Pla2r1 (NM_001100837) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mrc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217632 representing NM_001100837 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAATGGTTAGCGATGCTCCAGCTGCTCTGGCTCCAGCAGCTGCTGCTGGGGGTTCACCAGGGCA GCGCCCAAGATTTGACCCACACCCAGGAACTGGCCCTCGAGTGGCGTGATAAAGGAATTTTCATTATCCA GAGCGAGAGCCTAAAGACTTGCATTCAGGCAGGTAAATCCGTACTGACCCTGGAGAACTGCACACAGCCA AACGAGTACATGCTGTGGAAATGGGTTTCCGACAACCACCTCTTTAATGTGGGAGCCAGCGGCTGCCTAG GCCTGAACATATCTGATCTGAAACGACCATTAAGTCTCTATGAATGTGATTCCACTCTCATTTCCTTGAG ATGGCTCTGTGACAGGAAGACGATCGTGGGCCCACTCCAGTACAAGGTCCAGGTGAACCACAGCACGGTG GTGGCAAAGAAAACACCCCATAAGTGGAGCGCCTACATGCCCGGTGGTGGGGACATTTGTGAGCATCTTC ACAGAGATATATATACCTTAAAAGGGAATGCCCATGGGATGCCTTGTGTGTTTCCTTTCCAGTTCAAAGG TCAGTGGCACCACGAGTGCATCCGAGAAGGGCATAAAGAGCACCTGCTGTGGTGTGCCACCACGAGTCGC TACGAAGAGGATGAGAAATGGGGATTCTGCCCAGATCCTACCTCTACAGAGATATTCTGTGATGCTATGT GGCAAAGGAACGACAATTCACACATTTGCTACCAATTCAACCTGCTCTCATCTCTCTCTTGGAATGAGGC ACATTCCTCATGCCAGATGCAAGGAGGTGCTTTGTTAAGTATTACGGATGAAAATGAAGAAGCTTTCATA AGGAAGGAGTTGAGCCGGGTAGCTAAAGAAGTGTGGACTGGTTTGAATCAGCTGGATGAGATGGCTGGCT GGCAGTGGTCTGATGGGACACCACTCAGCTACCTGAACTGGAGCCAAGAGGTCACTGCTGGGCCATTTGT GGAGTATCATTGTGGAACCCTGAAAGTTGCCTCAGCTACCTGGAGAAGCATGGACTGCGAGTCTACCTTG CCTTATATATGCAAACGGCATCTAAACCACACTGCCGAGGACATCCTGCAAAAGGACTCCTGGAAATACC ATACCACTCACTGTGACCCTGGCTGGACTCCCTTCAATCGTAAATGCTACAAACTTAAGAAAGAGAAAAG GACTTGGCAGGAGGCTCTGCACTCTTGCCAGTCTGATGACAGCATGTTGATGGATGTCACTTCCCTAGCT GAGGTGGAGTTGCTTGTAAACCTCCTTGGGGATGAAAATGCATCGGAAACATGGATTGGTTTGAGTAGCA ATAAAATTCCAGTTTCCTTCGCATGGTCCAGTGGCTCCTCTGTCATCTTCACTAACTGGTACCTTCTCGA GCCTCGAATTCTTCCAAACAGAAGACAACTGTGTGTCTCAGCAGATCAATCTGACGGACGCTGGAAAGTG AAAGGTTGTAAAGAAACACTCCCTTACATTTGTAAGAAAGCAGGACAAGTCCTTGAGGATGAGGACTCCG GATGTCAAGCGGGCTGGGAAAGACACGGCAAGTATTGTTACAAAATCGACACAGTCCTGAGAAGCTTTGA GCAGGCTTCCGGTGGTTATTACTGTCCTCCCGCCCTCCTAACCATCACCAGCCGATTTGAACAAGCTTTT ATTACCAGTTTGATCAGTAGTGTGGCAAGAAAGGACAGTTATTTTTGGATTGCTCTTCAAGACCAAAACA ATACAGGAGAATACACTTGGAAGACCATGGGACAGAGGGAGCCGGTGCAGTACACACACTGGAACGCACA TCAACCCAGCTACCATGGTGGCTGTGTTGTCCTGCGAGGAGGGAGTTCATTTGGTCGCTGGGAAGTGAAG AACTGTAGTGACTTTAAGGCAATGTCTCTTTGCAAGACACCCATCAAAGTCTGGGAGAAAACAGAGTTTG AAGGGAGGTGGCCCTTTCACCCCTGCTATATGGACTGGGAATCAGAGACTGGTCTAGCCAGTTGCTTCAA GGTCTTTCATAGCGAAAAGGTCCTGATGAAAAGATCATGGAGGGAAGCTGAAGCCTTCTGTGAGGAGTTT GGAGCTCACCTCGCAAGTTTTGCCCACATAGAGGAAGAAAACTTCGTGAATAAGCTTCTACACTCAAAAT TTAATTGGACACAAGAAAGGCAGTTCTGGATCGGATTTAATAAAAGGAACCCACTGAATGCCGGTTCTTG GGAGTGGTCTGACGGGTCTCCTGTTGTCTCTTCATTTTTAGACAATGCTTATTTTGAAGAAGATGCAAAA AACTGTGCTGTGTATAAGGCAAACAACACACTGTTGCCCTCGAACTGTGCTTCCAAACACGAGTGGATAT GCAAAATTCCAAGAGATGTGAAACCCAATTTTCCAGTCTGGTACCAGTACGATGCACCCTGGCTCTTCTA TCAGAATGCCGAGTACCTCTTCCACACCCATCCTGCCGAGTGGGCCACCTTTGAGTTTGTCTGCGGCTGG CTGCGAAGTGATTTCCTCACGATTTATTCTGCTCAGGAGCAAGAATTCATCCACAGCAAAATCAGAGCGC TATCAAAGTACGGGGCGAATTGGTGGATCGGACTTGAAGATAGAAGAGCTGGAGATCAAATTCACTGGAG TAATGGGTCACCCGTGATATTTCAGAACTGGGACAAAGGAAGGAAAGGAAGAATGGATAGCCAGAGAAAG CGATGCGTCTTCATGTCTGCTATAACAGGGCTCTGGGGCACTGAGAATTGCTCTGTTCCTATGCCCAGCA TCTGTAAGCGAGTGAAAATATGGGTCATCGAGAAGGAGAAGCCGCCAACACAGCATGGCACGTGCCCCAA AGGGTGGCTTTATTTTAACTATAAGTGCTTCCTGGTGACAATCCCCAAGGACCCACGGGAGCTGAAGACC TGGACAGGTGCTCAGGAATTCTGTGCTGCCAAGGGTGGGACGCTGGTCTCCATCGAAAGTGAACTGGAAC AAGCTTTCATTACTGTGAATCTTTTCGGCCAGACCACGAATGTATGGATAGGGTTACAAAGTGACAATCA TGAGAATTGGGTAAATGGGAAGCCTGTGGTGTATTCTAACTGGTCTCCATCTGATGTAATAAATATCCCC AATTATAACACCACTGAAGTTCAAAAGCGTGCTCCACTCTGTGCCCTGATGTCAAGTAATCCTAATTTCC ATTTTACTGGAAAATGGTATTTCGATGACTGTGGGAAAAAAGGCTATGGATTTGTGTGTGAAAAAATGCA AGATACCCTTGAAAACCATGTAAACGTGTCTGATACCTATGCAGTCCCCAGCACATTAGAATACGGAAAC AGGACTTATAAAATAATCCGTGCTAATATGACTTGGTACGCAGCAGGAAAGTCCTGCCGGATGCAGGGAG CAGAACTGGTCAGCATTCCAGACGTATTTCACCAGTCCTTCCTCACAGTTCTCCTCAGCAGGCTGGGGCA CGCCCACTGGATTGGGCTATCCACACCAGATAATGGTCTGAATTTTGACTGGTCAGACGGCACCAAATCC CCTTTCACCTATTGGAAAGATGAGGAGTCAGCCTCTCTTGGCGACTGCGTTTTTGCCGACACCGATGGTC ACTGGCATGGCACAGCCTGTGAGTCACATCTGCAAGGCGCCGTTTGTCGTGTACTCACTGAAACAAAGTC GTTTGAGCACCCAGTGCTGTGCTCAGAAACGTCAGTTCCCTGGATAAGATTTAAAGGTCATTGCTACAGT TTTTCTACAGTCCTGGACAGCAGAAGTTTTGAGGATGCTCGAGAATTCTGCAAAAGAGAAGGATCCAATC TTTTGGCAATCAAGGATGAGGCTGAAAATTTATTTCTCCTGGAAGAGCTTCTTGCCCTTGGTTCTTCTGT CCAGATGGTTTGGTTGAATGCTCAACTTGACAATGACAACAAAACCTTAAGGTGGTTTGACGGAGCAGCC ACAGAGCAGTCAAACTGGGGCGTACGGAAGCCAGACATGGACCACCTCAAGCCCCATCCGTGTGTTGTCC TGAGGATCCCTGAAGGAGTATGGCAGTCTACCCCATGCGAAGACAAAACGGGATTCATTTGTAAAGTGGA AGCAGGCATCCCAGCTGTAACAGCACATCCAGAGAAAGGACTAAGCCACAGCATCATGCCTGTTGCAGCA GCACTGGCGTTGACAATAGTCCTGGGCATTCTCGTCCTTTGCCTCTGGATTTACAAGCAGAATGGCGGTG CCATCTTGCCGAGACTCACGGGGTCGAGGGGCCCTTACTACCCTATGTCCAGCTTCAGCATGGCACACTT AGAAGAGAACATCCTCATTTCTGATCTTGAGAAGAATAACCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100837
Insert Size	4386 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100837.1, NP_001094307.1
RefSeq Size	5599 bp
RefSeq ORF	4386 bp
Locus ID	295631

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