCAGCGTAGAAGACGGCAGGGCAGACTTGAGAGTCGAAGCCAAGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001035232
Insert Size	1539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001035232.1, NP_001030309.1
RefSeq Size	2303 bp
RefSeq ORF	1539 bp
Locus ID	29723
UniProt ID	Q62866
Gene Summary	mouse homolog facilitates transport of methotrexate and folic acid [RGD, Feb 2006] Transcript Variant: This variant (2) uses a different segment for its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

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