Hdac4 (NM_053449) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217458 representing NM_053449 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCCAAAGCCATCCAGATGGACTTTCTGGCCGAGACCAGCCTGTGGAGCTGCTGAATCCTGCCC GCGTGAACCACATGCCCAGCACGGTGGACGTGGCTACAGCGCTGCCTCTGCAGGTGGCCCCTGCAGCAGT ACCCATGGACCTGCGCTTGGACCACCAGTTCTCACTGCCCTTGGAACCTGCACTGCGGGAGCAGCAACTG CAGCAGGAACTCCTAGCACTGAAGCAGAAGCAGCAGATCCAGCGGCAGATCCTCATTGCAGAGTTCCAGC GTCAGCATGAGCAGTTGTCCCGACAGCATGAGGCACAGCTGCATGAACATATCAAGCAGCAGCAGGAGAT GCTGGCCATGAAGCACCAGCAGGAGCTGCTGGAGCACCAGCGGAAACTGGAGCGGCACCGGCAAGAGCAG GAGCTGGAGAAGCAGCACCGTGAGCAGAAGCTGCAGCAGCTCAAGAACAAGGAGAAGGGCAAAGAGAGTG CTGTGGCGAGCACAGAGGTGAAGATGAAGCTGCAGGAGTTTGTTCTCAACAAGAAGAAGGCTCTGGCCCA CCGGAACCTGAACCACTGCATGTCCAGCGACCCCCGCTACTGGTATGGGAAGACACAGCACAGCTCCCTT GACCAGAGCTCTCCACCCCAGAGTGGGGTGTCAGCCTCCTACAACCACCCTGTCTTGGGAATGTACGACG CCAAAGATGACTTCCCTCTTAGGAAAACAGCTTCTGAACCTAACCTGAAATTACGGTCAAGGCTTAAGCA GAAAGTAGCTGAGAGACGGAGCAGCCCCCTGCTGCGCAGGAAAGATGGCCCTGTGGCCACTGCTCTAAAA AAGCGACCCCTGGATGTTACAGACTCCGCATGTAGCAGCGCCCCTGGCTCCGGTCCCAGCTCCCCCAATA GCAGCTCTGGCAACGTCAGCACCGAGAATGGCATTGCACCCACCGTGCCCAGCACTCCAGCTGAGACGAG CTTGGCACACAGACTTGTGACTCGAGAAGGCTCTGTTGCCCCACTTCCTCTCTATACATCACCATCCTTG CCCAACATCACCCTGGGACTTCCTGCCACTGGCCCTGCTGCTGGTGCAGCAGGCCAGCAGGATGCTGAGA GGCTTGCTCTCCCAGCCCTCCAGCAGCGAATCTCCTTGTTCCCTGGGACCCACCTCACCCCATACCTGAG CACCTCGCCCCTGGAGCGGGACGGTGGAGCAGCTCACAACCCCCTCCTGCAGCACATGGTCCTGCTGGAG CAGCCACCCACCCAGACGCCCCTTGTCACAGGCCTGGGGGCACTGCCCCTCCACACACAGTCCCTGGTTG GTGCGGACAGGGTGTCCCCATCCATTCACAAGCTGCGGCAGCACCGACCTCTGGGGCGCACGCAGTCAGC ACCCCTGCCGCAGAACGCACAGGCCCTGCAGCACCTGGTGATCCAGCAGCAGCACCAGCAGTTTCTGGAG AAGCACAAGCAACAGTTCCAGCAGCAGCAGCTGCACCTCAGCAAGATGATTTCTAAACCCAGTGAGCCAC CTCGGCAGCCTGAGAGCCACCCGGAGGAGACAGAGGAGGAGCTCCGTGAACACCAGGCCTTGCTGGATGA GCCCTACCTAGATCGGCTCCCTGGGCAGAAGGAGCCCTCCTTAGCTGGTGTGCAGGTGAAGCAGGAGCCC ATCGAGAGTGAGGAGGAAGAAGTAGAGGCCACTCGAGAGGCGGAACCCAGCCAGCGCCCAGCCACTGAAC AGGAGCTTCTCTTTAGACAGCAAGCTCTTCTGCTGGAGCAGCAGAGGATCCACCAGTTAAGGAACTACCA GGCATCTATGGAGGCTGCTGGCATCCCTGTGTCATTTGGCAGCCACAGACCTCTGTCTCGCGCACAGTCC TCCCCAGCATCTGCCACCTTCCCCATGTCAGTCCAGGAGCCCCCCACCAAACCAAGGTTCACCACAGGCC TTGTGTATGACACACTCATGTTGAAGCACCAGTGCACCTGTGGGAACACCAACAGCCACCCGGAGCATGC TGGGAGGATCCAGAGCATCTGGTCCCGCCTGCAGGAGACTGGCCTCCGTGGCAAGTGTGAGTGCATCCGC GGACGCAAGGCCACACTGGAAGAGCTGCAGACAGTGCACTCGGAGGCCCACACACTCCTCTATGGCACAA ACCCTCTCAACAGACAGAAACTGGACAGCTCGCTGACCTCCGTGTTTGTCAGGCTTCCTTGTGGTGGTGT TGGGGTGGATAGTGATACCATATGGAATGAGGTGCACTCGTCTGGGGCAGCCCGCCTGGCTGTAGGCTGT GTAGTGGAGCTGGTCTTCAAGGTGGCCACGGGAGAGCTAAAGAATGGCTTTGCTGTGGTTCGTCCCCCAG GACACCATGCCGAGGAGAGTACACCCATGGGTTTCTGCTACTTTAATTCTGTGGCAATTGCAGCCAAACT TCTCCAGCAGAGGTTGAATGTGAGCAAGATCCTCATTGTAGACTGGGATGTGCACCATGGGAATGGGACC CAGCAGGCCTTCTACAATGACCCCAATGTTCTCTACATGTCCCTGCACCGCTATGACGATGGGAACTTCT TCCCAGGAAGTGGAGCACCAGATGAGGTGGGCACAGGGCCAGGCGTGGGTTTCAATGTCAACATGGCCTT CACGGGTGGCCTCGATCCCCCCATGGGAGACGCCGAGTACCTGGCAGCCTTCAGAACGGTGGTCATGCCA ATCGCAAATGAGTTTGCCCCAGATGTGGTACTGGTGTCCTCGGGCTTCGATGCTGTGGAGGGCCACCCCA CACCTCTTGGAGGGTACAATCTCTCTGCCAAATGTTTCGGGTACTTGACAAAACAGCTGATGGGCTTAGC TGGTGGCCGGATCGTGCTGGCCCTGGAGGGAGGCCATGACCTGACAGCCATCTGTGATGCTTCTGAAGCA TGTGTTTCTGCTCTGCTGGGAAATGAGCTTGAGCCTCTGCCAGAAAAGGTTCTACATCAGAGACCCAATG CCAATGCCGTCCACTCCATGGAGAAAGTGATGGGCATCCACAGTGAGTACTGGCGCTGCCTGCAGCGTTT GTCCCCCACTGTGGGGCACTCTCTGATTGAGGCGCAGAAGTGTGAGAATGAAGAAGCTGAGACAGTCACC GCCATGGCCTCGCTGTCTGTAGGCGTCAAACCTGCTGAGAAGAGATCTGAGGAGGAGCCCATGGAGGAGG AGCCACCACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053449
Insert Size	3234 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053449.1, NP_445901.1
RefSeq Size	4015 bp
RefSeq ORF	3234 bp
Locus ID	363287
UniProt ID	Q99P99
Gene Summary	human homolog is a component of the DNA damage response pathway and is also involved in repression of muscle differentiation [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.