Sympk (NM_001100830) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hfn3g
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217574 representing NM_001100830 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCGGCAGCGGGGACAGTGTCACCCGCCGGAGTGTGGCATCACAGTTTTTCACACAAGAAGAAG GGCCAGGCATTGATGGCATGACCACTTCTGAAAGGGTGGTAGATCTCCTGAACCAGGCTGCGCTGATCAC CAATGACTCAAAGATCACAGTGCTCAAACAGGTCCAAGAGCTGATCATCAACAAGGACCCCACCCTGCTT GACAACTTCCTGGACGAGATCATCGCTTTCCAAGCAGACAAGTCCACTGAAGTGCGCAAGTTTGTCATTG GCTTCATCGAGGAGGCATGCAAGCGGGACATTGAGCTGCTGCTAAAACTGATCGCCAACCTCAACATGCT ATTGCGGGATGAAAACGTGAATGTGGTAAAGAAGGCCATCCTCACCATGACCCAGCTCTACAAGGTGGCC CTGCAGTGGATGGTGAAGTCTCGGGTCATCAGCGACCTCCAGGAGGCCTGCTGGGACATGGTGTCCTCCA TGGCTGCAGAAATCATACTGCTCTTGGATTCTGACAATGATGGCATCCGTACACATGCCATTAAGTTTGT GGAGGGCCTCATTGTCACTCTGTCACCCCGAATGGCTGACTCAGAGGTCCCCCGACGCCAGGAGCATGAC ATCAGTCTGGACCGCATCCCTCGAGATCACCCCTACATCCAGTACAATGTCCTATGGGAAGAAGGCAAGG CAGCTCTGGAGCAGCTTCTCAAGTTCATGGTGCACCCCGCCATCTCCTCCATCAACCTGACCACAGCGCT CGGCTCCCTTGCCAACATCGCCCGCCAGAGACCCATGTTCATGTCTGAGGTGATCCAGGCCTATGAGACT CTGCATGCCAACCTGCCCCCAACACTGGCCAAGTCCCAGGTGAGCAGCGTGCGTAAGAACCTCAAGCTGC ACTTGCTGAGCGTGCTCAAGCACCCTGCCTCCTTGGAGTTCCAGGCCCAGATCACCACCCTGCTGGTGGA CCTGGGCACGCCCCAGGCCGAGATTGCCCGTAACATGCCTAGCAGCAAGGACTCCCGCAAGCGGCCCCGG GACGATACAGAATCCACGCTCAAGAAGATGAAGCTGGAGCCCAACCTGGGAGAAGATGATGAGGACAAAG ACTTGGAGCCTGGCCCATCGGGGACATCAAAGGCCTCAGCCCAGATCTCAGGCCAGTCAGACACAGACAT CACTGCTGAGTTCTTGCAGCCTCTGCTGACACCTGACAATGTGGCCAATCTGGTCCTCATCAGCATGGTG TACCTGCCCGAGACCATGCCTGCCTCCTTCCAAGCCATTTACACCCCCGTGGAGTCAGCAGGCACAGAAG CCCAGATCAAGCACCTGGCACGGCTCATGGCCACACAGATGACAGCTGCGGGACTGGGTCCTGGTGTGGA ACAGACGAAACAGTGCAAGGAGGAGCCCAAGGAGGAGAAGGTGGTAAAGCCGGAGAGCGTCCTGATCAAA CGGCGCCTGTCAGTGCAGGGCCAGGCCATCTCTGTGGTGGGCTCTCAGAGCACCATGTCCCCCTTGGAGG AGGAAGTACCCCAGGCCAAGAGGAGGCCAGAACCCATCATTCCTGTCACACAGCCACGGCTGGCAGGCGC TGGTGGGCGCAAGAAGATCTTCCGTCTGAGCGATGTGCTGAAGCCCCTGACAGATGCCCAGGTGGAAGCC ATGAAGCTGGGAGCTGTGAAGCGGATCCTGAGGGCTGAGAAGGCTGTAGCCTGCAGTGGGGCGGCCCAGG TGCGCATCAAGATCCTGGCCAGCCTCGTGACACAGTTCGACTCCGGATTCAAGGCAGAGGTGCTGTCCTT TATCCTGGAAGACGTCCGGGCCCGTCTGGACCTGGCTTTTGCCTGGCTCTACCAGGAGTACAATGCCTAC CTGGCAGCGGGGCCCTCTGGCACTCTGGACAAGTATGAAGACTGCCTTATCTGCCTGCTCTCTGGACTTC AAGAGAAACCAGACCAGAAAGATGGGATCTTTACCAAGGTGGTTTTGGAGGCCCCGCTGATCACTGAGAG TGCTTTGGAGGTGATACGGAAGTACTGTGAGGATGAGAGTCGCGCCTACCTGGGCATGTCGACACTTGGA GACCTGATCTTCAAGCGCCCTTCTCGCCAATTCCAGTATCTGCACGTTCTCCTCGACCTCAGCTCTCACG AGAAGGACAGGGTGCGCTCCCAGGCCCTGCTCTTCATTAAGCGCATGTATGAGAAGGAGCAGCTTCGAGA ATATGTGGAGAAATTTGCCCTCAACTACCTGCAGCTCCTGGTCCACCCCAACCCACCCTCAGTGCTGTTT GGAGCTGACAAGGACACAGAGGTGGCTGCGCCCTGGACAGAGGAAACAGTGAAGCAGTGTTTGTACCTAT ATCTGGCTCTCCTGCCTCAGAACCACAAGCTGATCCATGAGCTGGCAGCAGTGTACACGGAGGCCATCGC CGACATCAAGCGGACAGTGCTGAGAGTCATTGAGCAACCGATCCGAGGGATGGGCATGAACTCACCAGAG TTGCTGCTGCTGGTGGAAAACTGTCCCAAGGGGGCAGAGACGCTGGTCACCCGCTGCCTGCACAGCCTCA CTGACAAAGTGCCACCATCCCCAGAACTGGTGAAGCGGGTCCGGGACCTGTACCACAAGCGGCTGCCTGA CGTGCGCTTCCTCATCCCTGTGCTCAACGGCTTGGAGAAGAAAGAGGTGATCCAGGCCCTGCCTAAGCTC ATCAAACTCAACCCCATTGTGGTGAAGGAGGTCTTCAACCGCCTGCTAGGCACCCAGCATGGGCCCTGTG TGGTAGAGGTAGAGAAGCTTGGGGAGTGGGGTTCCCACTTAATGAGGGACATCCAGGCAGAGAGAGGACA GGCCACTCTCCGCCTCCTCACAGCCACCAACCTGTGTTTTGCGGAGCGGAATGTGTACACATCGGAAGTG CTGGCTGTGGTGATGCAGCAGCTGATGGAGCAAAGCCCTCTGCCCATGCTGCTCATGCGGACCGTCATCC AGTCCCTGACCATGTACCCCCGCCTGGGGGGCTTCGTCATGAACATCCTGGCCCGGCTCATCATGAAGCA GGTATGGAAGTACCCCAAGGTATGGGAGGGCTTCATCAAGTGCTGCCAGCGCACCAAGCCCCAGAGTTTC CAGGTCATCCTGCAGCTACCTCCACAGCAGCTTGGCGCAGTCTTTGACAAGTGCCCTGAGCTCCGGGAGC CACTGTTGGCCCATGTGCGCTCCTTCACCCCCCACCAGCAAGCACACATCCCCAACTCCATCATGACAAT CCTGGAGGCCAGTGGCAAGCAGGAGCCGGAAGTCAAGGAAGCACCTTCAGGGTCCCTGGAAGAGGATGAC CTGGAGCCCTTGGCTCTGGCCTTGGCCCCGGCTTTGGCCCCGGCCCCTGCCCCACGGCCCCCTCAGGACC TCATCGGCCTTCGATTGGCTCAGGAGAAGGCCTTGAAGCGGCAGCTCGAAGAAGAACAGAAGCAGAAGCC CACAGGCGTCGGAGCGCCCACTTCCTCAGTGTCCTCGACACCCTTGGTGGGGCCAGCAGCCCGAGCTGGT CCCACCCCGGCTGAGGAGGTTATGGAATACCGGGAGGAAGGTCCTGAATGCGAGACCCCGGCCATCTTCA TCAGCATGGACGACGACTCAGGGCTGGCCGAAACCACACTTCTGGACTCTAGTCTCGAGGGACCCCTGCC TAAGGAGGCAGCAGCAGTCGGACCGAGCTCCAAGGACGAGCGGAGCCCCCAGAACCTCAGCCATGCTGTG GAAGAAGCCTTGAAGACCTCCAGCCCAGAGGCCAGGGAACCCGAGAGCAAGGGGAACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100830
Insert Size	3843 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100830.1, NP_001094300.1
RefSeq Size	4031 bp
RefSeq ORF	3843 bp
Locus ID	292683

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