Atp1a4 (NM_001271030) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217422 representing NM_001271030 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTGGAAAAGAGACAGCGGCCACTAGTGAGCAGAAGCCAAGACCCACCCTTAGGGCTTCAAATA CGAATAGACAGCCGAAAGTGAAAAGAAGGAAGAAAGATTTAGAGGAGTTGAAGAAGGAAGTGGTTATGGA CGATCACAAGCTAACGTTGGATGAGCTGAGTGCCAAGTACTCTGTGGACTTGACCAAGGGTCTTAGCGTC ACAGATGCCCAGGAAATTCTGACTCTAAATGGACCGAATGTACTAACCCCTCCTCCAACCACTCCAGAAT GGATCAAATTCTGTAAACAATTATTTGGTGGCTTCTCCCTCCTGCTGTGGACTGGTTCCTTGCTCTGCTT CCTGGCCTATGGCATACATGTATCTTATTACCAAGAAAACGCCAACAAAGATAATCTGTACCTGGGTATT GTACTATCTGCCGTGGTCATCATCACTGGCTGCTTCTCATATTACCAGGAGGCGAAGAGCTCAAAGATCA TGGAGTCTTTTAAGACCATGGTGCCTCAGCAAGCTCTGGTAATTCGAGATGGAGAGAAAATGCAGATCAA TGTAAGAGATGTGGTTTTGGGAGACCTAGTAGAAGTGAAGGGTGGAGACCAAGTCCCTGCTGATATCAGG GTTATCGCTGCGCAAGGATGTAAGGTAGACAATTCCTCCTTGACTGGGGAGTCAGAACCACAGTCTCGTT GCCCTGATTGCACCCATGAGAACCCTCTGGAGACACGGAATATCATCTTCTTCTCCACCAACTGCGTAGA AGGAACAGCCCGGGGTGTTGTGATTGCTACAGGAGACCACACAGTAATGGGCCGGATTGCTTCCCTGACA TCAGGATTGACAATGGGCAAGACCCCGATTGCCACTGAAATCGAACACTTCATCCATATAATCACAGCTG TCGCAGTCTTCCTCGGTGTCACGTTTTTTTTCCTCTCACTTATCTTGGGCTATACCTGGCTGGATGCTGT CATTTTCCTTATTGGCATCATTGTGGCCAATGTGCCAGAAGGGCTGCTGGCCACTGTCACTGTGTGCCTG ACGCTGACAGCCAAGCGCATGGCTCGCAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGACGCTGG GCTCCACGTCCACCATCTGCTCGGACAAGACTGGCACCCTCACCCAGAACCGCATGACGGTGGCTCATCT GTGGTTTGATAAGACTGTGTATGAGGCTGACACCAGCGAGGAGCAAACCACGGGGAAAACATTTCCCAAG TCCTCTGACACCTGGTTTTACCTGGCTCGAATTGCTGGCCTCTGCAACCGGGCCGACTTTAAGCCTCATC AGGAGTCTCTTCCCATAACTAAGCGGACAACAACAGGGGATGCTTCTGAGTCAGCCCTCCTCAAATTCAT TGAGCAGTCTTACAGCCCTGTGAGTGAAATGAGACAGAAAAACCCCAAGGTGGCAGAGATTCCTTTTAAT TCTACCAACAAGTACCAGATGTCTATTCACCTTCTGGAAGATAACTCAGAAGCCCACGTGCTGCTGATGA AAGGTGCTCCAGAAAGGATCTTGGATTTTTGCTCCAGTTTCCTGCTCAATGGGCAGGAGTACCCTATGGA CGAAGAAATGAAGACAGACTTCCAGAATGCCTACATAGAACTGGGAGGTCTGGGCGAGCGTGTGCTGGGG TTCTGCTTCTTGAATTTGCCTAGCAATTTCTCTAAGGGATTCCAATTTAATACCGAAGAATTAAATTTTC CCATGGAGAATCTGTGTTTCGCCGGCCTCATATCTATGATCGACCCTCCTCGAACGGCTGTGCCTGATGC TGTTAGCAAGTGCCGAAGTGCAGGGATTAAGGTAATCATGGTAACAGGGGATCATCCCATCACAGCCAAA GCTATTGCCAAAAGTGTGGGCATCATTTCAGAAGCTAATGAAACGGCGGAGGACATCGCTGCTCGGCTTA ATATCTCCATCAGTCAAGTCAGTAACAAGTCTATTAAAGCCATTGTAGTGCACGGCTCAGAGCTGAAGGA CATGGACTCAGGTCAGCTGGACAATATCCTCAAGTCCTACAAGGAGATCGTGTTTGCGCGGACCTCTCCT CAGCAGAAGCTCATCATCGTGGAGGGCTGTCAGAGACTGGGAGCCATTGTGGCAGTGACTGGTGACGGGG TGAACGACTCCCCCGCGCTGAAGAAGGCTGACATCGGCATTGCCATGGGCATCACTGGCTCTGATGTCTC TAAGCAGGCGGCTGACATGATCCTTCTCGACGACAACTTTGCCTCCATTGTGACGGGCGTGGAGGAGGGT CGCCTCATCTTTGACAACCTGAAGAAGTCCATCGCCTATACCCTGACCAGCAACATCCCTGAGATCACCC CCTTCCTGCTGTTCATCGTCTTAAGTATACCCTTGCCTCTGGGTACCATTACCATTCTCTGCATTGACCT GGGTACTGACATGGTCCCTGCTATCTCCTTGGCTTATGAGACACCTGAAAGTGACATCATGAAAAGGCTT CCACGGAACCCCAAAACTGACAATCTCGTGAACGACCGTCTTATCGGCATGGCCTATGGGCAGATCGGGA TGATACAGGCGCTGGCTGGATTTTTCACCTATTTCGTGATCCTGGCGGAGAATGGTTTTAAGCCTCTTGA TCTTCTGGGTATCCGTCTATACTGGGATGATACAAACCTAAATGACCTAGAGGACACCTATGGGCAGCAG TGGACCTATGAGCAGCGGAAGGTGGTGGAGTTCACATGCCAAACAGCCTTCTTCATCAGCATCGTGATCG TGCAGTGGGCTGACCTCATCATCTGCAAGACGCGCAGAAATTCACTCTTCAAGCAGGGCATGAAAAACAA AGTCCTAATATTTGGACTCCTTGAGGAGACGATCCTGGCAGCTTGCCTGTCCTACATTCCAGGCATGGAT GTAGCCCTGAGAATGTACCCACTCAAGATAAACTGGTGGTTCTGCGCACTTCCCTACAGTGTTCTCATCT TCATCTATGATGAAGTTCGAAAACTCATCATCCGCAGACGGCCCGGTGGATGGCTGGAGAAGGAGACGTA CTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271030
Insert Size	3087 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271030.1, NP_001257959.1
RefSeq Size	3491 bp
RefSeq ORF	3087 bp
Locus ID	29132
UniProt ID	Q64541
Gene Summary	alpha subunit isoform of Na+, K(+)-ATPase, which has sodium/potassium ion exchange activity; may be involved in regulation of intracellular hydrogen ion homeostasis and sperm motility [RGD, Feb 2006] Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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