Aff3 (NM_001191887) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Laf4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217552 representing NM_001191887 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACTCTAGGCCGTGTCTATGAACCAGATAGGAATGCGTTACGGAGGAAAGAACGGGAAAGGAGAA GTCAGGAAACGCAGCAGGACAGCGGCGGCTTTAACTCCGGTTACTCACTCTTCAGTGAACCCTACAAGAC TAACAAGGGGGATGAACTCTCCAACCGGATCCAGAATACATTAGGAAATTATGACGAGATGAAAGACTTT TTAACTGACCGATCCAACCAGAGTCATCTTGTTGGCGTCCCCAAGCCTGGTGTTCCCCAGACTCCTGTGA ACAAGATCGATGACCATTTTGTGGCAGAGTCAAGAGCCCAGCCCCAGCCCTCATCTGTCTGCAGCACTGC ATCTTCCACACCTGCAGCTGTCCCAGTGCAGCAGAGCAAGAGAGGCACCATGGGCTGGCAGAAGGCTGGG CACCCACCGTCTGACGGCCAACAGAGAGCAGCGCAACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTG TTGGCAGACAGCAGACAAGGGCCAAGCAAGTGTGCAATGTGGAGACGGGGCTTCAGACACAGGAGAGGCC GCCTGCAATGGCAGCCAAGCACAGTGGCGGTGGACACTGCGTTCAGAACTTTCCCCCATCCCTGGCTTCA AAACCCAGCCTGGGGCAGCAGAAACCAACCGCCTATGTGCGGCCAATGGATGGCCAGGACCAGGCTCCCG ACGAATCTCCTAAGCTTAAGTCATCCACGGAAACTGCTGTGCACTGCACGGCCTACAGAGGGGTCCCGGC CGGCAAACCAGAGTCAGCCAGAGCCAAGGGCAAGCTCGCTAAGTTCAGCATCCCCAAGCAAGGCGAGGAG AATAGATCTGGAGAAACCAACAGCTGTGTGGAAGAAATAATTCGGGAGATGACCTGGCTTCCTCCACTTT CCGCCATTCAAGCTCCTGCCAAGGTGGAACCAAGCAAGTTCCCATTCCCAAATAAGGACTCACAACTTGT ATCCTCTGGACACAGTAATCCAAAGAAAGCTGATGCGGAGCCAGAGAGTCCAGACAATGGCACGTCGAAC ACTTCAACTCTGGAAGACGACCTTAAGCTAAGCAGTGATGACGAAGAGGGTGAGCAGGCAGCCCAGAGAA CGGTTCTCCGCGCTCTAGCTGACAGCTCCGTGGTCCAGCAGACCAACTGTAGAGGTTCCGCGCCATCCAG CAAGGGCAGCAGCAGCAGCAGCGGCGGCAGCAGCTCCTCCAGCGACTCAGAAAGCACTTCCGGCTCCGAC TCCGAGACGGAGAGCAGCTCCAGCGAGAGCGAGAGTGAGGGCAGCAAGCCTCCGCACTGCTCCAGCCCCG AGGCCGAACCTGCGTCCTCGAACAAGTGGCAGTTGGATAAATGGCTGAACAAAGTTAATCCTCACAAGCC GCCAATTCTGATCCAAAATGAAAGCCATGGCCCGGAAAGGAATCAATACTACACGCCTGTGAAAGATGAA GGGCAAGACTGCGGGAAACTTCCTGAGATCTGCCAAGCCAGCCTGAGGGACAAGGAGCTCAAGACTGCCT GCAAGGAGGAGCAGCGGCCGAGGACCGCAAACAAGGTCCCTGGCAGCAAAAGTGTCAAGCAAAAGTCCCC GCCCGCTGCGGTGGCTGTGACCGCAGCTGCCCCGCCGCCCGCGGTGCCCAGCGCACCCACGGAGAGTGCA CCCGCGCCTACCCGCAGGTCAGCAGGCAAGAAGCCCACCAGGCGCACCGAGAGGACCTCAGCCGGGGACA GTGCCAACTGCCACCGACCAGAGGAGCCAGTGGCCCCAGACGCGCTGGGGGCGAGCGTAGTGGGACCCCT AGAGCCCCCCAAAACCAGGCCCTGTGGTAACAGCAGGACAGGCCACCGCAAGGAGCTGCGCTCCTCAGTG ACCTGTGAGAAGCGTCGCACGCGGGGGCTGAGCAGAATTGTCCCTAAATCCAAGGAGTTTATTGAAACAG AATCTTCGTCTTCCTCTTCCTCCTCGGACTCAGACCTGGAGTCAGAGCAAGAGGAGTACATCCTGTCCAA GGCTCCAAGCACCACCGGGAGTGAACAGAGGCTAAAGGAGGCCACCAGCAACAACAGCAGCAGCAGCAAC AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCCCGAGCCTCTGTGGGCTCCA TCAACGCCAGGACCACCAGTGACATTGCCAAGGAGCTAGAGGAACAGTTCTACACGCTGGTCCCCTTTGG CAGGAATGAACTGCTTTCTCCACTAAAGGACAGTGATGAGGTCAGGTCTCTCTGGGTCAAAATTGACCTG ACACTCCTTTCCAGGATCCCAGAACACCTGTCCCAGGAGCCTGGGGTCTTGAGCGCTCCTGCAGCCAAGG ACACTGACAGTACCCCAGCCAGCCATGCATTGGACACACCTGCTGAAAAGACTTTGCCAAAATCCAAGAG GAAACGCAAGTGTGACAATGAAGACGACTACAGGGAGATAAAGAAGGCCCAGGGAAAGAAGGAGGGTGCC TCACGCCTGGCTGCCTCCACTAACAACACCTTCTCCGGCAGCCACTGCAACGTGAGCATCAACAGCTTGG CAATACCAATAAACAAAAATGAAAAGATGCTCCGGTCACCCAGCTCGCCACTCTCTGATGCATGTAAACA CAAATACTCCAGCGAGGACGTGACTTCTTCCAGCCGACCTCACGGCAACGGGTTGTTGACTTCTGTGTCT TCCAACAAAGAGCCTAAGGCCGAGAGCCAGCTGCAGTCGCATGCTGGGGACCTCACGAAGATCTCTCACA ACAGTTCCGAAAATGGCATCCTCCACAGCAAATCACGGCCCCAGACTGAGCCATGGTCTCCAGGTTCCAA CGGCCACAGGGACTGCAAGAGACAGAAACTGATCTTCGATGACATGCCCCGAAGTGCGGATTATTTCATG CGAGAAGCTAAGCGGATGAAACACAAAGCAGACGCCATGGTGGAGAAGTTTGGGAAGGCCCTCAACTATG CCGAAGCAGCCCTGTCCTTCATCGAGTGTGGAAACGCAATGGAACAAGGGCCGATGGAGTCCAAGTCTCC GTACACCATGTACTCGGAGACAGTGGAGCTCATCAGGTATGCTATGAGACTAAAAACCCACTCAGGCCCC AATGCCACACCGGAGGACAAACAGTTAGCTGCGCTTTGTTACCGATGCCTGGCTCTCCTATACTGGCGGA TGTTTCGACTCAAAAGGGACCATGCTGTCAAGTATTCAAAAGCACTCATCGACTATTTCAAGAATTCATC GAAAGCTGCCCAAGCCCCATCCCCATGGGGGTCTAGCGGCAAGAGCACTGGAAGCCCATCCCCCATGTCT CCCAACCCTTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTTTCCAGTTCCAGCGGCCTGTCCCCAT CGACCATTGTGAGCATCCCCCAGCGAATTCACCAGATGGCGGCCAATCACGTCAGCATCACCAACAGCAT CCTCCACAGCTACGACTACTGGGAAATGGCTGATAACCTGGCCAAGGAAAACAGAGAGTTCTTCAATGAC CTGGATTTGCTCATGGGACCTGTCACCCTGCACAGCAGCATGGAGCACCTGGTCCAGTACTCCCAGCAAG GCCTGCACTGGTTACGGAGCAGCACACACCTGTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191887
Insert Size	3678 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191887.1, NP_001178816.1
RefSeq Size	3806 bp
RefSeq ORF	3675 bp
Locus ID	363220

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