Gars (NM_001271139) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GlyRS; RGD1559871
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217147 representing NM_001271139 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGTCTGCTCCCCACGCTGCTCAGAGCCACCCGCGCCGCGCTGCTCCTACAGTCGCCCCGAGTGG TCGCAGCGCCCGCGTCTCAGCGGCTCCTCAGCGCTCCCGCGCAGCCCGCCGCCTCTCCGAGCAGCATGGA CAGCGCCGAGGAGCTGCTAGCCCCATTGCGGCTAGCTGTGCGCCAGCAGGGAGACTTTGTACGGAAGTTA AAAGAAGATAAAGCCCCACAAGTGGATGTGGACAGAGCAGTAGCTGAGCTCAAGGCCCGGAAGAGGGTTC TGGAAGCAAAGGAGCTGGCGTTGCAGCCCAAAGATGACATCGTAGATAGAGCAAAAATGGAGGATACTTT GAAGAGGAGATTTTTCTACGATCAAGCTTTTGCTATTTATGGAGGTGTCAGTGGTTTGTATGACTTTGGG CCAGTGGGATGTGCTTTGAAGAACAATATCATCCAGACCTGGAGGCAGCACTTTATCCAAGAGGAACAGA TCCTGGAAATTGACTGCACCATGCTCACCCCCGAGCCAGTTTTAAAGACCTCTGGCCATGTAGACAAATT TGCTGACTTCATGGTGAAGGACGTGAAGAACGGAGAGTGCTTCCGCGCGGACCACCTGTTGAAAGCTCAT TTACAGAAACTGATGTCAGATAAGAAGTGCTCTGCTGAGAAGAAATCAGAGATGGAAAGTGTCTTGGCCC AGCTTGATAACTATGGACAACAAGAACTGGCGGATCTTTTTGTGAACTATAATGTAAAATCTCCCACCAC TGGCAATGACCTGTCCCCTCCGGTACCTTTTAACTTAATGTTCCAGACCTTCATTGGGCCTGGAGGAAAC ATGCCTGGATATCTGAGACCAGAAACTGCACAGGGAATTTTCCTAAATTTCAAACGACTTTTGGAATTCA ACCAAGGGAAGTTGCCTTTTGCTGCTGCACAGATTGGAAACTCCTTTAGAAATGAGATCTCGCCCCGGTC TGGACTGATCCGAGTCAGGGAATTCACAATGGCAGAGATCGAGCACTTTGTAGATCCCACTGAGAAAGAC CATCCCAAGTTCCCGAGCGTGGCCGACCTCTACCTTTATTTGTACTCTGCCAAAGCCCAGGTCACTGGAC AGTCTGCTCGCAAGATGCGTCTGGGAGATGCTGTTGAACAGGGTGTGATTAACAACTCAGTATTAGGCTA TTTTATTGGCCGCATCTACCTCTACCTCACGAAGGTTGGAATATCTCCTGATAAACTCCGCTTCCGACAG CATATGGAGAATGAGATGGCCCATTATGCCTGCGACTGCTGGGATGCTGAGTCCAAAACGTCCTATGGCT GGATCGAGATTGTTGGATGTGCTGACCGATCCTGCTATGACCTCTCCTGTCATGCTCGAGCCACCAAAGT CCCGCTGGTGGCTGAGAAGCCCCTGAAAGAGCCCAAAACAGTTAACGTTGTACAGTTTGAGCCCAACAAG GGAGCCGTGGGCAAGGCGTACAAGAAAGATGCAAAGCTGGTGCTGGAGTACCTCGGCGCCTGCGATGAGT GCTACATTACCGAGATGGAGCTGCTGCTGAGCGAGAAAGGGGAATTCACTATTGAAACTGAAGGAAAAAC ATTTCAGTTAACAAAAGACATGGTCAGTGTGAAGAGATTCCAGAAAACACTACACGTGGAAGAAGTTGTT CCGAGTGTAATTGAGCCCTCTTTTGGCCTGGGCAGGATCATGTATACCATATTGGAACATACATTCCACG TCCGGGAGGGAGATGAGCAGAGAACGTTCTTCAGTTTCCCTGCTGTGGTTGCTCCATTCAAATGTTCTGT CCTTCCACTGAGCCAAAACCAAGAGTTTATGCCATTTGTCAAGGAATTATCGGAAGCTCTGACTCGAAAT GGCGTGTCTCATAAAGTTGACGATTCCTCTGGGTCTATTGGAAGGCGCTATGCAAGAACTGATGAGATTG GCGTGGCGTTTGGCATCACCATTGACTTTGATACAGTGAACAAGACCCCCCACACTGCAACTCTGAGGGA CCGAGACTCCATGAGACAGATAAGGGCAGAGGTCTCTGAACTGCCCAGTGTGGTCCGTGATCTGGCCAAT GGCAACATCACTTGGGCTGATGTGGAGGCCAGGTACCCCCTCTTTGAAGGGCAAGAGACTGGCAAGAAGG AGACAGTGGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271139
Insert Size	2187 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271139.2, NP_001258068.1
RefSeq Size	2459 bp
RefSeq ORF	2187 bp
Locus ID	297113
UniProt ID	Q5I0G4
Gene Summary	Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.[UniProtKB/Swiss-Prot Function]

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