Sidt1 (NM_001100653) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217243 representing NM_001100653 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGACTGCCCGCGGCTGGCGCTGCTCTGCGCGCTGCCCTGGCTCCTACGGGCGGCGGTCCCTGGCC ACCGGGCAGAGCCCCTCAAGCGGTCGGCAGAGCTGCCCCGCAATCCGCGCGACCCCGCCAGAGGAGCGGA TTTCGATCGCGTCTACAGCGGCGTGGTGAGTCTCAGCACCGAGAACATCTACTCCTTCAACCACACCAGC CATCCTGGCCAGGTGACAGCCGTGCGGGTGCATGTCAACAGTTCCTCTGACAACCTGGACTACCCAGTCC TGGTTGTGGTTCGCCAACAGAAACAAGTGTTGTCCTGGCAGGTTCCTCTGCCCTTCCAAGGACTATACCA AAGGAGCTACAACTACCAGGAAGTCAGCCGCACTCTATGTCCTTCTAAAGCAACCAATGAGACCGGCCCT TTGGAACAGCTGATCTTTGTCGACGTAGCATCCATGGCTCCCCACGGTGCCCACTACAAGTTGCTGGTCA CCAAAATCAAGCACTTCCAGCTCCGGACGAATGTTGCCTTCTACTTCACTGCCAGCCCCTCTCAACCTCA GTATTTTCTATACAAATTTCCTGAAGATGTGGACTCCGTTATCATTAAAGTGGTGTCTGAGAAGGCTTAT CCGTGTTCAGTTGTCTCCGTTCAGAACATCATGTGTCCAGTGTATGATTTAGACCATGATGTGGAATTCA ATGGTGTCTATCAGTCCATGACCAAACAAGCTGCCATCACGCTGCAGAAGGATTTTCCAGATGAGCAGTT CTTCGTGGTGTTTGTGATAAAGCCTGAAGATTATGCATGTGGTGGATCATTCTCCATCCAAGAAAATGAA AATCAGACCTGGAACCTACAACGATCAAAGAACCTGAAAGTGACCATTGTCCCATCTGTCAAAGGATCTG TGTATGTGAAATCTAGTCTTTTCAGCGTATTCGTTTTCTTGTCCTTCTACTTGGGCTGCCTGCTGGTCGT GTTTGTCCATCACATGAGGTTCCAGAGGAAACCCGTTGATGGAAGCTTTGGCTCCGGTGATGGCTCTGGA AATATGGCTGTCTCACATCCCATCACGGCCAGTACACCTGAAGGGAGCAATTATGGGGCAATAGACGAGT CGAGTTCCAGTCCTGGAAGGCAGATGTCTTCTTCCGATGGTGGGCAGCCATGCCACTCAGACACGGACAG CTCTGTGGAGGAGAGTGACTTCGACACTATGCCAGACATCGAAAGTGACAAAAACGTCATCCGGACCAAG ATGTTCTTGTACCTGTCTGATCTGTCCAGGAAAGACCGGAGGATTGTCAGCAAAAAATATAAGATTTATT TTTGGAATATCATCACCATCGCTGTGTTTTATGCCCTGCCTGTGATGCAGCTGGTCATCACCTACCAGAC AGTGGTAAATGTCACTGGCAACCAGGACATCTGTTACTACAACTTCCTCTGTGCTCACCCCCTGGGCGTC CTGAGCGCCTTCAATAATATTCTCAGTAACCTGGGCCACGTCCTCCTGGGCTTCCTCTTCCTGCTGATAG TGCTGCGCCGTGACCTTCTCCACCGAAGAGCCCTGGAGGCTAAAGACATCTTTGCTATGGAGTATGGAAT TCCCAAACACTTTGGTCTCTTCTACGCCATGGGGATCGCGCTGATGATGGAAGGCGTGCTCAGTGCTTGT TACCATGTCTGCCCTAATTACTCCAACTTCCAGTTCGACACCTCCTTCATGTACATGATTGCCGGCCTCT GCATGCTGAAGCTCTACCAGACCCGCCACCCGGACATCAATGCCAGCGCCTACTCTGCCTATGCCTCCTT CGCTGTGGTCATCACACTCACGGTCCTCGGAGTGGTATTTGGAAAAAACGATGTGTGGTTCTGGATCATC TTCTCCGCTATCCACGTCCTTGCTTCTCTGGCCCTCAGCACCCAAATCTACTACATGGGCCGTTTCAAGA TAGATGTGTCCGACACAGATCTGGGAATCTTCCGGCGCGCTGCTATGGTGTTCTACACGGACTGTATCCA GCAATGCAGCAGACCTCTGTATATGGACAGGATGGTGTTGCTCATTGTGGGAAATCTGGTTAACTGGTCC TTTGCCCTCTTTGGATTGATCTACCGGCCCAGGGACTTTGCCTCCTACATGCTGGGCATCTTCATCTGCA ACCTGCTGCTCTACTTGGCTTTCTACATCATCATGAAGCTTCGAAGCTCTGAGAAGGTCCTCCCCCTACC AGTCTTCTGCATCGTGGCCACTGCTGTGGTGTGGGCTGCGGCCCTGTACTTTTTCTTCCAGAACCTCAGC AGCTGGGAGGGAACTCCAGCCGAATCCCGGGAGAAGAACCGAGAGTGTGTCCTGCTGGGTTTCTTTGATG ACCATGACATCTGGCACTTCCTTTCTGCTACTGCCCTGTTTTTCTCATTCCTGGTGTTGTTGACTCTGGA TGACGACCTTGATGTGGTCCGCAGAGACCAGATCCCTGTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100653
Insert Size	2496 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100653.1, NP_001094123.1
RefSeq Size	3928 bp
RefSeq ORF	2496 bp
Locus ID	288109
UniProt ID	Q6Q3F5
Gene Summary	In vitro binds long double-stranded RNA (dsRNA) (500 and 700 base pairs), but not dsRNA shorter than 300 bp. Not involved in RNA autophagy, a process in which RNA is directly imported into lysosomes in an ATP-dependent manner, and degraded.[UniProtKB/Swiss-Prot Function]

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