Rbl1 (NM_001191066) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217437 representing NM_001191066 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGAGGACGAGCCCCACGCCGAGGGGGCGGCAGCCGTCGCCGCGGCCAGGGAGGCGCTGCAGGCCC TGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCCGCCGAAGCCCTGGACGACTTCACCGCCATCCGCGG CAACTACAGCCTAGAGGGGGAAGTTATACACTGGCTGGCATGCTCTTTGTACGTCGCTTGCCGCAAGAGC ATCATTCCTACCGTGGGAAAGGGCGTCATGGAAGGAAACTGTGTTTCGCTGACCAGAATACTACGCTCAG CTAAGTTAAGCTTAATTCAGTTTTTTAGTAAAATGAAGAAGTGGATGGACATGTCAAACCTACCACAAGA ATTCCGGGAGCGTATAGAAAGGCTAGAAAGAAATTTTGAAGTATCTACAGTAATTTTTAAAAAATTTGAG CCCATTTTTTTAGATATCTTTCAAAATCCATATGAAGAGCTACCAAAGTTGCCACGAAGCAGAAAGCAGA GGAGGATTCCTTGCAGTGTTAAGGATCTCTTTAATTTCTGCTGGACGCTCTTCGTTTACACTAAGGGTAA TTTTCGTATGATTGGTGATGATTTAGTAAACTCATATCATTTACTTCTGTGCTGCTTGGACCTGATTTTT GCCAATGCTATAATGTGTCCAAATAGACGAGACTTGTTAAATCCATCATTTAAAGGTTTACCATCGGATT TCCATGCTGTGAACTTCAAAGCTGCAGAAGAACCACCCTGTATCATTGCTGTACTTTGCGATCTGCACGA TGGACTTTTAGTAGAAGCAAAAGGAATAAAGGAGCACTACTTCAAACCATATATTTCAAAACTCTTTGAC AAGAAGATTTTAAAAGGTGAATGTCTCTTGGATCTTTCCAGTTTTACTGATAATAGCAAAGCAGTGAACA AGGAGTATGAAGAGTATGTTCTAACTGTTGGGGACTTTGACGAGAGGATCTTTTTGGGAGCAGATGCAGA GGAGGAGATCGGAACACCTCGAAAGTTCGCTGCTGACACCCAATTTGGGAAACTGACATCACAGGCCAGT GTGGACTGCAACCTTCAACAACACTTTGAAAAAAAACGGTCATTTGCACCTTCTACCCCACTTACTGGAC GGCGCTATTTACAAGAAAAAGAGGCAGTTACCACACCTGTTGCTTCAGCTACTCAGAGTGTAAGCCGATT ACAGAGCATTGTAGCCGGATTGAAAAGTGCCCCGAGCGAGCAGCTTCTGACTATTTTTGAATCTTGTATG CGGAATCCGATGGGAAACATTGTAAAAATAGTGAAAGGAATAGGAGAGACTTTCTGCCAGCACTATACCC AGTCCACAGATAAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGACTGAAACTGGCAGAAATTTT GTATTATAAAATACTAGAGACTATAATGGTCCAGGAAACACGACGACTTCATGGAATGGACATGTCAGTT CTTTTAGAACAAGACATATTTCACAGGTCCTTGCTGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATA GCTCACCCCGTACTTTCCCTTGGATCATTGACGTTCTCGGTTTGCAGCCGTTTTACTTCTATAAGGTTAT TGAGGTGGTGATCCGCTCAGAGGAGGGGCTCTCCAGAGACATGGTGAAACACCTAAACAGCATTGAAGAG CAGATTTTGGAGAGTTTAGCTTGGACTAATAATTCTGCACTGTGGGAAGCTCTCCGTGCTTCTGCAAATA AAGTTCCTTCCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAATAGGAAATGGAGGAAGTGTGCAAGG CCATCTTCCCATGATGCCAATGTCTCCAATAATACATCCAAGAGTCAAGGAAGTTCGCACTGACAGTGGG AGCCTTCGAAAAGATATGCAGCCACTGTCTCCCATCTCCGTCCATGAGCGCTACAGCTCCCCTGCCGCAG GAAGTGCTAAGAGGAGACTCTTTGGTGATGACCCACCAAAGGAGACATTGATGGAAAAGATTATGGCAGA AGGAACACAGCTGAAAATTGCTCCTTCAAGTGTGACTGCTGAAAGCTTGTCAATTTCCCCTGGGCAAGCT CTTCTCACAATGGCCACGACCACAGTCACAGGGACGACGGGACGGAAGGTTACCGTTCCTTTGCATGGTA TTGCCAATGATGCTGGAGAAATCACACTGGTTCCTATTTCCATGAATACAACTCAGGACTCCACAGCTGA GAGCCTTGTATCACTAACTGCACAGTCATTAATTGGTGCTTCTCCAAAACAGACCCATCTGACTAAAACG CAAGACGCTCCTCTGACCGGAATAAGCAAACCAAAGAGAACTGGGTCCTTAGCACTGTTTTATAGAAAGG TCTATCATTTGGCAAGTGTACGCTTACGTGATTTATGTTTAAAACTTGATGTTTCGAATGAGTTACGAAG GAAGATCTGGACATGTTTTGAATTCACTTTAGTTCACTGCCCTGATTTAATGAAAGATAGGCATTTGGAT CAGCTCCTTCTGTGTGCCTTTTACATCATGGCCAAAGTAACAAAAGAAGAAAGAACTTTTCAAGAAATAA TGAAAAGTTACAGAAATCAGCCACAAGCTAATAGTCACGTATACAGGAGTGTTCTCTTGAAAAGTATTCC AGGAGAAGTTGTGGCATACAATGGTGACTATGAGATGACTGATGGTGACATAGAAGATGCCACAAAAACT CCCAACTGTTCCAGTGAACCAGTGAAAGAGGAAAGAGGTGATCTTATCAAATTCTACAATGCGATATATG TAGGAAGAGTGAAGTCATTTGCATTGAAGTATGATTTGTCCAATCAGGACCATATAATGGATGCTCCACC GCTCTCTCCTTTCCCACATATTAAGCAGCAGCCGGGCTCCCCACGCCGCATTTCTCAGCAGCATTCCATT TATGTCTCTCCGCACAAGAACGCATCAGGCCTCACCCCCAGGAGCGCACTACTCTACAAGTTCAACGGCA GCCCCTCTAAGAAGGCCAAGAAGCGTGTGATCGCCATCAGCGGAGATGCAGAGTCACCTGCCAAACGTCT CTGCCAGGAGAACGATGATGTTTTACTTAAACGACTGCAGGATGTTGTCAGTGAAAGAGCGAATCATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191066
Insert Size	3150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191066.1, NP_001177995.1
RefSeq Size	3150 bp
RefSeq ORF	3150 bp
Locus ID	680111
UniProt ID	D3ZS28
Gene Summary	Key regulator of entry into cell division (By similarity). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity). Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]

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