Shkbp1 (NM_001271080) Rat Untagged Clone

CAT#: RN217125

Shkbp1 (untagged) - Rat Sh3kbp1 binding protein 1 (Shkbp1)



  "NM_001271080" in other vectors (1)

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1309281; Sb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN217125 representing NM_001271080
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCCCGACGACTGCAGTGGAGGGGGTCCGGAGTCGGGGAGCGCCCGGGGAAGTGATTCATCTGA
ACGTAGGGGGCAAGCGGTTCAGCACCTCTCGCCAGACGCTAACCTGGATCCCAGACTCCTTCTTTTCCAG
TCTTTTGAGCGGACGTATCTCAACCCTGAAAGATGAGACCGGAGCCATCTTCATCGACCGGGACCCCACT
GTCTTTGCCCCCATCCTCAATTTCCTGAGGACCAAAGAGTTAGACCCCAGGGGTGTCCATGGTTCCAGCC
TCCTCCATGAAGCCCAATTCTATGGGCTCACTCCACTGGTTCGTCGCCTGCAGGTAAGGGAAGAGTTGGA
TCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGCGGAGA
AACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAACACGA
TGCCCCCCAACTTGGGTAATGCAGGGCTGCTAGGCCGAATGCTGGATGATAGAGCACCTCCATCCCCATC
AGGCCAACCAGAGGAACCAGGGATGGTGCGGCTGGTGTGTGGTCACCACAACTGGATTGCTGTGGCGTAT
ACCCACTTTCTCGTCTGTTATAGACTGAAGGAAGCCTCCGGTTGGCAGCTTGCATTCTCCAGTCCTCGCC
TAGACTGGCCCATTGAGCGCCTGGCTCTCACAGCTCGGGTGCTCGGTGGTGCTCCAGGCGAGCATGACAA
GATGGTGGCTGCAGCCACAGGCAGCGAGATCCTTCTGTGGGCGCTACAGGCACAAGGTGGTGGCTCTGAG
ATAGGTGTCTTCCACCTGGGTGTGCCTGTGGAAGCCTTGTTCTTTGTTGGGAACCAGCTCATTGCCACAA
GCCACACAGGGCGCATCGGTGTCTGGAACGCCGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAGCCTAT
CACGAGTTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATGTAGAT
GTGCAGAAATTCCCCCTGCGCATGAAGGACAACGACCTCCTTGTCAGTGAGCTCTACCGAGACCCAGCAG
AAGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATTGAGAT
TGCCTATGGCACCAGCTCCGGAGTGGTGCGGGTCATTGTCCAGCACCCCGAGACAGTGGGCTCGGGACCC
CAGCTCTTCCAGACCTTCAGTGTACACCGCAGCCCTGTCACCAAGATCATGCTGTCAGAGAAGCACCTCA
TCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATCTCCAC
CCAGCCTGGTTCCACTCCACTGGCATCCTTCAAGATCCTGGCACTTGAATCAGCCGATGGGCTCGGTGGC
TGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAGCGGGATGACCAGCAGGTGTTCATCCAGAAGGTGG
TACCAAATGCCAGCCAGCTGTTTGTGCGTCTCTCCTCCACTGGGCAGCGGGTGTGTTCTGTACGCTCAGT
GGATGGCTCCGCTACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGTTGGGCTCCAGACCC
CGACGCTACCTGCTCACCGGCCAAGCCAATGGCAGCCTGGCCATGTGGGACCTGACCACTGCCATGGACG
GCCTGGGCCAAACCCCTGCAGGCGGCCTGACCGAAGAAGAGCTGATGGACCAACTGGAGCAGTGTGAACT
GAGCCCATTGACTTCTTCTCGGGCCTCTTTTCCCAGCCCCTCACCCAGAACCTCTCTTACCAGTCTACAC
TCAGCCTCCAGCAACACCTCGCTGTGTGGCCACAGAGGGAGCCCCAGCCCACCGCAGGCAGGGGCCCGGA
GCCGCGGGGCAGGAAGCTTCGTGGATCGGTTTAAAGAACTGGCAAGGGGTGCACCGGAGCTCCGAGGGCC
ACCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGATACTTCCCAAAAACACACTTAAC
GAAACTTCCTTTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001271080
Insert Size 2115 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271080.1, NP_001258009.1
RefSeq Size 2300 bp
RefSeq ORF 2115 bp
Locus ID 292735
UniProt ID P0C5J9
Gene Summary Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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