Rps6ka2 (NM_057128) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217149 representing NM_057128 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGAACATGAAGAAGTTCACGGTGCGCAGGTTCTTCTCAGTGTACCTGCGCAAGAAGTCGCGCT CCAAGAGCTCCAGTCTGAGTCGCCTCGAGGAAGAAGGCATCGTGAAGGAGATTGACATTAGCAGCCATGT GAAGGAAGGCTTCGAGAAGGCAGACCCCTCCCAGTTCGAGCTGCTAAAAGTTTTAGGACAAGGGTCGTAT GGAAAGGTGTTCTTGGTGAGGAAAGTCACAGGATCGGATGCTGGTCAGCTCTACGCCATGAAAGTCCTGA AGAAAGCCACCTTGAAAGTGCGGGACCGGGTCCGGTCTAAGATGGAGAGAGATATCCTGGCAGAGGTGAA TCACCCTTTCATCGTCAAGCTGCATTACGCCTTTCAGACCGAAGGCAAGCTCTACCTGATCCTGGACTTC CTGCGGGGAGGAGACCTCTTCACCAGGCTTTCCAAAGAGGTGATGTTTACGGAGGAGGATGTTAAGTTCT ACCTGGCTGAGCTGGCCTTGGCTCTGGACCACCTCCACGGCCTAGGGATCATCTACAGGGATCTGAAGCC TGAAAATATCCTCCTGGATGAAGAGGGACATATTAAGATCACAGATTTTGGCTTGAGCAAGGAGGCCATC GACCATGACAAGAGAGCCTATTCATTTTGTGGGACTATTGAATACATGGCGCCCGAGGTGGTGAACCGGC GTGGACACACACAGAGTGCTGACTGGTGGTCCTTTGGTGTGCTCATGTTCGAGATGCTCACAGGATCCCT GCCGTTTCAGGGGAAGGACAGGAAGGAAACAATGGCCCTCATCCTCAAAGCCAAGCTGGGCATGCCTCAG TTCCTCAGTGCGGAGGCACAGAGCCTGCTGAGAGCCCTTTTCAAGCGGAATCCCTGTAACCGGCTAGGTG CTGGTGTTGACGGAGTGGAGGAAATTAAACGTCACCCGTTCTTTGTCACCATAGACTGGAATAAGCTGTA TCGCAAGGAAATCAAGCCACCCTTCAAGCCAGCAGTGGGAAGGCCTGAGGACACCTTCCACTTTGACCCC GAGTTTACTGCAAGGACCCCCACAGACTCTCCTGGTGTCCCCCCAAGTGCAAACGCCCACCACCTGTTCA GGGGGTTCAGCTTTGTGGCCTCCAGCCTGGTCCAGGAGCCTTCACAGCAAGACGTGCCCAAGGCCCCCAT TCACCCGATTGTGCAGCAGTTACATGGGAACAATATCCACTTCACTGATGGCTACGAGATCAAGGAGGAC ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGGTGTGTACACAAAGCCACGGATGCCGAGTACGCCGTGA AGATCATCGATAAGAGCAAAAGGGATCCCTCAGAGGAGATTGAGATCCTCCTGCGGTATGGCCAACACCC CAACATCATCACCCTAAAAGATGTCTATGATGATGGAAAATACGTATACCTGGTGATGGAGCTCATGCGA GGTGGGGAGCTGCTGGACCGCATCCTCCGCCAGCGGTGCTTCTCAGAGCGGGAGGCCAGTGATGTGCTGT ACACCATCGCCAGGACCATGGACTACCTGCACTCCCAAGGGGTTGTTCATCGGGACCTGAAACCAAGTAA CATTCTGTACATGGATGAATCTGGAAACCCCGAATCTATCCGCATCTGTGACTTTGGGTTTGCCAAACAG CTGCGAGCAGAAAATGGACTGCTCATGACCCCCTGCTATACTGCGAACTTCGTGGCTCCTGAGGTCCTGA AGCGGCAAGGCTACGACGCAGCGTGTGATGTCTGGAGCCTGGGAATCCTGCTGTACACCATGCTGGCTGG GTTCACCCCTTTTGCTAATGGGCCAGACGATACCCCCGAGGAGATTCTGGCGAGGATTGGCAGTGGGAAG TACGCCCTTTCTGGAGGAAACTGGGACTCCATATCGGATGCAGCAAAGGACGTCGTGTCCAAGATGCTCC ACGTGGACCCGCAGCAACGCCTAACAGCAGTTCAAGTGTTGAAGCACCCATGGATCGTGAACAGAGAGTA CCTATCCCAAAACCAGCTGAGCAGACAGGACGTCCATCTAGTGAAGGGTGCCATGGCAGCCACCTACTTT GCTCTGAACAGGACCCCACAGGCGCCGAGGCTGGAGCCTGTGCTCTCCTCTAGCTTGGCCCAACGCAGAG GCATGAAGAGACTCACGTCTACCCGGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_057128
Insert Size	2202 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_057128.1, NP_476469.1
RefSeq Size	5396 bp
RefSeq ORF	2202 bp
Locus ID	117269
Gene Summary	ribosomal protein; may be upregulated by the insulin signaling pathway in skeletal muscle [RGD, Feb 2006]

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