Ttc21b (NM_001191737) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1565122
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217588 representing NM_001191737 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCCAGGGGCTGAAGACACTGATCAATTACTATTGTCAAGAGAGATACTATCACCATGTGCTCC TTGTGGCCAGTGAAGGGATGAAGAAGTATAGCAGTGACCCAGTCTTCCGATTTTACCATGCCTACGGCAC ACTAATGGAGGGTAAAGCTCAAGAAGCCCTTCGGGAGTTTGAGGCCATTAAAAATAAACAAGACGTGTCA CTGTGTTCCCTGATGGCGCTGATGTATGTCCATAAAATGAGCCCCAATCCAGATAGAGAAGCTATCCTGG AATTAGATACCAAAATGAAGGAGCAACGTAAGGAAGCCGGACGCAAAGCCTTGTACCATGCAGGCCTGTT TCTGTGGCACATTGGTCGCCATGATAAGGCGAGAGAATATATTGACAGAATGTCAAAAATGCCACATGAT AGCAATGAGGGACCGATTTTGAAAGCATGGCTTGATATTACAAGAGGGAAGGAACCTTATGCTAAAAAAG CCCTACGGTATTTTGAGGAGGGATTACAAGATGGAAACGATATTTTTGCTCTGCTGGGTAAGGTACTGTG CCTTGAGATGCGACAGAATTATTCTGGAGCTCTGGAGACTGTGAGTCAGATAATTGTGAACTTCCCGAGC TTCCTTCCTGCTTTTGAGAAGAAAATGAAATTACAACTGGCTTTGCAGGATTGGGATCAGACGGTGGAGA CGGCGCAAAGGTTGCTGCTTCAGGACGGCCACAATGTGGAGGCGCTGAGAATGCTGGCTTTATATTATCT GTGTAGGGAAGGGGACATAGAGAAGGCTGCTGCCAAGCTGGAAAATTTAGGAAATGCATTGGATGTCATG GAACCACAGAATGCTCAACTTTTTTATAAGATTACAATAGCCTTCAGCAGAACTTGTGGACGTAATCAAC TCATTCTTCAAAAAGTTCAAAGTTTCCTAGAAAAAGCATTTAGTTTAACCCCCCAGCAAGCAGAAATTGC TACAGAGCTTGGCTACCAAATGATTCTCCAAGGCAAAGTCAAGGAGGCCTGGAAGTGGTACAGGACCGCC ATGACACTGAACGAAAGCAACATCTCTGCCGTCACCGGACTTATCCGATGTCAGTTAATAGAAGGACAAT TGCAAGATGCAGACCAGCAGTTGGAGTTCTTCAGTGAATTCCAGCAGTCAATGGGAAGATCTGCGGAATT AATGTATTTGCATGCGGTTCTTGCTACCAAAAAAAATAATCGTCAGGAGGAAGTTATAAATTTGTTGAAT GATGTTGTGAACACTCACTTTTCACACTTGGAAGACCTCCCTCTCGGCATACAGTATTTTGAGAAGCTCA ACCCTGACTTCTTACTAGAAGTTGTTAACGAGTATCTGAATCTCTGTCCAATTCAGCCTGCAGGTCCTGG GCAGCCTCTCTCCCCAGTCCTCAGACGTTGTTCTTCAGTTCTGGAGACGATTATAAGAAGTGTACCAGGT CTTCCGCAAGCTGTCTTCCTAATGGCCAAAGTGAAGTATCTGTCAGGTGACACCGAGGCAGCCTACAACA ACCTGCAGCACTGCCTGGAGCACAGCCCCTCTTACGCAGAGGCTCACCTCCTGATGGCGCAGGTGTACCT ATCCCAAGACAAAGTCCAGCTGTGTTCTCAGTCGCTTGAACTTTGTCTGAGCTACAATTTCAATGTGAGA GACTACCCACTGTACCACCTAATCAAAGCACAGTCACAGAAGAAAATGGGCGAGGTAGCAGAAGCAATTA AAACTCTGCACATGGCAATGAATCTACCAGGAATGAGGAGAAGTAGAGCTTCCTCGAAATCCAAGCACAG AAGTGAAGTGGATGGGAGCCATCGCTTATCCATCTTTCTTGAGTTGGTGGAGGTTCACCGCTTAAATGGA GAACAGCACGAGGCAGCAAAGGTTTTACAAGATGCCATCCATGAGTTTTCTGGAACTTGCGAAGAATTAC GTGTCACTATTGCTAATGCAGACCTGGCTCTGGCCCAAGGAGATACAGAACGTGCATTAAGCATGCTCCG AAATGTCACGACCGAACAGCCTTATTTCATAGAAGCCAAGGAAAAAATGGCAGACATTTACCTGAAGCAT AGGAAAGAGAAAATGTTGTACATCACTTGTTACAGAGAAATTGCTGAAAGGATGCCCAGCCCCCGGTCTT TCCTGCTCCTTGGTGATGCATACATGAACATTCAAGAGCCCGAGGAAGCCATAGTGGCCTATGAGCAAGC ATTAAATCAGAACCCAAAAGATGGAACACTGGCGAGAAAAATTGGGAAAGCACTTGTCAAAACTCACAAC TACTCAAAGGCAATCACCTATTATGAGGCTGCGCTGAAAAGTGGACAGCAGAATTGCCTGTGCTATGACC TGGCTGAGCTCCTCCTGAGACTAAAGTTGTATGAGAAAGCAGAGAAAGTTCTTCAGCATTCTCTAGCTCA TGATCCTGTAAATGAATTGTCGGCTCTCATGGTGGATGGGCGTTCCCAAGTCCTTCTAGCAAAAGTTTAC TCTAAAATGGAGAGACCTGGCGACGCGATTGCAGCATTGCAGCAGGCTCGAGAGCTGCAGGCTCGGATAC TGAAGCGAGTTCAGATGGAGCAGCCAGATGCGGTTCCCTCACAGAGACACTTCGCAGCTGAAATTTGTGC AGAGATCGCAAAACACTCCGCTGCTCAGCGAGACTACGAAAAGGCAATTACATTTTATAGAGAGGCCCTG GTTCATTGTGAAACGGATAGTAAGATAATGTTGGAACTGGCCCAGTTATACCTGGCCCAAGAAGACCTTG ATGCCTCCCTCCGGCACTGTGCACTGCTTCTCCAGAGGGACCAGGACAACGAACCGGCCACCATGCTGAT GGCCGACCTCATGTTCAGAAAACAGGACTATGAGCAAGCAGTGTTCCACTTGCAGCAGCTTTTAGAACGA AAACCAGATAATTTTATGACTTTGTCCCGTTTGATTGATCTCCTGAGAAGATGTGGGAAACTTGAGGAGG TTCCGAGATTTTTCTTGATGGCTGAGAAACACAACTCCAGAACAAAATTGGAGCCAGGATTTCAGTACTG TAAAGGATTACATTTTTGGTATACTGGAGAACCAAATGATGCCCTTCGACATTTTAATAAAGCTCGGAAA GATAGTGACTGGGGTCAAAATGCACTTTATAATATGATAGAAATCTGCTTGAATCCAGACAATGAAACTA TTGGAGGTGAAGTGTTTGAAAACCTGAATGGAGACCTGGGCACTTCCCCAGAGAAGCAGGAATCTGTGCA GTTAGCAGTGAGGACGGCAGAAAAGCTCCTTAAAGAACTAAAACCTCAGACTGTCCAGGGCCGCCTGCAA CTCCGCATCATGGAAAACTGCTGCTTAATGGCCACCAAGCAGAAGTCCAGTGTGGAGCAGGCGCTGAACA CCTTCACCGAAATAGCAGCCTCTGAGAAGGATCATATCCCTGCTCTCCTGGGAATGGCCACCGCTTATAT GATCTTGAAACAGACCCCGAAAGCCAGAAACCAGCTGAAGCGGATTGCAAAAATGACCTGGAATCCTATT GAAGCCGAGGAGCTGGAGAAGAGCTGGCTGCTGCTTGCGGATATTTATATCCAGTCAGCAAAATATGACA TGGCGGAAGAGCTGCTGAAACGGTGTCTGTGCCATAACCGGTCCTGCTGCAAAGCTTATGAATATATGGG GTACATTATGGAGAAGGAGCAAGCATACACAGATGCAGCCTTCAACTATGAGATGGCCTGGAAACATAGC AACCAGACAAATCCTGCTGTGGGATATAAATTGGCATTTAATTACTTAAAAGCAAAACGATATGTGGATG CAATTGACGTATGTCACCAGGTTCTTGAAGCACATCCAACTTACCCCAAAATCCGAAAGGATATACTGGA TAAGGCCCGTGCATCGTTAAGACCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191737
Insert Size	3948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191737.1, NP_001178666.1
RefSeq Size	3948 bp
RefSeq ORF	3948 bp
Locus ID	295654

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