Dclk3 (NM_001191800) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Dcamkl3; RGD1309232
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217222 representing NM_001191800 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCGCGCCGGTCCTGCGCCCTCCGCCGCCACCGGCGACCCCTGCCCCGCCGGCACCCAGCCGCC CTGCGCCTGCTATCTCGGGCCATCGGGGTCCGTGTGACCATTCTCTAAAATGCTTAAGCTCGAAGATCTC TGAGAGAAAGTTGCCAGGCTCCTGGCTGCCTTCGGGACGAGGACCTCTGGAGAAGCCAGTCCTGGGGCCA CGTGGTGCCGTCATGCCGATGTTCAGCCCTCAGAGTAGCCTCCACTCAGTCCGCGCAGAGCACAGCCCAC TGAAGCCCAGGGTGGTTACGGTGGTGAAGCTGGGCGTGCAGCCCCTCCGCAAGGCCACCCTGCTCCTCAA CAGGCGGTCAGTGCAGACCTTTGAGCAGCTCCTGTCGGACATCTCTGAAGCCTTGGGCTTCCCGCGATGG AAGAATGACCGTGTGCGGAAGCTGTTCACCCTCAAGGGCAGGGAGGTCAAGAGTGTGTCTGACTTCTTCC GGGAGGGCGATGCTTTCATAGCCATGGGCAAAGAGCCACTGACATTGAAGAGTATCCAGTTGGCTATGGA GGAACTGTATCCTAAGAACCGCGCCCTTGCCCTGGCCCCTCATAGTAGAGTCCCCTCCCCAAGGCTAAGA AGCAGGCTTCCCAGCAAGCTTCTGAAAGGAAGTCACCGCTGTGGGGAGGCAGGAAGCTATGGTGAGGTAA TGGGGAACAAGGCAGTCATTAGGCACCAGGGCAAGACTTCCACAGAGCTGGCCCCAGAAGACAAGGCAAG GGCCCAGAAGAAGTGGGTAGGAGGGAAACAGGAATCAGAAAATGGTGGCCTGCCGTCACCTAGAGAAGCC ACTCAGGAGGAGACGCATGCAAGTGGGGAGAAGCACCTCGGGGTGGAGATTGAAAAGACCTCGGGGGAGA TTGTCAGGTGTGAGAAGTGTAAGAGAGAAACAGAGCTGCAGTTGGGCCTACAGAGGGGGCAGTGCCCTCC GGGAACCAGTGAGCTGGACCTGGGGAGGGGCCAGAAGAGGGATTCCGAGAAGTTGGTCAGGACCAAGAGC TGCAGGAGGGCTTCTGAGGCAAAGTCTACAGATGGAGAGGAAGGGTGGAAGGGTGACAGCCACCGGGGCA GCCCCAGGGATCCCCCTCAGGAACTGAGGAGGCCCAACAACAACTCAGACAAGAAAGAGAGCAGAGGCTC AGAAGCTCAGGAAAGTCACCCTCAGGGCGTGGCCAAGGCCCAGAAGGACCTCATGGAAGGTCTGCCAGCT GTAGAGGAGGGGGCGGTGGACGCGAGGAGAGATTCCAGGCACACATGCAGGATTAAGCATGCTGCCTGGC TCCGGAGAGAGCAGCAGGCTGAACCCCCACAGCTCCCCAGAACCCGAGGGGAGGAGAAGGAAGCAGAGCA TGAGAAGAAGTCAGGTGGTCTGGGAGGGAGGAGGATGCTAGAAAAGGAGTCTAAGACGAAGCCGGAAGAG AACCGGCCGGAACGACCCAGTGGCCGGAAGCTGAGGCCCACGGGCATCATCTCAGCGGATGTGGAGAAGC ACTATGACATAGGTCGGGTCATTGGGGATGGGAATTTTGCCATCGTGAAGGAATGCAAGCACCGAGAGAC CAGGCAGGCTTACGCCATGAAGATTATTGACAAATCCCAGCTGAAGGGTAAGGAGGACATTGTGGACAGT GAGATTTTAATTATCCAGAGTCTCTCTCACCCCAACATAGTGAAACTGCATGAGGTCTACGAAACAGAGG CAGAGATCTACCTGATCATGGAGTACGTGCAGGGAGGGGACCTTTTCGATGCCATCATCGAAAGTGTGAA GTTTCCAGAGCCTGACGCTGCAGTTATGATCACAGACTTGTGTAAGGCCCTCGTCCACATGCATGACAAG AAGATCGTCCACCGGGACCTGAAGCCGGAAAACCTCCTGGTTCAGCGAAATGAAGACAAGTCTACCACCT TGAAGCTGGCTGACTTTGGCCTGGCCAAACATGTGGTGAGGCCTATATTTACTGTGTGTGGGACGCCAAC ATATGTAGCTCCTGAGATTCTTTCTGAGAAAGGTTACGGCCTGGAGGTGGACATGTGGGCCGCAGGTGTG ATCCTATACATCCTCTTGTGTGGCTTCCCTCCTTTCCGAAGTCCTGAGAGGGACCAAGACGAGCTCTTCA ACATCATCCAGCTGGGCCAGTTTGAGTTCCTCTCTCCTTACTGGGACAACATTTCTGATGCTGCCAAAGA TCTGGTGAGAAATTTGCTGGTAGTGGACCCCAAGAAGCGGTACACGGCCCACCAGGTCCTACATCATCCC TGGATTGGGATGGTGGGGCACCCCAGCACAGTGAACCCACAGAAGGAGGAGTCCCCCAGCAGCGAGGGTC GCTTCCAGAGCCAGCACAAGAAGGTTGCAGAGCACGTGTCGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191800
Insert Size	2424 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191800.1, NP_001178729.1
RefSeq Size	3249 bp
RefSeq ORF	2424 bp
Locus ID	316023
Gene Summary	This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

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