Trim71 (NM_001191801) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1566388
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217265 representing NM_001191801 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGTTCCCTGAGACCGACTTCCAGATCTGCCTGCTGTGCAAGGAGATGTGCGGCTCGCCCGCGC CTCTCTCCTCCAGCTCGTCCGCCTCTTCATCGTCCTCGCAGACGTCCACGTCGTCGGCGGGCGGCGGCGG CCCCGGGGCGGCGGCGCGGCGCCTGCACGTCCTGCCCTGCCTGCACGCCTTCTGCCGGCCGTGCCTCGAG GCGCACCGGCTCCCGGCTCCCGGTGGCGCCGGCCCCGCCGAGGCGCTGAAGCTGCGCTGTCCTGTGTGTG ACCAGAAGGTGGTCCTGGCCGAGGCGGCAGGCATGGACGCGCTGCCGTCGTCCGCCTTCCTGCTCAGCAA CCTGCTGGACGCCGTGGTGGCCACCGCCGACGAGCCGCCGCCCAAGAACGGGCGCGCGGGTGGCGGCCCC GGGGGCGCGGGCGGCCACAGCAATCACCGGCACCACGCGCACCACCCGGCCCAGCGCGCCGCCGCTCCAG CGCCGCAGCCGCCGCCCGGCCCGGCCGCTTCTCCAGGGTCGCTGTTGTTGCGCCGGCCGCACGGCTGCAG CTCCTGCGACGAGGGCAATGCTGCCTCGTCGCGCTGCCTCGACTGCCAGGAACACCTGTGCGACAACTGT GTCCGCGCGCACCAGCGCGTACGCCTCACCAAGGACCACTACATCGAGCGCGGCCCGCCGGGGCCTGCGG CCGCCTCCGCGGCGCAGCAGCTGGGGCTCGGGCCGCCCTTCGCAGGCGCGCCCTTCTCCATCCTCTCGGT GTTCCCGGAGCGCCTCGGCTTCTGCCAACACCACGACGACGAGGTACTACATCTGTACTGCGACACCTGC TCCGTGCCCATCTGCAGAGAGTGCACACTGGGCCGCCATGGCGGCCACAGCTTCGCCTACCTCCAGGACG CCCTACAGGACTCACGGGCACTCACCATCCAGCTGCTGGCCGACGCCCAGCAGGGCCGCCAGGCCATTCA GCTGAGCATTGAGCAGGCGCAGACTGTGGCAGAACAAGTGGAGATGAAGGCCAAGGTGGTCCAGTCGGAG GTCAAGGCCGTCACGGCGAGACACAAGAAAGCTCTGGAGGAGCGTGAGTGCGAGCTGCTGTGGAAGGTGG AGAAGATCCGGCAGGTGAAGGCTAAGTCTCTCTACCTGCAGGTGGAGAAGCTGCGGCAGAATCTCAACAA GCTGGAGAGCACCATCAGCGCCGTCCAGCAGGTCCTAGAAGAGGGCCGAGCCCTGGACATCCTGCTGGCC CGAGACCGGATGCTGGCTCAGGTGCAGGAGCTCAAGACCATCCGGGGCCTCCTGCAGCCCCAGGAAGATG ACCGAGTTATGTTCACACCCCCCGACCAGGCCCTGTACCTTGCCCTCAAGTCTTTTGGCTTTGTCAGCAG TGGGGCCTTTGCCCCACTCACGAAGGCCGCGGGAGATGGCATCAAGCGAGCCCTTCAGGGAAAGGTGGCC TCCTTCACTGTCATGGGCTATGACCACAATGGTGAGCCCCGCCTCTCAGGAGGTGACCTGATGTCGGTGG TGGTCCTTGGCCCTGATGGAAACCTGTTTGGTGCAGAAGTGAGCGACCAGCAGAATGGGACCTACGTCGT GAGCTACAGGCCCCAGCTTGAGGGTGAACACTTGGTATCAGTGACGCTGTATAACCAGCACATCGAGAAC AGCCCCTTCAAGGTAGTGGTCAAGTCGGGCCGCAGCTATGTGGGCATCGGGCTCCCAGTGTTGAGCTTTG GCAGCGAGGGTGATGGCGAGGGTAAGCTCTGCAGGCCCTGGGGCGTGAGTGTGGATAAGGAGGGCTACAT CATCGTGGCTGACCGCAGCAATAACAGAATTCAGGTGTTTAAGCCCTGTGGGTCTTTCCACCACAAGTTT GGTACCCTAGGATCTCGGCCCGGGCAGTTTGACCGACCGGCGGGGGTGGCCTGCGACGCCTCCCGCAGGA TCGTGGTGGCCGACAAAGACAATCATCGCATCCAGATCTTCACCTTCGAGGGCCAGTTCCTTCTGAAGTT TGGCGAGAAAGGAACCAAGAATGGGCAGTTTAACTACCCGTGGGATGTAGCGGTCAACTCTGAGGGCAAG ATCCTGGTTTCAGACACCCGAAACCACCGCATCCAGCTGTTCGGACCCGACGGCGTCTTCCTGAATAAGT ATGGCTTCGAGGGATCTCTCTGGAAGCACTTCGACTCCCCACGGGGCGTGGCTTTCAACCATGAAGGTCA CCTGGTAGTCACTGATTTCAACAACCATCGGCTCCTGGTTATCCACCCCGATTGCCAGTCTGCCCGCTTC CTGGGCTCTGAGGGCACTGGCAATGGGCAGTTCCTGCGACCACAGGGTGTGGCTGTAGACCAGGAAGGGC GAATCATTGTCGCCGATTCCAGAAATCACCGTGTGCAGATGTTTGAGGCCAACGGCAGCTTCCTGTGCAA GTTCGGTGCTCAAGGCAGCGGCTTCGGGCAGATGGACCGCCCCTCAGGTATCGCGGTCACCCCGGAAGGA TTGATCGTCGTGGTGGACTTTGGCAACAATCGAATCCTCATCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191801
Insert Size	2568 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191801.1, NP_001178730.1
RefSeq Size	2568 bp
RefSeq ORF	2568 bp
Locus ID	301042
UniProt ID	D3ZVM4
Gene Summary	E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance (By similarity). Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A (By similarity). In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism (By similarity). Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity). Specific regulator of miRNA biogenesis. Binds to miRNA MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression (By similarity).[UniProtKB/Swiss-Prot Function]

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