Pkd2 (NM_001191934) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1559992; Trpp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217374 representing NM_001191934 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTCCAGCCGTGTGCAGCCGCAGCCTCCCGGGGACCCCGGACGCTCGCCCGCGCCGCGAGCGT CCGGGCCCGGCCGCCTGCTGGCGGGAGGCGCCGGCCTGGCAGTCCCCGGCGGCCTGCGGGAGCAGCGGGG CCTGGAGATTGAGATGGAGCGCATCCGGCAGGCGGCCGCTCGGGACCCACCGGCCGGAGCCTCGGCCTCG CCTTCTCCTCCGCTGTCGTCCTGCTCCAGGCAGGCATGGAGCCGCGACAACCCGGGCTTTGAGGCCGAAG AGGATGACGACGACGACGAGGTGGAAGGAGAAGAAGGAGGGATGGTGGTGGAGATGGACGTGGAGTGGCG TCCGGGCAGCCGAAGACCGGCCTCCTCTTCGGCCGTGAGCTCGGCGGGCGCCCGCGGCCGGGGTCTGGGA AGCTATCGCGGCGCGGCTTACCCGAGCGGGAGACGGCGCCGGCTAGAGGACCAGGGCGCGCCGTGTCCCA GCCCCGCGGGCGGCGGGGACCCGCTGCATCGCCACCTCCCGCTGGATGGCCAGCCGCCCCGAGTGGCCTG GGCCGAGAGGCTGGTGCGAGGACTGCGAGGTCTCTGGGGAACAAGACTCATGGAAGAGAGCAACGCTAAC CGAGAGAAATACCTGAGAAGCGTGTTGCGGGAGCTGGTCACTTACCTCTTTTTCCTCATACTCTTGTGCA TCTTGACCTATGGCATGATGAGCTCCAACGTGTACTACTACACTCGGACACTGTCACAACTTTTCATAGA CACCCCAGTGTCTAAAACAGAGAAAACCAACTTTAAAACTCTCTCTTCCATGGAGGACTTCTGGAAGTTC ACAGAAGGCTCCTTCCTGGATGGGCTGTACTGGAAGGCACAGAGCAGCAACCATACGCAAGCTGACAACC GGAGCTTTATTTTCTATGAGAACCTGCTGCTAGGAGTGCCGCGTCTACGCCAACTCCGAGTCAGAAACGG ATCCTGCTCCATCCCTCAGGACCTGAGAGATGAAATTAAAGAATGCTATGATGTCTACTCTGTCAGCAGT GAGGACAGGGCTCCATTTGGACCGCGGAACGGAACTGCGTGGATCTACACAAGTGAAAAAGACCTGAATG GCAGCAGTCACTGGGGAATCATTGCGACTTACAGTGGAGCCGGTTACTACCTGGATCTGTCCAGAACCAG GGAGGAGACAGCGGCCCAGCTTGCTGGCCTCAGGAGGAATTTCTGGCTGGACCGGGGCACACGGGCAGCT TTCATTGACTTCTCGGTGTATAACGCAAACATTAACCTGTTCTGTGTGGTCAGGTTATTGGTTGAGTTCC CAGCAACGGGTGGCGTGGTGCCCTCTTGGCAGTTTCAGCCTGTAAAACTGATCCGCTACGTCACAGCCTT TGATTTCTTCCTGGCAGCCTGTGAGATTATCTTTTGTTTCTTTATCCTTTACTATGTGGTGGAAGAGATA TTGGAAATTCGGATTCACAGACTGAACTATTTCAGGAGTTTCTGGAATTGTCTGGATGTTGTGATTGTTG TGTTGTCCGTAGTAGCCATGATGATTAACATTTACCGGATGTCCAATGCTGAGGGGCTGCTACAGTTTCT CGAAGATCAAAATTCTTTCCCCAACTTTGAGCATGTGGCATACTGGCAAATACAGTTCAACAATATAGCT GCCGTCATGGTATTTTTGGTCTGGATTAAGCTCTTCAAATTCATCAATTTCAATAGGACCATGAGTCAGC TCTCCACGACCATGTCCAGATGTGCCAAAGACCTCTTTGGCTTCACCATCATGTTCTTCATCATCTTCTT GGCATATGCCCAGTTGGCGTACCTTGTCTTTGGCACTCAGGTGGATGACTTCAGTACTTTCCAAGAGTGT ATTTTCACTCAGTTCCGCATCATTTTGGGTGATATCAACTTCGCAGAGATCGAGGAAGCTAACCGAGTTT TGGGGCCACTTTATTTTACTACATTTGTGTTCTTTATGTTCTTCATTCTTTTGAACATGTTTCTGGCGAT CATCAATGATTCCTACTCTGAGGTGAAATCAGATCTGGCCCAGCAGAAAGCAGAAATGGAACTCTCAGAC CTTATCAGAAAGGGCTGCCAAAAAGCACTTGTCAAACTAAAACTTAAAAGAAACACCGTAGACGCCATTT CAGAGAGTCTCCGGCAAGGTGGTGGCAAGCTAAACTTTGATGAACTTCGGCAAGACCTGAAAGGGAAGGG CCACACGGACGCAGAGATCGAGGCCATATTCACTAAATATGACCAGGACGGCGACCAGGAACTGACTGAG CGTGAGCATCAGCAGATGAGAGATGACTTGGAGAGAGAGAGGGAGGACCTAGACTTGGAACACAGCTCTT TACCACGTCCCATGAGCAGCAGAAGTTTCCCCAGAAGCCTGGATGACTCTGAGGAGGAAGACGATGAAGA CAGTGGCCATAGTTCCAGGAGGAGGGGAAGCATCTCCAGTGGGGTTTCCTATGAAGAGTTCCAAGTACTG GTGAGACGCGTGGACCGCATGGAGCACTCCATCGGCAGCATCGTGTCCAAGATCGATGCTGTGATTGTCA AGCTTGAGATCATGGAGCGGGCCAAGGTGAAGAGGCGAGAGGTGTTAGGAAGGCTGCTGGATGGAGTGGC TGAGGATGCGCGCCTGGGTCGGGATAGTGAGATCCACAGGGAGCAGATGGAGCGCCTGGTGCGGGAAGAG CTGGAGCGCTGGGAATCGGATGACGCAGCTTCACAGTCAGGTCACGGTCTAGGCACACAAGTGGGACTCG GTGGCCAGTCGCACCCCAGAAGCTCCCGGCCCCCTTCCTCCCAGTCTGCAGAGGGCCTGGAAGGCGGAGG TGGAAACGGAAGTACCAACGCGCATGCGTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001191934
Insert Size	2901 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191934.1, NP_001178863.1
RefSeq Size	4851 bp
RefSeq ORF	2901 bp
Locus ID	498328

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