Ankrd52 (NM_001191875) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1307124
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217457 representing NM_001191875 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATCCTCAGCATCACGGACCAGCCGCCCCTGGTCCAGGCCATCTTTAGCCGAGATGTGGAGGAAG TGCGTTCCCTCCTCTCGCAGAAGGAGAACATCAATGTACTGGACCAAGAGAGGCGAACCCCATTGCATGC TGCTGCCTACGTAGGCGATGTCCCCATCCTCCAGTTGCTACTGATGTCAGGTGCTAATGTCAACGCTAAG GACACACTGTGGCTGACCCCTCTTCATCGTGCCGCTGCCTCCCGAAACGAGAAGGTGCTGGGGCTGCTGC TGGCACACTCCGCGGATGTGAATGCCCGGGACAAGCTGTGGCAGACACCACTGCATGTGGCTGCTGCCAA CCGGGCCACCAAGTGTGCCGAGGCTCTGGCACCTCTGTTGAGCAGCCTCAATGTGGCTGACAGGAGCGGC CGTAGCGCTCTGCACCACGCAGTACACAGTGGGCATCTTGAGACCGTGAACCTGCTCCTCAATAAGGGAG CCAGCCTGAATGTCTGCGATAAAAAGGAGCGGCAACCACTGCACTGGGCTGCTTTTCTAGGGCATTTAGA GGTCCTGAAACTGCTGGTAGCACGTGGAGCAGACCTCAGCTGTAAAGATCGGAAGGGCTACGGGCTGCTC CACACAGCTGCAGCCAGCGGCCAGATCGAAGTGGTGAAGTACTTGCTTCGGATGGGGGCTGAGATTGATG AGCCCAACGCTTTCGGAAACACAGCTTTGCACATCGCTTGCTACCTGGGCCAAGATGCTGTGGCTATCGA ACTAGTGAATGCAGGAGCCAATGTCAACCAGCCAAATGACAAGGGTTTCACACCACTGCACGTGGCAGCT GTCTCGACCAATGGCGCACTCTGTTTGGAGCTGTTAGTCAATAATGGGGCTGACGTCAACTACCAGAGTA AAGAAGGGAAAAGTCCCCTGCACATGGCTGCCATCCATGGGCGGTTCACGCGTTCCCAGATCCTCATCCA GAATGGTAGCGAGATTGATTGTGCTGACAAATTCGGGAACACGCCCCTACATGTGGCTGCTCGATACGGA CACGAGCTTCTCATCAGCACTCTCATGACCAATGGCGCAGATACTGCCCGGCGCGGTATCCACGACATGT TCCCCCTGCACTTAGCCGTTCTCTTTGGATTCTCTGACTGTTGCCGGAAGCTTCTTTCCTCAGGTCAGCT GTACAGCATCGTGTCTTCACTCAGTAATGAGCATGTGCTTTCTGCTGGGTTTGACATCAACACACCTGAC AGCCTTGGCCGCACCTGTCTTCATGCTGCTGCTTCTGGAGGGAATGTTGAGTGTCTTAATTTGCTGCTGA GCAGTGGAGCTGACCTGAGGAGGAGAGACAAATTTGGAAGGACCCCACTGCACTATGCAGCTGCCAATGG CAGCTACCAGTGTGCGGTGACACTGGTGACAGCCGGGGCAGGCGTCAATGAAGCCGACTGTAAGGGCTGC TCTCCCCTACACTATGCTGCTGCCTCTGACACCTACCGGAGAGCGGAACCCCACACTGCTTCCAGCCATG ATGCAGAAGAGGATGAGCTACTGAAGGAATCCCGTAGGAAGGAGGCCTTCTTCTGTCTGGAGTTCTTATT GGATAATGGTGCAGACCCCTCCCTGCGGGACAGGCAGGGCTACACAGCTGTGCACTATGCAGCCGCCTAC GGCAACAGACAGAACCTCGAACTGCTCTTAGAAATGTCCTTTAACTGCCTGGAGGATGTGGAGAGTACAG TTCCCGTCAGCCCTTTGCACTTAGCTGCCTACAACGGTCACTGTGAAGCCCTGAAGACACTGGCTGAAAC GCTGGTGAACCTGGATGTAAGGGACCACAAGGGCCGGACTGCACTCTTCCTGGCCACTGAGCGAGGCTCC ACTGAGTGTGTGGAGGTACTGACGGCCCATGGTGCCTCTGCCCTCATCAAGGAGCGTAAACGCAAGTGGA CACCCTTACATGCTGCTGCTGCCTCTGGCCACACCGATTCCCTGCACTTGCTGATTGACAGTGGGGAGCG CGCTGATATCACAGATGTCATGGATGCCTATGGACAAACCCCGCTGATGCTGGCCATTATGAATGGTCAT GTGGACTGTGTACATCTGCTGCTAGAGAAAGGATCCACGGCTGATGCTGCTGACCTCCGGGGCCGCACTG CCCTCCACCGTGGGGCAGTGACTGGCTGTGAGGACTGCCTGGCTGCACTGCTGGATCACGATGCATTTGT ACTGTGCCGAGACTTTAAGGGCCGCACACCCATTCACCTGGCCTCAGCCTGCGGCCATACTGCAGTGCTG CGGACTCTGCTGCAGGCTGCCCTTTCCACAGACCCCCTGGATGCCGGTGTCGACTACAGCGGATACTCGC CCATGCATTGGGCCTCCTACACTGGACATGAAGATTGTCTGGAGTTGTTACTTGAACACAGCCCGTTCTC ATACTTGGAGGGAAACCCCTTCACTCCTTTGCACTGTGCAGTAATTAATAACCAAGACAGCACCACAGAG ATGCTGCTGGGGGCTCTGGGTGCCAAGGTTGTGAACAGCCGGGACGCCAAAGGACGGACCCCCCTTCATG CCGCTGCCTTTGCGGACAATGTCTCTGGACTCCGGATGCTGTTGCAGCATCAAGCTGAAGTGAATGCCAC TGACCACACTGGCCGCACTGCGCTCATGACGGCCGCTGAGAACGGACAGACTGCTGCTGTGGAATTTCTG CTGTACCGAGGGAAGGCAGACCTGACTGTGCTGGATGAGAACAAGAACACTGCCCTTCACTTGGCTTGCA GCAAGGGCCATGAGAAATGTGCCCTCATGATCCTGGCGGAAACCCAAGACCTTGGCCTTATCAATGCTAC CAACAGTGCGCTGCAGATGCCGCTCCACATTGCTGCCCGGAATGGTCTGGCTTCTGTGGTGCAGGCCCTG CTGAGTCGTGGGGCCACAGTGCTGGCTGTGGATGAAGAAGGTCACACTCCAGCACTGGCCTGCGCTCCCA ACAAAGATGTGGCAGACTGCCTGGCCTTGATCCTCTCCACCATGAAGCCTTTCCCACCCAAGGACGCCGT CAGTCCTTTCAGCTTCAGCCTGCTCAAGAACTGCGGCATCGCAGCCGCCAAGACGGTGGGTGGCTGTGGC GCCCTGCCTCACGGGGCCTCCTGTCCCTACAGCCAGGAGCGGCATGGTGCCATTGGGTTAGATGGCTGCT ACTCAGAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001191875
Insert Size	3231 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191875.1, NP_001178804.1
RefSeq Size	3387 bp
RefSeq ORF	3231 bp
Locus ID	362811

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