Slc12a7 (NM_001013144) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Kcc4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217462 representing NM_001013144 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACGAACTTTACGGTGGTGCCGGTGGAGGCGCGCGCCGACGGCGCCGGGGACGAAGCTGCTGAGC GCACGGAAGAACCCGGGTCTCCCGAGAGCGCGGATCCTGCCTGCCCTACGCCGGGAGATGGAAACCCCAG GGAGAACAGCCCATTCATCAATAATGTGGAGGTGGAAAGAGAGAGCTACTTCGAGGGGAAGAACATGGCG CTTTTTGAGGAAGAGATGGACAGCAACCCCATGGTATCATCACTGCTGAACAAGCTGGCCAACTATACCA ACCTGAGCCAGGGTGTGGTAGAGCATGAGGAAGATGAGGACAGCCGGAGGCGGGAGATCAAGGCCCCACG CATGGGCACCTTCATCGGAGTCTACCTACCGTGCCTGCAGAACATCTTGGGTGTAATCCTTTTCCTGCGC CTGACCTGGATTGTGGGGGCGGCTGGCGTTCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACCTGTA CAATGCTGACAGCCATCTCCATGAGTGCCATCGCTACCAACGGTGTGGTCCCAGCGGGAGGCTCATACTA CATGATCTCCCGTTCGCTGGGGCCTGAGTTTGGAGGGGCTGTTGGCCTCTGCTTCTACTTGGGCACAACG TTCGCAGGCGCCATGTACATCCTGGGAACCATCGAGATCTTCCTGACCTACATCTCTCCAAGTGCAGCCA TCTTCCAGGCAGAGACAGCAGACGGCGAGGCGGCCGCGCTGCTGAACAACATGCGTGTGTATGGCAGCTG CGCACTGGCGCTCATGGCCGTGGTGGTCTTCGTCGGAGTCAAATATGTCAACAAGCTGGCCCTGGTCTTC TTAGCCTGTGTTGTGCTTTCTATCCTGGCCATCTATGCTGGTGTCATCAAGACAGCCTTCGCCCCACCTG ACATCCCGGTCTGCCTTCTAGGGAACCGCACGCTGGCGAATCGCAACTTTGATACCTGTGCCAAAATGCA GGTTGTCAGCAATGGCACAGTCACCACCGCGCTCTGGCGCCTCTTCTGCAACGGCTCCAGCTTGGGTGCC TCCTGCGATGAGTACTTTGTGCAGAACAATGTCACTGAGATCCAGGGCATTCCTGGTGTGGCCAGTGGTG TCTTCCTGGATAACCTGTGGAGCACGTATTCGGACAAGGGGGCATTTGTGGAAAAGAAGGGCGTGTCCTC GGTGCCTGTGTCCGAGGAGAGCCGGCCTGGTGGATTGCCATATGTCCTCACAGACATCATGACCTACTTC ACCATGCTAGTCGGCATCTACTTCCCCTCCGTAACGGGGATCATGGCAGGATCCAACCGCTCCGGGGACC TCAAAGATGCCCAGAAGTCCATCCCAACAGGGACCATTCTGGCCATTGTGACTACATCTTTCATTTATCT TTCCTGCATTGTGCTGTTTGGGGCCTGCATCGAGGGTGTAGTCTTGCGAGATAAGTTTGGGGAGGCCCTG CAAGGGAACTTGGTCATTGGCATGCTGGCTTGGCCATCTCCCTGGGTCATCGTGATTGGCTCCTTCTTCT CCACCTGTGGTGCTGGCCTGCAGAGCCTAACTGGGGCACCCCGCCTGCTGCAGGCCATTGCACGCGACGG CATCATCCCCTTCCTGCAGGTGTTTGGCCATGGAAAGGCCAACGGGGAGCCCACGTGGGCCCTGCTGCTC ACGGCCCTCATCTGTGAGACCGGCATCCTCATCGCCTCCCTGGACAGCGTGGCTCCCATCCTGTCCATGT TCTTCCTCATGTGCTACATGTTCGTCAACCTGGCCTGTGCTGTTCAGACCCTGCTGCGCACACCCAACTG GCGTCCACGTTTCAAGTTCTACCACTGGACCCTCTCCTTCCTTGGGATGAGTCTCTGCCTTGCGCTCATG TTCATCTGCTCCTGGTACTACGCCCTCTTCGCCATGCTCATCGCGGGCTGCATCTACAAGTACATCGAGT ACCGAGGGGCTGAGAAGGAGTGGGGGGATGGCATCAGGGGCCTGTCACTGAATGCTGCCCGCTATGCCCT GCTGCGTGTGGAACATGGGCCCCCACATACCAAGAACTGGAGGCCCCAGGTATTGGTGATGCTGAACCTG GACTCTGAGCAGTGTGTAAAGCACCCCCGCCTGCTGTCCTTCACCTCTCAGCTGAAGGCTGGCAAGGGCC TGACCATCGTGGGATCTGTGCTGGAGGGCACCTACTTAGACAAGCATGTGGAGGCCCAGCGGGCTGAGGA GAATATCCGGTCTCTGATGAGTGCAGAGAAGATGAAAGGCTTCTGCCAGCTGGTGGTGTCGTCCAACCTG CGAGACGGTGCATCCCACTTGATCCAGTCAGCTGGCCTTGGTGGCATGAAGCACAACACTGTCCTCATGG CCTGGCCAGAGGCTTGGAAGCAGGCAGATAACCCTTTCTCCTGGAAGAACTTTGTGGACACTGTCCGTGA CACTACAGCAGCACATCAGGCACTGTTGGTGGCCAAGAACATCGACTTGTTCCCGCAGAACCAGGAGCGC TTCAGCGACGGCAACATCGATGTGTGGTGGATCGTGCACGATGGGGGGATGCTCATGCTTCTGCCGTTCC TGCTGCGCCAGCACAAGGTGTGGCGAAAGTGCCGGATGCGCATCTTCACGGTCGCCCAGGTGGACGATAA CAGCATCCAGATGAAGAAGGACCTGCAGATGTTCCTGTACCATCTCAGGATCAGCGCCGAGGTGGAGGTG GTAGAGATGGTTGAAAATGACATTTCTGCATTCACCTATGAGAAGACGCTAATGATGGAGCAGAGGTCAC AGATGCTGAAACAGATGCAGTTGTCAAAGAATGAGCGGGAGAGAGAGGCCCAGCTGATTCACGACAGGAA CACTGCATCCCACACCGTAGCAACGGCTAGAACTGAAGCCCCACCAACACCTGACAAAGTGCAGATGACA TGGACGAAAGAGAAACTCATTGCAGAGAAACACAGGAACAAGGACACCGGCACATCAGGCTTCAAAGACC TTTTCAGCCTAAAGCCGGACCAGTCCAACGTCAGAAGGATGCATACTGCTGTGAAGCTCAATGGTGTGGT TCTCAACAAGTCCCAGGATGCTCAACTCGTCCTGCTGAATATGCCAGGCCCCCCAAAAAGTCGGCAGGGC GACGAGAACTACATGGAATTTCTTGAGGTCTTGACCGAAGGGCTGAACAGGGTCCTCCTGGTCAGGGGTG GTGGCCGGGAAGTCATCACCATCTACTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001013144
Insert Size	3252 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001013144.2, NP_001013162.2
RefSeq Size	5003 bp
RefSeq ORF	3252 bp
Locus ID	308069
UniProt ID	Q5RK27
Gene Summary	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).[UniProtKB/Swiss-Prot Function]

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