Dhx57 (NM_001191907) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217610 representing NM_001191907 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTTCAGTGAGAAGAAAAGGCAAGCCAGGCAAAGGGGATGGAAAGGGGTCTTCTAGAGGAGGAA GAGGAGGCAGAGGACACGTGAATAAATCTCATGGGGGTGGAGGTAGTGGCGGCGGTGGCTGCGGCGGTGG CGGTGGTGGTGGCAGCGGCAGTAGAAAGGCCTCAAATAGAATTTGGGATGATGGCGATGACTTTTGTGTC TTCACTGAATCCAAGCGCCCGTCCAGGCCCTGCGACAGTAACAAAAGCAAAGGAGAAACACGGCCTAAGT GGAAGCCCAAAGCCAAAGTGCCCCTTCAGACGCTGCACATGACATCAGAGAATCAGGAGAAGGTGAAAGC CCTTCTGAGGGACCTGCAGGAACAGGGTGCGGACGCGGGATCCGAAAGAGGCTCCTCCGGGGAGGAGGAA GAGGACGCGCCCGAGGGCGCTGCCGAGCAGAGCTGGTCAGCTGGACCAGAGCCGACCTTTCTTCCCGACT GCCGTCCTTGGGAGTATATAGGCCCAGAAGAAGTGGAGCCTCCTCTTCCGGAATTTACAGTCTCGCCACT GGCAGTGCAAAAACTTTCCAGGTATGGCTTTCACACGGAGCACTGTCAGCTGGCCCTGAGGATCTGTGAG GGAGACTTAGGGGCAGCACTAGAGCACCTTCTGCACCAGTGCTTCTCGGAGACGTTTGGAGAGAGGATGG CACTGTCTGAGGCCGCTGTTTCTGTAAGCTTGGATGAGTGCGTGGAGCAGAGACAGGAAGAGACACTTGC TCTCAAGTCCATCTGTGGAGAAAAATTTATAGAAAGAATTCAGAACAGAGTCTGGACCATTGGATTGGAA CTGGAATATTTGACCAACAAATTCTGCAAGTCTAAGCAAAAGGAAAGTAACAAAAATGTACGGGACACTT CACCTGAAACCTGTAAATTTTACCTCAAAGGAAACTGTAAATTTGGATCAAAATGCAAATTCAAACATGA AGTGCCCCCACATCAGATCATTGGGAGAACGGAGAGAAACGTAAACGACCCTCATCTGGATGCTGAGAAC GACACCACTTTTCTGTATGAGCTTCAGATCCGCTTTTCTAAAGACCACAAATACCCCTACCAGGCTCCCC TCGTGGCGTTTTATTCCACCAATGAGAACCTACCTCTGGCTTGTCGTTTACATATTTCTGAGTTCCTTTA TGGCAAGGCCTTGGAGTTCGCAAAAACTTCAGAACCTGTTGTATATTCTTTGATAACCCTTTTAGAGGAA GAGTCTGAGATTGTCAAGTTACTAACACACACTCAACATAAGTACAGTGTTCCTCCCATCAACGTCCCTC CAGCACCATCCGAGACCAGAATAAGTAAACCTGCCTACCGCAAACCAGTGATCCCAAGTAACACTTTTCT TTCTAATCAGATGCTGGAAGGGGAAGGATTATCAGAGCTGGAGGAGGATGCAGATGAGGACGAAGGCCCT GCTCCAATTGTCGTGGAGAATGAAAGCTATGTGAACCTTAAGAAAAGGAGTTACAGAAGGTGTGACCGGC CTGCAAAGTCCTTATTTGCTGAGAACAGTAAGATCTGCAGGCAGTTCCAGATGAAACAGGCTTCCAGACA GTTCCACGCAATTCTGCAAGAAAGACAGTTACTACCTGCTTGGGAAGAAAGAGAAACCATTCTTAAATTG TTGAGCAAGCACCAAGTGGTTGTCATAAGTGGTATGACTGGATGTGGGAAAACCACACAGATTCCACAGT TTATTCTGGATAATTCTCTGAACGGGCCACCTGAGAGGGTGGCAAACATCATCTGTACCCAACCCCGACG AATCTCTGCGATCTCTGTTGCTGAGCGTGTTGCTAAGGAAAGAGCAGAAAGAGTGGGTCTGACGGTGGGC TATCAGATCCGGTTGGAAAGTGTCAAGTCCTCAGCCACCAGACTGTTATACTGCACCACAGGAGTGCTGC TGAGAAGGCTGGAAGGGGACGCAACTTTGCAGGGAGTCACCCACATCATTGTTGATGAAGTCCATGAGAG GACAGAAGAAAGTGACTTCTTACTGCTAGTGTTAAAGGACATTGTGATGCAGAGAGCAACTCTCCAGGTC ATTCTTATGAGTGCCACTCTGGACGCTGGGCTCTTCTCCAAGTATTTCAGCTACTGCCCTGTCATCACCA TACCAGGTCGTGCATTTCCTGTTGATCAGTTTTTTCTGGAGGATGCACTTGCCGTGACCAGGTATGTGTT ACAGGACGGGAGCCCGTATATGCGCTCCATGAAACAGATTGCGAAGGAAAGGCTTAAAGCCAGGCACAAC AGAACTGCACTGGAGGAGGTGGAAGAAGACCTGAGGCTGGCCCTTCACCTCCAGGACGAGGAGGAGTCTG TCAAAGACACAATACCTGATCAGCAGTTAGATTTCAAGCAGCTCTTGGTCCGTTATAAAGGGGTGAGCAA GTCGGTCATCAAGACAATGTCTGTCATGGATTTTGAAAAGGTCAACCTTGAATTAATAGAGGCCTTATTA GAGTGGATTGTAGACGGCAAGCACTCCTACCCTCCAGGTGCTATACTTGTGTTTTTACCAGGGCTAGCGG AAATCAAGATGCTTTATGAACAGCTACAGTCTAACTCTCTCTTCAACAACAGACGAAGTCATCGGTGTGT CATTCACCCACTGCACTCGTCGTTATCCAGTGAAGAGCAGCAGGCGGTGTTTGTGAAACCCCCACTAGGT GTGACTAAGATTATAATTTCCACCAACATTGCTGAGACTTCCATAACCATTGATGACGTCGTGTATGTCA TCGATTCTGGGAAGATGAAAGAAAAGAGATACGATGCCGGCAAAGGGATGGAAAGTCTAGAGGACACTTT TGTGTCTCAGGCCAATGCTCTGCAGAGAAAAGGCCGAGCTGGCCGAGTTGCCTCTGGAGTCTGCTTCCAC TTGTTCACGAGCCACCACTACAACCACCAGCTTCTGAAGCAGCAGCTCCCAGAGATCCAAAGGGTGCCCC TGGAGCAGCTGTGCCTCAGAATTAAAATTTTAGAAATGTTTAGTACTCACAATCTCCAATCCGTGTTCTC TCGGCTCATTGAACCTCCACATGTTGATTCTCTTCGTGCCTCAAAAGTACGGTTGCGAGACTTGGGAGCC TTGACTCCGGATGAAAAGCTCACCCCCCTGGGCTATCACTTGGCCTCTCTGCCTGTGGACGTTAGGATCG GCAAACTGATGTTGTTGGGATCAATCTTCCGCTGCCTGGACCCTGCTCTCACCATTGCTGCCAGCTTGGC TTTTAAGTCCCCTTTCGTGTCTCCCTGGGATAAGAAAGAAGAGGCCAATCAGAAAAAGCTGGAATTCGCA TTTGCAAACAGTGATTATCTGGCCCTTCTCTGTGCATATAAGGGTTGGCAGCTGAGTACAAAAGAAAGTG CGCGAGCAAGTTATAATTACTGCAGACAGAATTTCCTGTCTGGGAGAACTCTGCAGGAAATGGCCAGCCT CAAACGGCAGTTCACTGAGCTGCTTTCCGATATAGGGTTTGTCAAAGAGGGACTCAGAGCAAAAGAGATT GAAAAGAGAGCCCAAGGGGGAGATGGTGTCCTGGATGCCACAGGAGAGGAGGCTAACACAAATGCTGAGA ACCCCAAGCTGATATCAGCAGTGCTGTGTGCCGCTCTGTATCCAAATGTAGTACAGGTCAAAACCCCAGA AGGAAAGTTCCAGAAGACCAGTTCTGGAGTGGTCAGACTGCAGCCAAAGTCAGCTGAGCTGAAGTTCGTT ACCAAGAATGACGGCTATGTCCACATTCACCCTTCCTCAGTAAACTATCAGGTCAGACACTTCGACAGCC CCTACCTGTTGTACCATGAGAAAATCAAAACGAGCCGGGTGTTTATCCGAGACTGCAGCATGGTGTCTGT GTACCCCCTAGTCTTGTTTGGCGGAGGCCAAGTGAGTGTGCAGCTTCAAAGAGGGGCATTCGTCGTCTCC TTGGATGATGGGTGGATCCGGTTCGTGGCAGCTTCCCACCAGGTGGCCGAACTGGTGAAGGAACTCCGCT GTGAGCTTGACCAGCTCCTCCAGGATAAAATTAAGAACCCAAGCATGGACCTGTGTACCTGTCCTAGAGG GTCTCGAATTATCAGCATGATTGTGAAGCTGATCACCACACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191907
Insert Size	4176 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191907.2, NP_001178836.1
RefSeq Size	4674 bp
RefSeq ORF	4176 bp
Locus ID	366532

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