Mdc1 (NM_001166275) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217573 representing NM_001166275 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACACTCAGGTGATTGACTGGGATGCTGAAGAAGAGGAAGAGACAGAAATATCCAGTGGATCCT TGGGGTATAGTGTGGAGCCTATAGGGCGGCTGCGATTCTTCAGTGGTACCCATGGACCAGAACGAGATTT CCCACTCTACCTTGGCAAGAATGTAGTTGGTCGAAGCCCTGACTGCTCTGTGGCCCTGCCTTTTCCATCC ATCTCCAAACAGCACGCAGTGATTGAAATCTCAGCTTGGAACAAAGCCCCTATCCTTCAGGATTGTGGGA GCCTCAATGGCACTCAAATCGTAAAGCCTCCTAGGGTCCTAGCTCCTGGAGTGAGCCATCGTCTGAGGGA CCAGGAATTAATTCTGTTTGCAGACTTTCCCTGCCAGTACCATCGCCTGAATGTCCCACCACCCTTGGTC CCTCGGAGTCTTCTAACTATAGAGAAGACCCCCAGAATACGGGGAAGATCCCAAAATTCCAGAGTTTTGT TGGCTGAGGATTCAGAGGAAGAAGGAGATTTTCCTTCTGGAAGGTCTGTGGCAAATGGATCAAGGAATAC AGCATCCCCTTCAGCAACAGTAGTTCCAGAAAGTGATGAAGAGGGCTCTTCTCCAGGCCCAAGTGTCCCT GGGCCATCTTCACCCTTTGGTTTGGGAAGTGACACAGATGAATCACAAGGTCAGCAACCAGGAGTAGAGG AATCTTCTTTAGCTGACAACAGTGGTGCCGCAGGGGAGCCTGAGCAGCCTGAAGTGAATGGAGTGACAAC TGGCACACTGGCTCAGCCTACCAAGGACAAGTTCAAGGACACAAAGATGAAGGAGGAGGCAGGCAGTGCA GGGGTTCCAGTTGGGTCAGTTGTGGAGGGGAGCCCCACTCTTGGGGAAGACAGTGACACAGAAGCGGATG AAGAGCGTCAGCCTTCTGGTTCTGGGGATAGTGATACGGACGTAGAAGAAGAGAGGGTCCCTGTGAAGAA GAACCAAGTACTGCTTGGGGTTGGTATAGGGGGTCCAGGAGCACGTGGTGTGGCACATCTGCAGGACAGC CCAACTGGTAGTGACACAGATGTGGAGGAGGACAAGACTGCACTGGCTGCCCCTCCAGAGAGAAGCCACA CAGCCATGGTGATCAACAGTGATACAGACGAGGAGGAGAGGGGTGAGGAGGAAGAGGTCTCGGCAGCACT CACCTTGGCCCGTCTGAAGGAGAGAGGAATTGCTTTGTGGAGTGGAGAGCCAGGCACAGAGGAGGTCAAG TCCCAACCACAGGTCCTTGTAGAACGAAGCCAGAGTGCCTCTGGGAGAGACAGTGACACTGACGTGGAGG AGGGATCCTCAGGGGGAAAGAGAGAGATTGTCCCTGACAGCCCCATGGATGTAGATGAGACTCTTACTGT CACACAGCCAGAGAGCCAGCCTCCCTGTAGGCCCAATGATGTAGACGAGGATGTGGATATGAGCTCCCCT GGCAGCCATCTAGAGGGAAAGAAGGCCTCCTCTGCTCTGGTAGACAAGAACAGAGCACAAGTGGAGGAGG AAGTCCCAGGGCCATCTGTTACCCTGGGGGAGAAACACCAGGTGCCTCTAGAGGGAGCCCAGCCTCCTGA GGAAGCCCGGGAAACAGCTGTGCAGGAAGGCTCATCCTCACCAGTGGCAGACATAAGAATGAGCCAGCAG CCAGTAGCGGAAGATGCTGGGACAGAGTGTGCTGCAGCTGTTTCTGAACAGAAAAGTGCTCTTGAGGTCG GGGCTCAGAGCAGGTCACCTGCAGCACCCGTGGAGCAGGTGGTGGTACGCACAGATACTTCAGGGGATCC TACCCTGCCACAGAGAGAGGGAGCCCAGACCCCCACAGGAAGGGAAAGAGAAGCACATGTGGGCGGGACC AAGCATGCCAAAGAGTGCTGTGATGAACCTGAGGATCTGTGCCTTTCAGCCACCCAGTGCTTTGTGGAAG GGGAGAGCCAGCACCCAGGAGCTGTCCAGAGTTTGGAAGATGAGCCTACCCAAGTCTTCCCATGCCTTCC CCAAGAGCCTGGACCTTCCCATCTCAGCCTCCCGACTCCAGGTGCAGATACCTTGGATGTGCCTTGGGAA GTCTTGGCTACACAGCCGTTCTGTCTGAGAGAACAGACAGAGACCTCTGAGCCCATTGACACCCATGAAG CTCATGGATCTCAACCATCTCTACCTGGGGAACCACCAGGACACCAACATCCGGTCCCTACCAGCCTAGA TCACACAGAGCTGTTGAGAATTGATGACAGAGAGATGCAGACTGTGGAGAAAGCCATGGGCCACCTAAGT TGCCAGATGATGCCAGATGGAAAGGCTTCCGGGGATGACCCAGAGCCCTCAGACCATCGCCTGTTTTCTC CAGTGCCTGAAGCTTCAGCTTCACCTCAGAGTCTCCTCACCTCTCAGAGCCAAAAGCAATCTACACCTCA GCCTATGTTTCCCACTTCTTCTTCTGAGCTAGCCCTTCCTGAGACTCTTCACACAAAGCCTAATGTCAGG CCACGGCGGTCCTCCAGGATGACCCCCTCCCCACATTCCTCTGCTGCCCTTAAGCCTTATACTACCTGCC CCACAAACCAGCCTGCTGCTTCTAGGCCAACATCTCGCCCCACTCGGGGCAGGGCAAATAGGTCCTCTAC CAGGACCCCAGAACTGATTGTCCCTACAGGCCCTGAGCTCCAGCCTTCCACCTCCACAGAACAGCCTGGC ATTCCAAACCTCACTTCTCAGGTCACTGAGGGCAGGGCACATAGTACCTCTGTTAATATGCCTGAACCAG TTCTCACAGGTCCCGAAGCCCAGCCTCTCACCTCCGCAGAACAATCTGTCACCTCAAACCTCAATCCTCG GGCCCAGCCTCTTACCCTGGAGCCAGTTCCACAAACCAGTCACCAAAGGAGGCGAAGGGCTACTGGGAAG CAGGGCTCCCGCACAGCTCCTGTTGGTCCTAAGTCTTACTCTACCCCCGCTGAACCTGAACCCCAGTCTT CAGCCAGCCAAAGCTCAGGAGCATCAGAAGCAGATTCACCCCATCAAAAACGTCCCCGAAGACAAGTCAC CCAGAAAACTGTAGTCGTCAAGGAAGAAGATCCTGGAGAAATACAAGTGAAGGAAGAGCCTCAGGAGACA GCAATTCCAACACCAGGCAAGAGAAAGAGGGACCCTGCAGAGGGGGAAACCCAGGGAAACCCAACTCGAA GCCGGCGAACTAAGCCTAACCAGGAAGCAGCAGCCCCCAAGGTACTGTTCACAGGAGTGGTGGATTCTCG TGGAGAGCGTGCAGTGCTGGCTCTAGGAGGCAGTCTGGCCAGCTCAGTAAATGAGGCCTCCCACTTGGTT ACTGATCGCATCCGCAGGACAGTCAAGTTCTTGTGTGCCGTGGGGAAGGGAATTCCCATCCTGTCCCTGA ACTGGCTCTATCAGTCCCGAAAGGCTGGTTGCTTCTTGCCGCCTGATGACTACTTGGTGACTGATCCTGA ACAAGAGAAGAATTTTAGCTTCAGCCTTCGGGATTCCCTGAGCCGGGCTCGGGAACGAAGACTACTGGAG GACTATGAGATTCATGTGACCCCTGGAGTGCAGCCACCTCCACCTCAGATGGGAGAGATCATCAGCTGCT GTGGAGGCACTGTCCTGCCCAGCATGCCCCATTCCTACAAGCTTCACAGAGTTGTCATAACGTGCACTGA AGACCTACCTCGATGTGCTATTGCATCTCGCCTGGGGCTACCCCTCCTCTCTCCTGAGTTCCTCCTGACT GGAGTGCTGAAGCAGGAAGCCACACCAGAGGCCTTTGTCCTCTCCAATTTGGAAATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001166275
Insert Size	3840 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001166275.1, NP_001159747.1
RefSeq Size	4072 bp
RefSeq ORF	3840 bp
Locus ID	309595
UniProt ID	Q5U2M8
Gene Summary	Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (By similarity).[UniProtKB/Swiss-Prot Function]

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